Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.

<h4>Introduction</h4>Recently, electric cigarettes with liquid (e-liquid) were introduced as an alternative to tobacco smoking. They were promoted as possible cessation aids and were considered to be potentially less harmful than traditional tobacco-based cigarettes. However, there is li...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyung Joon Kwon, Young Taek Oh, Saewhan Park, Sung Soo Kim, Jinju Park, Jinlong Yin, Jun Hee Hong, Chan Il Kim, Haseo Ryu, Jong Bae Park, Min Kyung Lim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ee8cd711d3f4ab2b929120f51e710df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5ee8cd711d3f4ab2b929120f51e710df
record_format	dspace
spelling	oai:doaj.org-article:5ee8cd711d3f4ab2b929120f51e710df2021-12-02T20:14:46ZAnalysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.1932-620310.1371/journal.pone.0256730https://doaj.org/article/5ee8cd711d3f4ab2b929120f51e710df2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256730https://doaj.org/toc/1932-6203<h4>Introduction</h4>Recently, electric cigarettes with liquid (e-liquid) were introduced as an alternative to tobacco smoking. They were promoted as possible cessation aids and were considered to be potentially less harmful than traditional tobacco-based cigarettes. However, there is little information on the toxicants present in e-liquids and their possible carcinogenic effects.<h4>Methods</h4>Western blot analysis was performed to identify the protein levels of cancer progression related signal transducers. Patient-derived brain tumor cells (CSC2) were injected into mouse brains and tumor growth was then observed by performing magnetic resonance imaging (MRI) and hematoxylin and eosin (H&E) staining of the whole brain. Immunohistochemistry (IHC) staining and Immunofluorescence staining were performed to study the expression of pEGFR and pERK.<h4>Results</h4>Western blotting revealed that e-liquids increased pEGFR and pERK expression in a dose dependent manner. Animal experiments revealed that the e-liquid treated group had accelerated tumor growth and poor prognosis compared to the vehicle group. Histological staining showed activation of pEGFR and pERK in the e-liquid treated group.<h4>Conclusion</h4>Our study revealed that e-liquid activates pEGFR and pERK, leading to accelerated brain tumor growth and poor prognosis.Hyung Joon KwonYoung Taek OhSaewhan ParkSung Soo KimJinju ParkJinlong YinJun Hee HongChan Il KimHaseo RyuJong Bae ParkMin Kyung LimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256730 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyung Joon Kwon Young Taek Oh Saewhan Park Sung Soo Kim Jinju Park Jinlong Yin Jun Hee Hong Chan Il Kim Haseo Ryu Jong Bae Park Min Kyung Lim Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
description	<h4>Introduction</h4>Recently, electric cigarettes with liquid (e-liquid) were introduced as an alternative to tobacco smoking. They were promoted as possible cessation aids and were considered to be potentially less harmful than traditional tobacco-based cigarettes. However, there is little information on the toxicants present in e-liquids and their possible carcinogenic effects.<h4>Methods</h4>Western blot analysis was performed to identify the protein levels of cancer progression related signal transducers. Patient-derived brain tumor cells (CSC2) were injected into mouse brains and tumor growth was then observed by performing magnetic resonance imaging (MRI) and hematoxylin and eosin (H&E) staining of the whole brain. Immunohistochemistry (IHC) staining and Immunofluorescence staining were performed to study the expression of pEGFR and pERK.<h4>Results</h4>Western blotting revealed that e-liquids increased pEGFR and pERK expression in a dose dependent manner. Animal experiments revealed that the e-liquid treated group had accelerated tumor growth and poor prognosis compared to the vehicle group. Histological staining showed activation of pEGFR and pERK in the e-liquid treated group.<h4>Conclusion</h4>Our study revealed that e-liquid activates pEGFR and pERK, leading to accelerated brain tumor growth and poor prognosis.
format	article
author	Hyung Joon Kwon Young Taek Oh Saewhan Park Sung Soo Kim Jinju Park Jinlong Yin Jun Hee Hong Chan Il Kim Haseo Ryu Jong Bae Park Min Kyung Lim
author_facet	Hyung Joon Kwon Young Taek Oh Saewhan Park Sung Soo Kim Jinju Park Jinlong Yin Jun Hee Hong Chan Il Kim Haseo Ryu Jong Bae Park Min Kyung Lim
author_sort	Hyung Joon Kwon
title	Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
title_short	Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
title_full	Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
title_fullStr	Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
title_full_unstemmed	Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.
title_sort	analysis of electric cigarette liquid effect on mouse brain tumor growth through egfr and erk activation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5ee8cd711d3f4ab2b929120f51e710df
work_keys_str_mv	AT hyungjoonkwon analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT youngtaekoh analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT saewhanpark analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT sungsookim analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT jinjupark analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT jinlongyin analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT junheehong analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT chanilkim analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT haseoryu analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT jongbaepark analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation AT minkyunglim analysisofelectriccigaretteliquideffectonmousebraintumorgrowththroughegfranderkactivation
_version_	1718374647270998016

Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.

Ejemplares similares