Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus

Abstract GETV, an arbo-borne zoonotic virus of the genus Alphavirus, which causes diarrhea and reproduction disorders in swine, lead to serious economic losses to the swine industry in China. At present, the existing methods for GETV detection are time-consuming and low sensitivity, so, a rapid, acc...

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Autores principales:	Mincai Nie, Huidan Deng, Yuancheng Zhou, Xiangang Sun, Yao Huang, Ling Zhu, Zhiwen Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5eaa71723b914f4b921967b39dc2736f
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spelling	oai:doaj.org-article:5eaa71723b914f4b921967b39dc2736f2021-12-02T18:37:09ZDevelopment of a reverse transcription recombinase-aided amplification assay for detection of Getah virus10.1038/s41598-021-99734-72045-2322https://doaj.org/article/5eaa71723b914f4b921967b39dc2736f2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99734-7https://doaj.org/toc/2045-2322Abstract GETV, an arbo-borne zoonotic virus of the genus Alphavirus, which causes diarrhea and reproduction disorders in swine, lead to serious economic losses to the swine industry in China. At present, the existing methods for GETV detection are time-consuming and low sensitivity, so, a rapid, accurate and sensitive GETV detection method is urgently needed. In this study, a fluorescent reverse transcription recombinase-assisted amplification method (RT-RAA) was successfully established for the rapid detection of GETV. The sensitivity of this method to GETV was 8 copies/reaction and 20 TCID50/reaction. No cross-reaction with other viruses. A total of 118 samples were prepared for GETV detection using fluorescent RT-RAA and SYBR Green I RT-qPCR, the coincidence rate of the two methods was 100%. The results suggest that the RT-RAA method is rapid, sensitive and specific for GETV detection and can be applied in the clinical.Mincai NieHuidan DengYuancheng ZhouXiangang SunYao HuangLing ZhuZhiwen XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mincai Nie Huidan Deng Yuancheng Zhou Xiangang Sun Yao Huang Ling Zhu Zhiwen Xu Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus
description	Abstract GETV, an arbo-borne zoonotic virus of the genus Alphavirus, which causes diarrhea and reproduction disorders in swine, lead to serious economic losses to the swine industry in China. At present, the existing methods for GETV detection are time-consuming and low sensitivity, so, a rapid, accurate and sensitive GETV detection method is urgently needed. In this study, a fluorescent reverse transcription recombinase-assisted amplification method (RT-RAA) was successfully established for the rapid detection of GETV. The sensitivity of this method to GETV was 8 copies/reaction and 20 TCID50/reaction. No cross-reaction with other viruses. A total of 118 samples were prepared for GETV detection using fluorescent RT-RAA and SYBR Green I RT-qPCR, the coincidence rate of the two methods was 100%. The results suggest that the RT-RAA method is rapid, sensitive and specific for GETV detection and can be applied in the clinical.
format	article
author	Mincai Nie Huidan Deng Yuancheng Zhou Xiangang Sun Yao Huang Ling Zhu Zhiwen Xu
author_facet	Mincai Nie Huidan Deng Yuancheng Zhou Xiangang Sun Yao Huang Ling Zhu Zhiwen Xu
author_sort	Mincai Nie
title	Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus
title_short	Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus
title_full	Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus
title_fullStr	Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus
title_full_unstemmed	Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus
title_sort	development of a reverse transcription recombinase-aided amplification assay for detection of getah virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5eaa71723b914f4b921967b39dc2736f
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Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus

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