Deep tissue analysis of distal aqueous drainage structures and contractile features

Abstract Outflow resistance in the aqueous drainage tract distal to trabecular meshwork is potentially an important determinant of intraocular pressure and success of trabecular bypass glaucoma surgeries. It is unclear how distal resistance is modulated. We sought to establish: (a) multimodal 2-phot...

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Autores principales:	Jose M. Gonzalez, Minhee K. Ko, Young-Kwon Hong, Robert Weigert, James C. H. Tan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5e9d8604e15246c3a3f8ff47cabada08
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spelling	oai:doaj.org-article:5e9d8604e15246c3a3f8ff47cabada082021-12-02T15:05:05ZDeep tissue analysis of distal aqueous drainage structures and contractile features10.1038/s41598-017-16897-y2045-2322https://doaj.org/article/5e9d8604e15246c3a3f8ff47cabada082017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-16897-yhttps://doaj.org/toc/2045-2322Abstract Outflow resistance in the aqueous drainage tract distal to trabecular meshwork is potentially an important determinant of intraocular pressure and success of trabecular bypass glaucoma surgeries. It is unclear how distal resistance is modulated. We sought to establish: (a) multimodal 2-photon deep tissue imaging and 3-dimensional analysis of the distal aqueous drainage tract (DT) in transgenic mice in vivo and ex vivo; (b) criteria for distinguishing the DT from blood and lymphatic vessels; and (c) presence of a DT wall organization capable of contractility. DT lumen appeared as scleral collagen second harmonic generation signal voids that could be traced back to Schlemm’s canal. DT endothelium was Prox1-positive, CD31-positive and LYVE-1-negative, bearing a different molecular signature from blood and true lymphatic vessels. DT walls showed prominent filamentous actin (F-actin) labeling reflecting cells in a contracted state. F-actin co-localized with mesenchymal smooth muscle epitopes of alpha-smooth muscle actin, caldesmon and calponin, which localized adjacent and external to the endothelium. Our findings support a DT wall organization resembling that of blood vessels. This reflects a capacity to contract and support dynamic alteration of DT caliber and resistance analogous to the role of blood vessel tone in regulating blood flow.Jose M. GonzalezMinhee K. KoYoung-Kwon HongRobert WeigertJames C. H. TanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-20 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jose M. Gonzalez Minhee K. Ko Young-Kwon Hong Robert Weigert James C. H. Tan Deep tissue analysis of distal aqueous drainage structures and contractile features
description	Abstract Outflow resistance in the aqueous drainage tract distal to trabecular meshwork is potentially an important determinant of intraocular pressure and success of trabecular bypass glaucoma surgeries. It is unclear how distal resistance is modulated. We sought to establish: (a) multimodal 2-photon deep tissue imaging and 3-dimensional analysis of the distal aqueous drainage tract (DT) in transgenic mice in vivo and ex vivo; (b) criteria for distinguishing the DT from blood and lymphatic vessels; and (c) presence of a DT wall organization capable of contractility. DT lumen appeared as scleral collagen second harmonic generation signal voids that could be traced back to Schlemm’s canal. DT endothelium was Prox1-positive, CD31-positive and LYVE-1-negative, bearing a different molecular signature from blood and true lymphatic vessels. DT walls showed prominent filamentous actin (F-actin) labeling reflecting cells in a contracted state. F-actin co-localized with mesenchymal smooth muscle epitopes of alpha-smooth muscle actin, caldesmon and calponin, which localized adjacent and external to the endothelium. Our findings support a DT wall organization resembling that of blood vessels. This reflects a capacity to contract and support dynamic alteration of DT caliber and resistance analogous to the role of blood vessel tone in regulating blood flow.
format	article
author	Jose M. Gonzalez Minhee K. Ko Young-Kwon Hong Robert Weigert James C. H. Tan
author_facet	Jose M. Gonzalez Minhee K. Ko Young-Kwon Hong Robert Weigert James C. H. Tan
author_sort	Jose M. Gonzalez
title	Deep tissue analysis of distal aqueous drainage structures and contractile features
title_short	Deep tissue analysis of distal aqueous drainage structures and contractile features
title_full	Deep tissue analysis of distal aqueous drainage structures and contractile features
title_fullStr	Deep tissue analysis of distal aqueous drainage structures and contractile features
title_full_unstemmed	Deep tissue analysis of distal aqueous drainage structures and contractile features
title_sort	deep tissue analysis of distal aqueous drainage structures and contractile features
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5e9d8604e15246c3a3f8ff47cabada08
work_keys_str_mv	AT josemgonzalez deeptissueanalysisofdistalaqueousdrainagestructuresandcontractilefeatures AT minheekko deeptissueanalysisofdistalaqueousdrainagestructuresandcontractilefeatures AT youngkwonhong deeptissueanalysisofdistalaqueousdrainagestructuresandcontractilefeatures AT robertweigert deeptissueanalysisofdistalaqueousdrainagestructuresandcontractilefeatures AT jameschtan deeptissueanalysisofdistalaqueousdrainagestructuresandcontractilefeatures
_version_	1718388958417649664

Deep tissue analysis of distal aqueous drainage structures and contractile features

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