Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit

Abstract. Sibero MT, Siswanto AP, Frederick EH, Wijaya AP, Syafitri E, Farabi K, Murwani R, Saito S, Igaras Y. 2020. Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit. Biodiversitas 21: 4147-4154. The local community in...

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Autores principales:	Mada Triandala Sibero, ANGGUN PUSPITARINI SISWANTO, RETNO MURWANI, EVAN HANSEL FREDERICK, ALDI PRATAMA WIJAYA, EMMY SYAFITRI, KINDI FARABI, SHUN SAITO, YASUHIRO IGARASHI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	andaliman, cytotoxicity, leukemia, terpenes, zanthoxylum Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5eb908e799ab4a93bf849797eb4ae73f
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spelling	oai:doaj.org-article:5eb908e799ab4a93bf849797eb4ae73f2021-11-22T00:41:43ZAntibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit1412-033X2085-472210.13057/biodiv/d210928https://doaj.org/article/5eb908e799ab4a93bf849797eb4ae73f2020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6464https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sibero MT, Siswanto AP, Frederick EH, Wijaya AP, Syafitri E, Farabi K, Murwani R, Saito S, Igaras Y. 2020. Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit. Biodiversitas 21: 4147-4154. The local community in North Sumatra has utilized andaliman fruit (Zanthoxylum acanthopodium) as spices for traditional cuisines because it has a unique flavor. Information on the antimicrobial activity of Z. acanthopodium fruit against aquaculture pathogens and its bioactivity against leukemia cell lines are limited. The purposes of this study were to evaluate the antimicrobial activity of Z. acanthopodium fruit against Tenacibaculum maritimum, Vibrio alginolyticus, V. anguillarum, V. harveyi that are known as pathogens in aquaculture; to determine cytotoxic property against murine P388 leukemia cells; and to characterize its metabolites profile. The sample was extracted using methanol by the maceration method. Antibacterial assay was conducted by Kirby-Bauer disc diffusion method; while cytotoxicity assay using the XTT method. Proximate analysis showed that Z. acanthopodium fruit contained 63.41% of moisture, 24.73% of crude fiber, 9.81% of crude protein, 6.90% of ash, and 2.55% of crude fat. Several phytochemical components were detected, such as alkaloid, flavonoid, tannin, triterpenoid, and steroid. The GC/MS analysis indicated the presence of various compounds from terpenoid and terpenes derivatives. This study indicated that Z. acanthopodium fruit was not potential as antibacterial agents against the aquaculture pathogens; however, the methanol extract showed cytotoxic potential with IC50 19.14 µg/mL against murine P388 leukemia cells.Mada Triandala SiberoANGGUN PUSPITARINI SISWANTORETNO MURWANIEVAN HANSEL FREDERICKALDI PRATAMA WIJAYAEMMY SYAFITRIKINDI FARABISHUN SAITOYASUHIRO IGARASHIMBI & UNS Soloarticleandaliman, cytotoxicity, leukemia, terpenes, zanthoxylumBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	andaliman, cytotoxicity, leukemia, terpenes, zanthoxylum Biology (General) QH301-705.5
spellingShingle	andaliman, cytotoxicity, leukemia, terpenes, zanthoxylum Biology (General) QH301-705.5 Mada Triandala Sibero ANGGUN PUSPITARINI SISWANTO RETNO MURWANI EVAN HANSEL FREDERICK ALDI PRATAMA WIJAYA EMMY SYAFITRI KINDI FARABI SHUN SAITO YASUHIRO IGARASHI Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit
description	Abstract. Sibero MT, Siswanto AP, Frederick EH, Wijaya AP, Syafitri E, Farabi K, Murwani R, Saito S, Igaras Y. 2020. Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit. Biodiversitas 21: 4147-4154. The local community in North Sumatra has utilized andaliman fruit (Zanthoxylum acanthopodium) as spices for traditional cuisines because it has a unique flavor. Information on the antimicrobial activity of Z. acanthopodium fruit against aquaculture pathogens and its bioactivity against leukemia cell lines are limited. The purposes of this study were to evaluate the antimicrobial activity of Z. acanthopodium fruit against Tenacibaculum maritimum, Vibrio alginolyticus, V. anguillarum, V. harveyi that are known as pathogens in aquaculture; to determine cytotoxic property against murine P388 leukemia cells; and to characterize its metabolites profile. The sample was extracted using methanol by the maceration method. Antibacterial assay was conducted by Kirby-Bauer disc diffusion method; while cytotoxicity assay using the XTT method. Proximate analysis showed that Z. acanthopodium fruit contained 63.41% of moisture, 24.73% of crude fiber, 9.81% of crude protein, 6.90% of ash, and 2.55% of crude fat. Several phytochemical components were detected, such as alkaloid, flavonoid, tannin, triterpenoid, and steroid. The GC/MS analysis indicated the presence of various compounds from terpenoid and terpenes derivatives. This study indicated that Z. acanthopodium fruit was not potential as antibacterial agents against the aquaculture pathogens; however, the methanol extract showed cytotoxic potential with IC50 19.14 µg/mL against murine P388 leukemia cells.
format	article
author	Mada Triandala Sibero ANGGUN PUSPITARINI SISWANTO RETNO MURWANI EVAN HANSEL FREDERICK ALDI PRATAMA WIJAYA EMMY SYAFITRI KINDI FARABI SHUN SAITO YASUHIRO IGARASHI
author_facet	Mada Triandala Sibero ANGGUN PUSPITARINI SISWANTO RETNO MURWANI EVAN HANSEL FREDERICK ALDI PRATAMA WIJAYA EMMY SYAFITRI KINDI FARABI SHUN SAITO YASUHIRO IGARASHI
author_sort	Mada Triandala Sibero
title	Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit
title_short	Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit
title_full	Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit
title_fullStr	Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit
title_full_unstemmed	Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit
title_sort	antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (zanthoxylum acanthopodium) fruit
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/5eb908e799ab4a93bf849797eb4ae73f
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Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit

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