Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads

Abstract Psychrotrophic Pseudomonas species are the key spoilage bacteria of aerobically stored chilled meat. These organisms readily form biofilms on meat under refrigerated conditions leading to consumer rejection and associated economic losses. Limited information is available on the matrix compo...

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Autores principales:	Nirmani N. Wickramasinghe, Mya M. Hlaing, Joshua T. Ravensdale, Ranil Coorey, P. Scott Chandry, Gary A. Dykes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ec0c8bb4aa145f49f2fe76895ceebbe
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spelling	oai:doaj.org-article:5ec0c8bb4aa145f49f2fe76895ceebbe2021-12-02T18:37:06ZCharacterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads10.1038/s41598-020-73612-02045-2322https://doaj.org/article/5ec0c8bb4aa145f49f2fe76895ceebbe2020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73612-0https://doaj.org/toc/2045-2322Abstract Psychrotrophic Pseudomonas species are the key spoilage bacteria of aerobically stored chilled meat. These organisms readily form biofilms on meat under refrigerated conditions leading to consumer rejection and associated economic losses. Limited information is available on the matrix composition of the biofilms formed by these bacteria. We quantified and characterized the main components of the matrix of mono-species biofilms of selected Pseudomonas fragi and Pseudomonas lundensis strains using chemical analysis and Raman spectroscopy. The biofilms were grown at 10 °C and 25 °C on nitro-cellulose membranes placed on surface sterilized beef cuts. Extra-cellular polymeric substances of the matrix were extracted in soluble and bound forms and were chemically assessed for total carbohydrates, proteins and extra-cellular DNA. Both Pseudomonas species showed a significant increase in total carbohydrates and total proteins when grown at 10 °C as compared to 25 °C. Extra-cellular DNA did not show a strong correlation with growth temperature. Raman spectra were obtained from planktonic bacteria and membrane grown biofilms at 10 °C and 25 °C. Higher levels of guanine were detected in planktonic cells as compared to biofilm cells. This study suggests that psychrotrophic Pseudomonas species may respond to cold stress by increasing extra-cellular polymer secretions.Nirmani N. WickramasingheMya M. HlaingJoshua T. RavensdaleRanil CooreyP. Scott ChandryGary A. DykesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nirmani N. Wickramasinghe Mya M. Hlaing Joshua T. Ravensdale Ranil Coorey P. Scott Chandry Gary A. Dykes Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
description	Abstract Psychrotrophic Pseudomonas species are the key spoilage bacteria of aerobically stored chilled meat. These organisms readily form biofilms on meat under refrigerated conditions leading to consumer rejection and associated economic losses. Limited information is available on the matrix composition of the biofilms formed by these bacteria. We quantified and characterized the main components of the matrix of mono-species biofilms of selected Pseudomonas fragi and Pseudomonas lundensis strains using chemical analysis and Raman spectroscopy. The biofilms were grown at 10 °C and 25 °C on nitro-cellulose membranes placed on surface sterilized beef cuts. Extra-cellular polymeric substances of the matrix were extracted in soluble and bound forms and were chemically assessed for total carbohydrates, proteins and extra-cellular DNA. Both Pseudomonas species showed a significant increase in total carbohydrates and total proteins when grown at 10 °C as compared to 25 °C. Extra-cellular DNA did not show a strong correlation with growth temperature. Raman spectra were obtained from planktonic bacteria and membrane grown biofilms at 10 °C and 25 °C. Higher levels of guanine were detected in planktonic cells as compared to biofilm cells. This study suggests that psychrotrophic Pseudomonas species may respond to cold stress by increasing extra-cellular polymer secretions.
format	article
author	Nirmani N. Wickramasinghe Mya M. Hlaing Joshua T. Ravensdale Ranil Coorey P. Scott Chandry Gary A. Dykes
author_facet	Nirmani N. Wickramasinghe Mya M. Hlaing Joshua T. Ravensdale Ranil Coorey P. Scott Chandry Gary A. Dykes
author_sort	Nirmani N. Wickramasinghe
title	Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
title_short	Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
title_full	Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
title_fullStr	Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
title_full_unstemmed	Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
title_sort	characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5ec0c8bb4aa145f49f2fe76895ceebbe
work_keys_str_mv	AT nirmaninwickramasinghe characterizationofthebiofilmmatrixcompositionofpsychrotrophicmeatspoilagepseudomonads AT myamhlaing characterizationofthebiofilmmatrixcompositionofpsychrotrophicmeatspoilagepseudomonads AT joshuatravensdale characterizationofthebiofilmmatrixcompositionofpsychrotrophicmeatspoilagepseudomonads AT ranilcoorey characterizationofthebiofilmmatrixcompositionofpsychrotrophicmeatspoilagepseudomonads AT pscottchandry characterizationofthebiofilmmatrixcompositionofpsychrotrophicmeatspoilagepseudomonads AT garyadykes characterizationofthebiofilmmatrixcompositionofpsychrotrophicmeatspoilagepseudomonads
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Characterization of the biofilm matrix composition of psychrotrophic, meat spoilage pseudomonads

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