Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces...

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Autores principales:	Pedro Beltrao, Jonathan C Trinidad, Dorothea Fiedler, Assen Roguev, Wendell A Lim, Kevan M Shokat, Alma L Burlingame, Nevan J Krogan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d
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spelling	oai:doaj.org-article:5e8bdd4a44c841e89f6e26ba4767285d2021-11-25T05:34:08ZEvolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.1544-91731545-788510.1371/journal.pbio.1000134https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d2009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.Pedro BeltraoJonathan C TrinidadDorothea FiedlerAssen RoguevWendell A LimKevan M ShokatAlma L BurlingameNevan J KroganPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 6, p e1000134 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Pedro Beltrao Jonathan C Trinidad Dorothea Fiedler Assen Roguev Wendell A Lim Kevan M Shokat Alma L Burlingame Nevan J Krogan Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
description	The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.
format	article
author	Pedro Beltrao Jonathan C Trinidad Dorothea Fiedler Assen Roguev Wendell A Lim Kevan M Shokat Alma L Burlingame Nevan J Krogan
author_facet	Pedro Beltrao Jonathan C Trinidad Dorothea Fiedler Assen Roguev Wendell A Lim Kevan M Shokat Alma L Burlingame Nevan J Krogan
author_sort	Pedro Beltrao
title	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
title_short	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
title_full	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
title_fullStr	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
title_full_unstemmed	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
title_sort	evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d
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Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

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