Multiscale cytometry and regulation of 3D cell cultures on a chip

3D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of indi...

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Bibliographic Details
Main Authors: Sébastien Sart, Raphaël F.-X. Tomasi, Gabriel Amselem, Charles N. Baroud
Format: article
Language:EN
Published: Nature Portfolio 2017
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Online Access:https://doaj.org/article/5ea8ecb0c84a439a84b3ea4a52ffe332
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Summary:3D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of individual cells within the spheroid.