Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.

Most bacterial strains, which have been studied so far for their probiotic functions, are extensively used by manufacturers in developed countries. In our work, we sought to study a mix (called BSL) comprising three strains belonging to Lactobacillus fermentum, L. paraplantarum and L. salivarius, th...

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Autores principales:	Williams Turpin, Christèle Humblot, Marie-Louise Noordine, Laura Wrzosek, Julie Tomas, Camille Mayeur, Claire Cherbuy, Jean-Pierre Guyot, Muriel Thomas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5eb817db67b14a9294a324685c11f61e
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spelling	oai:doaj.org-article:5eb817db67b14a9294a324685c11f61e2021-11-18T07:50:33ZBehavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.1932-620310.1371/journal.pone.0057711https://doaj.org/article/5eb817db67b14a9294a324685c11f61e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23577056/?tool=EBIhttps://doaj.org/toc/1932-6203Most bacterial strains, which have been studied so far for their probiotic functions, are extensively used by manufacturers in developed countries. In our work, we sought to study a mix (called BSL) comprising three strains belonging to Lactobacillus fermentum, L. paraplantarum and L. salivarius, that were isolated from a traditional African pearl millet based fermented slurry. Our objective was to study this BSL cocktail in gnotobiotic rats, to evaluate their survival and their behavior in the digestive tract conditions. After a single oral inoculation of germfree rats with BSL, the species established stably in the digestive tract with the following hierarchy of abundance: L. salivarius> L. plantarum> L. fermentum. BSL cocktail was metabolically active since it produced 50 mM lactate and it expressed genes involved in binding mechanism in the caecum. Furthermore, the global morphology of the colon epithelium was not disturbed by the BSL cocktail. BSL cocktail did not modify mucus content and host mucus-related genes (MUC1, MUC2, MUC3 or resistin-like molecule β). The cocktail of lactobacilli enhanced the proliferating cell nuclear antigen (PCNA) at a level comparable to what was observed in conventional rats. PCNA was involved in proliferation and DNA repair, but the presence of the cocktail did not provoke proliferative events (with Ki67 as indicator), so we suppose BSL may help gut preservation. This work is the first step towards the selection of strains that are derived from traditional fermented food to formulate new probiotic mixture.Williams TurpinChristèle HumblotMarie-Louise NoordineLaura WrzosekJulie TomasCamille MayeurClaire CherbuyJean-Pierre GuyotMuriel ThomasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e57711 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Williams Turpin Christèle Humblot Marie-Louise Noordine Laura Wrzosek Julie Tomas Camille Mayeur Claire Cherbuy Jean-Pierre Guyot Muriel Thomas Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
description	Most bacterial strains, which have been studied so far for their probiotic functions, are extensively used by manufacturers in developed countries. In our work, we sought to study a mix (called BSL) comprising three strains belonging to Lactobacillus fermentum, L. paraplantarum and L. salivarius, that were isolated from a traditional African pearl millet based fermented slurry. Our objective was to study this BSL cocktail in gnotobiotic rats, to evaluate their survival and their behavior in the digestive tract conditions. After a single oral inoculation of germfree rats with BSL, the species established stably in the digestive tract with the following hierarchy of abundance: L. salivarius> L. plantarum> L. fermentum. BSL cocktail was metabolically active since it produced 50 mM lactate and it expressed genes involved in binding mechanism in the caecum. Furthermore, the global morphology of the colon epithelium was not disturbed by the BSL cocktail. BSL cocktail did not modify mucus content and host mucus-related genes (MUC1, MUC2, MUC3 or resistin-like molecule β). The cocktail of lactobacilli enhanced the proliferating cell nuclear antigen (PCNA) at a level comparable to what was observed in conventional rats. PCNA was involved in proliferation and DNA repair, but the presence of the cocktail did not provoke proliferative events (with Ki67 as indicator), so we suppose BSL may help gut preservation. This work is the first step towards the selection of strains that are derived from traditional fermented food to formulate new probiotic mixture.
format	article
author	Williams Turpin Christèle Humblot Marie-Louise Noordine Laura Wrzosek Julie Tomas Camille Mayeur Claire Cherbuy Jean-Pierre Guyot Muriel Thomas
author_facet	Williams Turpin Christèle Humblot Marie-Louise Noordine Laura Wrzosek Julie Tomas Camille Mayeur Claire Cherbuy Jean-Pierre Guyot Muriel Thomas
author_sort	Williams Turpin
title	Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
title_short	Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
title_full	Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
title_fullStr	Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
title_full_unstemmed	Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
title_sort	behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5eb817db67b14a9294a324685c11f61e
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Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.

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