Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Ghayathri Balasundaram,1,* Chris Jun Hui Ho,1,* Kai Li,2 Wouter Driessen,3 US Dinish,1 Chi Lok Wong,1 Vasilis Ntziachristos,3 Bin Liu,2 Malini Olivo1,41Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), 2Institute of Materials Research and Engineering (IMRE), Agency for Science, Tec...

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Autores principales:	Balasundaram G, Ho CJH, Li K, Driessen WH, Dinish US, Wong CL, Ntziachristos V, Liu B, Olivo M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5eb55af412e14cb685a6dad6ee53225a
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spelling	oai:doaj.org-article:5eb55af412e14cb685a6dad6ee53225a2021-12-02T05:10:42ZMolecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer1178-2013https://doaj.org/article/5eb55af412e14cb685a6dad6ee53225a2015-01-01T00:00:00Zhttp://www.dovepress.com/molecular-photoacoustic-imaging-of-breast-cancer-using-an-actively-tar-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Ghayathri Balasundaram,1,* Chris Jun Hui Ho,1,* Kai Li,2 Wouter Driessen,3 US Dinish,1 Chi Lok Wong,1 Vasilis Ntziachristos,3 Bin Liu,2 Malini Olivo1,41Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), 2Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (ASTAR), Singapore; 3Institute of Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany; 4School of Physics, National University of Ireland, Galway, Ireland These authors contributed equally to this work Abstract: Conjugated polymers (CPs) are upcoming optical contrast agents in view of their unique optical properties and versatile synthetic chemistry. Biofunctionalization of these polymer-based nanoparticles enables molecular imaging of biological processes. In this work, we propose the concept of using a biofunctionalized CP for noninvasive photoacoustic (PA) molecular imaging of breast cancer. In particular, after verifying the PA activity of a CP nanoparticle (CP dots) in phantoms and the targeting efficacy of a folate-functionalized version of the same (folate-CP dots) in vitro, we systemically administered the probe into a folate receptor-positive (FR+ve) MCF-7 breast cancer xenograft model to demonstrate the possible application of folate-CP dots for imaging FR+ve breast cancers in comparison to CP dots with no folate moieties. We observed a strong PA signal at the tumor site of folate-CP dots-administered mice as early as 1 hour after administration as a result of the active targeting of the folate-CP dots to the FR+ve tumor cells but a weak PA signal at the tumor site of CP-dots-administered mice as a result of the passive accumulation of the probe by enhanced permeability and retention effect. We also observed that folate-CP dots produced ~4-fold enhancement in the PA signal in the tumor, when compared to CP dots. These observations demonstrate the great potential of this active-targeting CP to be used as a contrast agent for molecular PA diagnostic imaging in various biomedical applications. Keywords: photoacoustic tomography, conjugated polymers, molecular imaging, breast cancerBalasundaram GHo CJHLi KDriessen WHDinish USWong CLNtziachristos VLiu BOlivo MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 387-397 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Balasundaram G Ho CJH Li K Driessen WH Dinish US Wong CL Ntziachristos V Liu B Olivo M Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
description	Ghayathri Balasundaram,1,* Chris Jun Hui Ho,1,* Kai Li,2 Wouter Driessen,3 US Dinish,1 Chi Lok Wong,1 Vasilis Ntziachristos,3 Bin Liu,2 Malini Olivo1,41Bio-Optical Imaging Group, Singapore Bioimaging Consortium (SBIC), 2Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (ASTAR), Singapore; 3Institute of Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany; 4School of Physics, National University of Ireland, Galway, Ireland These authors contributed equally to this work Abstract: Conjugated polymers (CPs) are upcoming optical contrast agents in view of their unique optical properties and versatile synthetic chemistry. Biofunctionalization of these polymer-based nanoparticles enables molecular imaging of biological processes. In this work, we propose the concept of using a biofunctionalized CP for noninvasive photoacoustic (PA) molecular imaging of breast cancer. In particular, after verifying the PA activity of a CP nanoparticle (CP dots) in phantoms and the targeting efficacy of a folate-functionalized version of the same (folate-CP dots) in vitro, we systemically administered the probe into a folate receptor-positive (FR+ve) MCF-7 breast cancer xenograft model to demonstrate the possible application of folate-CP dots for imaging FR+ve breast cancers in comparison to CP dots with no folate moieties. We observed a strong PA signal at the tumor site of folate-CP dots-administered mice as early as 1 hour after administration as a result of the active targeting of the folate-CP dots to the FR+ve tumor cells but a weak PA signal at the tumor site of CP-dots-administered mice as a result of the passive accumulation of the probe by enhanced permeability and retention effect. We also observed that folate-CP dots produced ~4-fold enhancement in the PA signal in the tumor, when compared to CP dots. These observations demonstrate the great potential of this active-targeting CP to be used as a contrast agent for molecular PA diagnostic imaging in various biomedical applications. Keywords: photoacoustic tomography, conjugated polymers, molecular imaging, breast cancer
format	article
author	Balasundaram G Ho CJH Li K Driessen WH Dinish US Wong CL Ntziachristos V Liu B Olivo M
author_facet	Balasundaram G Ho CJH Li K Driessen WH Dinish US Wong CL Ntziachristos V Liu B Olivo M
author_sort	Balasundaram G
title	Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
title_short	Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
title_full	Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
title_fullStr	Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
title_full_unstemmed	Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
title_sort	molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/5eb55af412e14cb685a6dad6ee53225a
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Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

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