Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.

<h4>Background</h4>Ozone concentrations are predicted to increase over the next 50 years due to global warming and the increased release of precursor chemicals. It is therefore urgent that good, reliable biomarkers are available to quantify the toxicity of this pollutant gas at the popul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jenny A Bosson, Anders Blomberg, Nikolai Stenfors, Ragnberth Helleday, Frank J Kelly, Annelie F Behndig, Ian S Mudway
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e8594836e68471ca7732787ec1b37cc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5e8594836e68471ca7732787ec1b37cc
record_format	dspace
spelling	oai:doaj.org-article:5e8594836e68471ca7732787ec1b37cc2021-11-18T08:39:42ZPeripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.1932-620310.1371/journal.pone.0081816https://doaj.org/article/5e8594836e68471ca7732787ec1b37cc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391708/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Ozone concentrations are predicted to increase over the next 50 years due to global warming and the increased release of precursor chemicals. It is therefore urgent that good, reliable biomarkers are available to quantify the toxicity of this pollutant gas at the population level. Such a biomarker would need to be easily performed, reproducible, economically viable, and reflective of ongoing pathological processes occurring within the lung.<h4>Methodology</h4>We examined whether blood neutrophilia occurred following a controlled ozone challenge and addressed whether this could serve as a biomarker for ozone-induced airway inflammation. Three separate groups of healthy subjects were exposed to ozone (0.2 ppm, 2h) and filtered air (FA) on two separate occasions. Peripheral blood samples were collected and bronchoscopy with biopsy sampling and lavages was performed at 1.5h post exposures in group 1 (n=13), at 6h in group 2 (n=15) and at 18h in group 3 (n=15). Total and differential cell counts were assessed in blood, bronchial tissue and airway lavages.<h4>Results</h4>In peripheral blood, we observed fewer neutrophils 1.5h after ozone compared with the parallel air exposure (-1.1±1.0x10(9) cells/L, p<0.01), at 6h neutrophil numbers were increased compared to FA (+1.2±1.3x10(9) cells/L, p<0.01), and at 18h this response had fully attenuated. Ozone induced a peak in neutrophil numbers at 6h post exposure in all compartments examined, with a positive correlation between the response in blood and bronchial biopsies.<h4>Conclusions</h4>These data demonstrate a systemic neutrophilia in healthy subjects following an acute ozone exposure, which mirrors the inflammatory response in the lung mucosa and lumen. This relationship suggests that blood neutrophilia could be used as a relatively simple functional biomarker for the effect of ozone on the lung.Jenny A BossonAnders BlombergNikolai StenforsRagnberth HelledayFrank J KellyAnnelie F BehndigIan S MudwayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81816 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jenny A Bosson Anders Blomberg Nikolai Stenfors Ragnberth Helleday Frank J Kelly Annelie F Behndig Ian S Mudway Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
description	<h4>Background</h4>Ozone concentrations are predicted to increase over the next 50 years due to global warming and the increased release of precursor chemicals. It is therefore urgent that good, reliable biomarkers are available to quantify the toxicity of this pollutant gas at the population level. Such a biomarker would need to be easily performed, reproducible, economically viable, and reflective of ongoing pathological processes occurring within the lung.<h4>Methodology</h4>We examined whether blood neutrophilia occurred following a controlled ozone challenge and addressed whether this could serve as a biomarker for ozone-induced airway inflammation. Three separate groups of healthy subjects were exposed to ozone (0.2 ppm, 2h) and filtered air (FA) on two separate occasions. Peripheral blood samples were collected and bronchoscopy with biopsy sampling and lavages was performed at 1.5h post exposures in group 1 (n=13), at 6h in group 2 (n=15) and at 18h in group 3 (n=15). Total and differential cell counts were assessed in blood, bronchial tissue and airway lavages.<h4>Results</h4>In peripheral blood, we observed fewer neutrophils 1.5h after ozone compared with the parallel air exposure (-1.1±1.0x10(9) cells/L, p<0.01), at 6h neutrophil numbers were increased compared to FA (+1.2±1.3x10(9) cells/L, p<0.01), and at 18h this response had fully attenuated. Ozone induced a peak in neutrophil numbers at 6h post exposure in all compartments examined, with a positive correlation between the response in blood and bronchial biopsies.<h4>Conclusions</h4>These data demonstrate a systemic neutrophilia in healthy subjects following an acute ozone exposure, which mirrors the inflammatory response in the lung mucosa and lumen. This relationship suggests that blood neutrophilia could be used as a relatively simple functional biomarker for the effect of ozone on the lung.
format	article
author	Jenny A Bosson Anders Blomberg Nikolai Stenfors Ragnberth Helleday Frank J Kelly Annelie F Behndig Ian S Mudway
author_facet	Jenny A Bosson Anders Blomberg Nikolai Stenfors Ragnberth Helleday Frank J Kelly Annelie F Behndig Ian S Mudway
author_sort	Jenny A Bosson
title	Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
title_short	Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
title_full	Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
title_fullStr	Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
title_full_unstemmed	Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
title_sort	peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5e8594836e68471ca7732787ec1b37cc
work_keys_str_mv	AT jennyabosson peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation AT andersblomberg peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation AT nikolaistenfors peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation AT ragnberthhelleday peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation AT frankjkelly peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation AT anneliefbehndig peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation AT iansmudway peripheralbloodneutrophiliaasabiomarkerofozoneinducedpulmonaryinflammation
_version_	1718421502693474304

Peripheral blood neutrophilia as a biomarker of ozone-induced pulmonary inflammation.

Ejemplares similares