Multiscale cytometry and regulation of 3D cell cultures on a chip

3D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of indi...

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Autores principales: Sébastien Sart, Raphaël F.-X. Tomasi, Gabriel Amselem, Charles N. Baroud
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/5ea8ecb0c84a439a84b3ea4a52ffe332
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spelling oai:doaj.org-article:5ea8ecb0c84a439a84b3ea4a52ffe3322021-12-02T17:06:03ZMultiscale cytometry and regulation of 3D cell cultures on a chip10.1038/s41467-017-00475-x2041-1723https://doaj.org/article/5ea8ecb0c84a439a84b3ea4a52ffe3322017-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00475-xhttps://doaj.org/toc/2041-17233D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of individual cells within the spheroid.Sébastien SartRaphaël F.-X. TomasiGabriel AmselemCharles N. BaroudNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sébastien Sart
Raphaël F.-X. Tomasi
Gabriel Amselem
Charles N. Baroud
Multiscale cytometry and regulation of 3D cell cultures on a chip
description 3D cell culture is more relevant than the two-dimensional format, but methods for parallel analysis and temporal regulation of the microenvironment are limited. Here the authors develop a droplet microfluidics system to perform long-term culture of 3D spheroids, enabling multiscale cytometry of individual cells within the spheroid.
format article
author Sébastien Sart
Raphaël F.-X. Tomasi
Gabriel Amselem
Charles N. Baroud
author_facet Sébastien Sart
Raphaël F.-X. Tomasi
Gabriel Amselem
Charles N. Baroud
author_sort Sébastien Sart
title Multiscale cytometry and regulation of 3D cell cultures on a chip
title_short Multiscale cytometry and regulation of 3D cell cultures on a chip
title_full Multiscale cytometry and regulation of 3D cell cultures on a chip
title_fullStr Multiscale cytometry and regulation of 3D cell cultures on a chip
title_full_unstemmed Multiscale cytometry and regulation of 3D cell cultures on a chip
title_sort multiscale cytometry and regulation of 3d cell cultures on a chip
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/5ea8ecb0c84a439a84b3ea4a52ffe332
work_keys_str_mv AT sebastiensart multiscalecytometryandregulationof3dcellculturesonachip
AT raphaelfxtomasi multiscalecytometryandregulationof3dcellculturesonachip
AT gabrielamselem multiscalecytometryandregulationof3dcellculturesonachip
AT charlesnbaroud multiscalecytometryandregulationof3dcellculturesonachip
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