Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats

Abstract Methylmercury, biomagnifying through food chains, is highly toxic for aquatic life. Its production and degradation are largely driven by microbial transformations; however, diversity and metabolic activity of mercury transformers, resulting in methylmercury concentrations in environments, r...

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Autores principales:	Adrien Vigneron, Perrine Cruaud, Johanne Aubé, Rémy Guyoneaud, Marisol Goñi-Urriza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/5eaa0efa111f45a3b08494bf5e47553b
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spelling	oai:doaj.org-article:5eaa0efa111f45a3b08494bf5e47553b2021-11-21T12:26:19ZTranscriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats10.1038/s41522-021-00255-y2055-5008https://doaj.org/article/5eaa0efa111f45a3b08494bf5e47553b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00255-yhttps://doaj.org/toc/2055-5008Abstract Methylmercury, biomagnifying through food chains, is highly toxic for aquatic life. Its production and degradation are largely driven by microbial transformations; however, diversity and metabolic activity of mercury transformers, resulting in methylmercury concentrations in environments, remain poorly understood. Microbial mats are thick biofilms where oxic and anoxic metabolisms cooccur, providing opportunities to investigate the complexity of the microbial mercury transformations over contrasted redox conditions. Here, we conducted a genome-resolved metagenomic and metatranscriptomic analysis to identify putative activity of mercury reducers, methylators and demethylators in microbial mats strongly contaminated by mercury. Our transcriptomic results revealed the major role of rare microorganisms in mercury cycling. Mercury methylators, mainly related to Desulfobacterota, expressed a large panel of metabolic activities in sulfur, iron, nitrogen, and halogen compound transformations, extending known activities of mercury methylators under suboxic to anoxic conditions. Methylmercury detoxification processes were dissociated in the microbial mats with methylmercury cleavage being carried out by sulfide-oxidizing Thiotrichaceae and Rhodobacteraceae populations, whereas mercury reducers included members of the Verrucomicrobia, Bacteroidetes, Gammaproteobacteria, and different populations of Rhodobacteraceae. However most of the mercury reduction was potentially carried out anaerobically by sulfur- and iron-reducing Desulfuromonadaceae, revising our understanding of mercury transformers ecophysiology.Adrien VigneronPerrine CruaudJohanne AubéRémy GuyoneaudMarisol Goñi-UrrizaNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Adrien Vigneron Perrine Cruaud Johanne Aubé Rémy Guyoneaud Marisol Goñi-Urriza Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
description	Abstract Methylmercury, biomagnifying through food chains, is highly toxic for aquatic life. Its production and degradation are largely driven by microbial transformations; however, diversity and metabolic activity of mercury transformers, resulting in methylmercury concentrations in environments, remain poorly understood. Microbial mats are thick biofilms where oxic and anoxic metabolisms cooccur, providing opportunities to investigate the complexity of the microbial mercury transformations over contrasted redox conditions. Here, we conducted a genome-resolved metagenomic and metatranscriptomic analysis to identify putative activity of mercury reducers, methylators and demethylators in microbial mats strongly contaminated by mercury. Our transcriptomic results revealed the major role of rare microorganisms in mercury cycling. Mercury methylators, mainly related to Desulfobacterota, expressed a large panel of metabolic activities in sulfur, iron, nitrogen, and halogen compound transformations, extending known activities of mercury methylators under suboxic to anoxic conditions. Methylmercury detoxification processes were dissociated in the microbial mats with methylmercury cleavage being carried out by sulfide-oxidizing Thiotrichaceae and Rhodobacteraceae populations, whereas mercury reducers included members of the Verrucomicrobia, Bacteroidetes, Gammaproteobacteria, and different populations of Rhodobacteraceae. However most of the mercury reduction was potentially carried out anaerobically by sulfur- and iron-reducing Desulfuromonadaceae, revising our understanding of mercury transformers ecophysiology.
format	article
author	Adrien Vigneron Perrine Cruaud Johanne Aubé Rémy Guyoneaud Marisol Goñi-Urriza
author_facet	Adrien Vigneron Perrine Cruaud Johanne Aubé Rémy Guyoneaud Marisol Goñi-Urriza
author_sort	Adrien Vigneron
title	Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
title_short	Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
title_full	Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
title_fullStr	Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
title_full_unstemmed	Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
title_sort	transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5eaa0efa111f45a3b08494bf5e47553b
work_keys_str_mv	AT adrienvigneron transcriptomicevidenceforversatilemetabolicactivitiesofmercurycyclingmicroorganismsinbrackishmicrobialmats AT perrinecruaud transcriptomicevidenceforversatilemetabolicactivitiesofmercurycyclingmicroorganismsinbrackishmicrobialmats AT johanneaube transcriptomicevidenceforversatilemetabolicactivitiesofmercurycyclingmicroorganismsinbrackishmicrobialmats AT remyguyoneaud transcriptomicevidenceforversatilemetabolicactivitiesofmercurycyclingmicroorganismsinbrackishmicrobialmats AT marisolgoniurriza transcriptomicevidenceforversatilemetabolicactivitiesofmercurycyclingmicroorganismsinbrackishmicrobialmats
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Transcriptomic evidence for versatile metabolic activities of mercury cycling microorganisms in brackish microbial mats

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