Multiscale cytometry and regulation of 3D cell cultures on a chip
3D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of indi...
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Nature Portfolio
2017
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oai:doaj.org-article:5ea8ecb0c84a439a84b3ea4a52ffe3322021-12-02T17:06:03ZMultiscale cytometry and regulation of 3D cell cultures on a chip10.1038/s41467-017-00475-x2041-1723https://doaj.org/article/5ea8ecb0c84a439a84b3ea4a52ffe3322017-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00475-xhttps://doaj.org/toc/2041-17233D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of individual cells within the spheroid.Sébastien SartRaphaël F.-X. TomasiGabriel AmselemCharles N. BaroudNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-13 (2017) |
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Science Q Sébastien Sart Raphaël F.-X. Tomasi Gabriel Amselem Charles N. Baroud Multiscale cytometry and regulation of 3D cell cultures on a chip |
description |
3D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of individual cells within the spheroid. |
format |
article |
author |
Sébastien Sart Raphaël F.-X. Tomasi Gabriel Amselem Charles N. Baroud |
author_facet |
Sébastien Sart Raphaël F.-X. Tomasi Gabriel Amselem Charles N. Baroud |
author_sort |
Sébastien Sart |
title |
Multiscale cytometry and regulation of 3D cell cultures on a chip |
title_short |
Multiscale cytometry and regulation of 3D cell cultures on a chip |
title_full |
Multiscale cytometry and regulation of 3D cell cultures on a chip |
title_fullStr |
Multiscale cytometry and regulation of 3D cell cultures on a chip |
title_full_unstemmed |
Multiscale cytometry and regulation of 3D cell cultures on a chip |
title_sort |
multiscale cytometry and regulation of 3d cell cultures on a chip |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/5ea8ecb0c84a439a84b3ea4a52ffe332 |
work_keys_str_mv |
AT sebastiensart multiscalecytometryandregulationof3dcellculturesonachip AT raphaelfxtomasi multiscalecytometryandregulationof3dcellculturesonachip AT gabrielamselem multiscalecytometryandregulationof3dcellculturesonachip AT charlesnbaroud multiscalecytometryandregulationof3dcellculturesonachip |
_version_ |
1718381731347693568 |