Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia

Abstract. Ginting EL, Wantania LL, Moko EM, Tumbol RA, Siby MS, Wulur S. 2021. Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. Biodiversitas 22: 3326-3332. The aims of the research were to isolate and identify the amylase...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elvy Like Ginting, Letha Wantania, Emma Mauren Moko, Reiny Tumbol, Mayse Siby, Stenly Wullur
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	amylase, bacillus, identification, likupang, thermophilic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5e7f6d683f0d4899ac82b6efa5e80fb6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5e7f6d683f0d4899ac82b6efa5e80fb6
record_format	dspace
spelling	oai:doaj.org-article:5e7f6d683f0d4899ac82b6efa5e80fb62021-11-22T12:14:18ZIsolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia1412-033X2085-472210.13057/biodiv/d220638https://doaj.org/article/5e7f6d683f0d4899ac82b6efa5e80fb62021-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8296https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ginting EL, Wantania LL, Moko EM, Tumbol RA, Siby MS, Wulur S. 2021. Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. Biodiversitas 22: 3326-3332. The aims of the research were to isolate and identify the amylase-producing thermophilic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. The bacteria were characterized based on the colony and cell morphology and subsequently screened for their amylase activities. The bacterial isolates were identified based on 16S rRNA gene sequences. There were 12 thermophilic bacteria isolates from Likupang Marine Hydrothermal that were able to produce amylase. Two selected isolates (L3 and L9) had an amylolytic index value in the range of 3.04-3.52 at 55oC. The colonies of L3 and L9 are circular, and they are Gram positive, rod-shaped, and motile bacteria. Based on the 16S rRNA gene sequences and phylogenetic tree analysis showed that L3 was closely related to Bacillus caldotenax with 93% similarity while L9 had 99% similarity to B. caldotenax. Hence, we identified L3 isolate as Bacillus sp. strain L3 and L9 isolate as B. caldotenax strain L9. Both of these bacteria were grouped as Geobacillus thermoleovorans.Elvy Like GintingLetha WantaniaEmma Mauren MokoReiny TumbolMayse SibyStenly WullurMBI & UNS Soloarticleamylase, bacillus, identification, likupang, thermophilicBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amylase, bacillus, identification, likupang, thermophilic Biology (General) QH301-705.5
spellingShingle	amylase, bacillus, identification, likupang, thermophilic Biology (General) QH301-705.5 Elvy Like Ginting Letha Wantania Emma Mauren Moko Reiny Tumbol Mayse Siby Stenly Wullur Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia
description	Abstract. Ginting EL, Wantania LL, Moko EM, Tumbol RA, Siby MS, Wulur S. 2021. Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. Biodiversitas 22: 3326-3332. The aims of the research were to isolate and identify the amylase-producing thermophilic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. The bacteria were characterized based on the colony and cell morphology and subsequently screened for their amylase activities. The bacterial isolates were identified based on 16S rRNA gene sequences. There were 12 thermophilic bacteria isolates from Likupang Marine Hydrothermal that were able to produce amylase. Two selected isolates (L3 and L9) had an amylolytic index value in the range of 3.04-3.52 at 55oC. The colonies of L3 and L9 are circular, and they are Gram positive, rod-shaped, and motile bacteria. Based on the 16S rRNA gene sequences and phylogenetic tree analysis showed that L3 was closely related to Bacillus caldotenax with 93% similarity while L9 had 99% similarity to B. caldotenax. Hence, we identified L3 isolate as Bacillus sp. strain L3 and L9 isolate as B. caldotenax strain L9. Both of these bacteria were grouped as Geobacillus thermoleovorans.
format	article
author	Elvy Like Ginting Letha Wantania Emma Mauren Moko Reiny Tumbol Mayse Siby Stenly Wullur
author_facet	Elvy Like Ginting Letha Wantania Emma Mauren Moko Reiny Tumbol Mayse Siby Stenly Wullur
author_sort	Elvy Like Ginting
title	Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia
title_short	Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia
title_full	Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia
title_fullStr	Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia
title_full_unstemmed	Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia
title_sort	isolation and identification of thermophilic amylolytic bacteria from likupang marine hydrothermal, north sulawesi, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/5e7f6d683f0d4899ac82b6efa5e80fb6
work_keys_str_mv	AT elvylikeginting isolationandidentificationofthermophilicamylolyticbacteriafromlikupangmarinehydrothermalnorthsulawesiindonesia AT lethawantania isolationandidentificationofthermophilicamylolyticbacteriafromlikupangmarinehydrothermalnorthsulawesiindonesia AT emmamaurenmoko isolationandidentificationofthermophilicamylolyticbacteriafromlikupangmarinehydrothermalnorthsulawesiindonesia AT reinytumbol isolationandidentificationofthermophilicamylolyticbacteriafromlikupangmarinehydrothermalnorthsulawesiindonesia AT maysesiby isolationandidentificationofthermophilicamylolyticbacteriafromlikupangmarinehydrothermalnorthsulawesiindonesia AT stenlywullur isolationandidentificationofthermophilicamylolyticbacteriafromlikupangmarinehydrothermalnorthsulawesiindonesia
_version_	1718417677987348480

Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia

Ejemplares similares