Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.

Myoblasts undergo a series of changes in the composition and dynamics of their plasma membranes during the initial steps of skeletal muscle differentiation. These changes are crucial requirements for myoblast fusion and allow the formation of striated muscle fibers. Membrane microdomains, or lipid r...

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Autores principales: Ana Claudia Batista Possidonio, Carolina Pontes Soares, Débora Morueco Portilho, Victor Midlej, Marlene Benchimol, Gillian Butler-Browne, Manoel Luis Costa, Claudia Mermelstein
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Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/5ebd20c54c304a8a8f56ea7dc0158b81
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spelling oai:doaj.org-article:5ebd20c54c304a8a8f56ea7dc0158b812021-11-25T06:05:33ZDifferences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.1932-620310.1371/journal.pone.0103990https://doaj.org/article/5ebd20c54c304a8a8f56ea7dc0158b812014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25105415/?tool=EBIhttps://doaj.org/toc/1932-6203Myoblasts undergo a series of changes in the composition and dynamics of their plasma membranes during the initial steps of skeletal muscle differentiation. These changes are crucial requirements for myoblast fusion and allow the formation of striated muscle fibers. Membrane microdomains, or lipid rafts, have been implicated in myoblast fusion. Flotillins are scaffold proteins that are essential for the formation and dynamics of lipid rafts. Flotillins have been widely studied over the last few years, but still little is known about their role during skeletal muscle differentiation. In the present study, we analyzed the expression and distribution of flotillin-2 in chick, mice and human muscle cells grown in vitro. Primary cultures of chick myogenic cells showed a decrease in the expression of flotillin-2 during the first 72 hours of muscle differentiation. Interestingly, flotillin-2 was found to be highly expressed in chick myogenic fibroblasts and weakly expressed in chick myoblasts and multinucleated myotubes. Flotillin-2 was distributed in vesicle-like structures within the cytoplasm of chick myogenic fibroblasts, in the mouse C2C12 myogenic cell line, and in neonatal human muscle cells. Cryo-immunogold labeling revealed the presence of flotillin-2 in vesicles and in Golgi stacks in chick myogenic fibroblasts. Further, brefeldin A induced a major reduction in the number of flotillin-2 containing vesicles which correlates to a decrease in myoblast fusion. These results suggest the involvement of flotillin-2 during the initial steps of skeletal myogenesis.Ana Claudia Batista PossidonioCarolina Pontes SoaresDébora Morueco PortilhoVictor MidlejMarlene BenchimolGillian Butler-BrowneManoel Luis CostaClaudia MermelsteinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103990 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ana Claudia Batista Possidonio
Carolina Pontes Soares
Débora Morueco Portilho
Victor Midlej
Marlene Benchimol
Gillian Butler-Browne
Manoel Luis Costa
Claudia Mermelstein
Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
description Myoblasts undergo a series of changes in the composition and dynamics of their plasma membranes during the initial steps of skeletal muscle differentiation. These changes are crucial requirements for myoblast fusion and allow the formation of striated muscle fibers. Membrane microdomains, or lipid rafts, have been implicated in myoblast fusion. Flotillins are scaffold proteins that are essential for the formation and dynamics of lipid rafts. Flotillins have been widely studied over the last few years, but still little is known about their role during skeletal muscle differentiation. In the present study, we analyzed the expression and distribution of flotillin-2 in chick, mice and human muscle cells grown in vitro. Primary cultures of chick myogenic cells showed a decrease in the expression of flotillin-2 during the first 72 hours of muscle differentiation. Interestingly, flotillin-2 was found to be highly expressed in chick myogenic fibroblasts and weakly expressed in chick myoblasts and multinucleated myotubes. Flotillin-2 was distributed in vesicle-like structures within the cytoplasm of chick myogenic fibroblasts, in the mouse C2C12 myogenic cell line, and in neonatal human muscle cells. Cryo-immunogold labeling revealed the presence of flotillin-2 in vesicles and in Golgi stacks in chick myogenic fibroblasts. Further, brefeldin A induced a major reduction in the number of flotillin-2 containing vesicles which correlates to a decrease in myoblast fusion. These results suggest the involvement of flotillin-2 during the initial steps of skeletal myogenesis.
format article
author Ana Claudia Batista Possidonio
Carolina Pontes Soares
Débora Morueco Portilho
Victor Midlej
Marlene Benchimol
Gillian Butler-Browne
Manoel Luis Costa
Claudia Mermelstein
author_facet Ana Claudia Batista Possidonio
Carolina Pontes Soares
Débora Morueco Portilho
Victor Midlej
Marlene Benchimol
Gillian Butler-Browne
Manoel Luis Costa
Claudia Mermelstein
author_sort Ana Claudia Batista Possidonio
title Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
title_short Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
title_full Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
title_fullStr Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
title_full_unstemmed Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
title_sort differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/5ebd20c54c304a8a8f56ea7dc0158b81
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