The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology
Sheng-Ji Hua-Yu (SJHY) formula has been proved to reduce the severity of diabetic wound healing without significant adverse events in our previous clinical trials. However, based on multi-target characteristics, the regulatory network among herbs, ingredients, and hub genes remains to be elucidated....
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Frontiers Media S.A.
2021
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oai:doaj.org-article:5eb3df2495f249ac8f0def9c38b954c72021-11-16T07:39:18ZThe Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology1663-981210.3389/fphar.2021.726158https://doaj.org/article/5eb3df2495f249ac8f0def9c38b954c72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.726158/fullhttps://doaj.org/toc/1663-9812Sheng-Ji Hua-Yu (SJHY) formula has been proved to reduce the severity of diabetic wound healing without significant adverse events in our previous clinical trials. However, based on multi-target characteristics, the regulatory network among herbs, ingredients, and hub genes remains to be elucidated. The current study aims to identify the biomarkers of the SJHY formula for the treatment of diabetic wound healing. First, a network of components and targets for the SJHY formula was constructed using network pharmacology. Second, the ClusterONE algorithm was used to build a modular network and identify hub genes along with kernel pathways. Third, we verified the kernel targets by molecular docking to select hub genes. In addition, the biomarkers of the SJHY formula were validated by animal experiments in a diabetic wound healing mice model. The results revealed that the SJHY formula downregulated the mRNA expression of Cxcr4, Oprd1, and Htr2a, while upregulated Adrb2, Drd, Drd4, and Hrh1. Besides, the SJHY formula upregulated the kernel pathways, neuroactive ligand–receptor interaction, and cAMP signaling pathway in the skin tissue homogenate of the diabetic wound healing mice model. In summary, this study identified the potential targets and kernel pathways, providing additional evidence for the clinical application of the SJHY formula for the treatment of diabetic wound healing.Jing-Si JiangJing-Si JiangYing ZhangYing ZhangYing LuoYing LuoYi RuYue LuoXiao-Ya FeiJian-Kun SongXiao-Jie DingXiao-Jie DingZhan ZhangDan YangShuang-Yi YinHui-Ping ZhangTai-Yi LiuBin LiBin LiBin LiLe KuaiLe KuaiFrontiers Media S.A.articlediabetic wound healingSJHY formulamodular pharmacologymolecular dockingexperimental verificationTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021) |
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diabetic wound healing SJHY formula modular pharmacology molecular docking experimental verification Therapeutics. Pharmacology RM1-950 |
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diabetic wound healing SJHY formula modular pharmacology molecular docking experimental verification Therapeutics. Pharmacology RM1-950 Jing-Si Jiang Jing-Si Jiang Ying Zhang Ying Zhang Ying Luo Ying Luo Yi Ru Yue Luo Xiao-Ya Fei Jian-Kun Song Xiao-Jie Ding Xiao-Jie Ding Zhan Zhang Dan Yang Shuang-Yi Yin Hui-Ping Zhang Tai-Yi Liu Bin Li Bin Li Bin Li Le Kuai Le Kuai The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology |
| description |
Sheng-Ji Hua-Yu (SJHY) formula has been proved to reduce the severity of diabetic wound healing without significant adverse events in our previous clinical trials. However, based on multi-target characteristics, the regulatory network among herbs, ingredients, and hub genes remains to be elucidated. The current study aims to identify the biomarkers of the SJHY formula for the treatment of diabetic wound healing. First, a network of components and targets for the SJHY formula was constructed using network pharmacology. Second, the ClusterONE algorithm was used to build a modular network and identify hub genes along with kernel pathways. Third, we verified the kernel targets by molecular docking to select hub genes. In addition, the biomarkers of the SJHY formula were validated by animal experiments in a diabetic wound healing mice model. The results revealed that the SJHY formula downregulated the mRNA expression of Cxcr4, Oprd1, and Htr2a, while upregulated Adrb2, Drd, Drd4, and Hrh1. Besides, the SJHY formula upregulated the kernel pathways, neuroactive ligand–receptor interaction, and cAMP signaling pathway in the skin tissue homogenate of the diabetic wound healing mice model. In summary, this study identified the potential targets and kernel pathways, providing additional evidence for the clinical application of the SJHY formula for the treatment of diabetic wound healing. |
| format |
article |
| author |
Jing-Si Jiang Jing-Si Jiang Ying Zhang Ying Zhang Ying Luo Ying Luo Yi Ru Yue Luo Xiao-Ya Fei Jian-Kun Song Xiao-Jie Ding Xiao-Jie Ding Zhan Zhang Dan Yang Shuang-Yi Yin Hui-Ping Zhang Tai-Yi Liu Bin Li Bin Li Bin Li Le Kuai Le Kuai |
| author_facet |
Jing-Si Jiang Jing-Si Jiang Ying Zhang Ying Zhang Ying Luo Ying Luo Yi Ru Yue Luo Xiao-Ya Fei Jian-Kun Song Xiao-Jie Ding Xiao-Jie Ding Zhan Zhang Dan Yang Shuang-Yi Yin Hui-Ping Zhang Tai-Yi Liu Bin Li Bin Li Bin Li Le Kuai Le Kuai |
| author_sort |
Jing-Si Jiang |
| title |
The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology |
| title_short |
The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology |
| title_full |
The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology |
| title_fullStr |
The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology |
| title_full_unstemmed |
The Identification of the Biomarkers of Sheng-Ji Hua-Yu Formula Treated Diabetic Wound Healing Using Modular Pharmacology |
| title_sort |
identification of the biomarkers of sheng-ji hua-yu formula treated diabetic wound healing using modular pharmacology |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/5eb3df2495f249ac8f0def9c38b954c7 |
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