Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents

Abstract Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role...

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Autores principales:	Khaled Abdallah, Francis Nadeau, Francis Bergeron, Sylvie Blouin, Véronique Blais, Kelly M. Bradbury, Christine L. Lavoie, Jean-Luc Parent, Louis Gendron
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5eeb6fec159e4ba2ad1fe5c9dd194b56
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spelling	oai:doaj.org-article:5eeb6fec159e4ba2ad1fe5c9dd194b562021-12-02T15:07:44ZAdeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents10.1038/s41598-018-25626-y2045-2322https://doaj.org/article/5eeb6fec159e4ba2ad1fe5c9dd194b562018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25626-yhttps://doaj.org/toc/2045-2322Abstract Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role of specific peptides and proteins in a given population of cells. In the present study, we describe an efficient approach to selectively deliver a Cre-GFP to dorsal root ganglia (DRG) neurons. First, mice of different ages were injected in both hindpaws with a recombinant adeno-associated virus (rAAV2/9-CBA-Cre-GFP). Using this route of injection in mice at 5 days of age, we report that approximately 20% of all DRG neurons express GFP, 6 to 8 weeks after the infection. The level of infection was reduced by 50% when the virus was administered at 2 weeks of age. Additionally, the virus-mediated delivery of the Cre-GFP was also investigated via the intrathecal route. When injected intrathecally, the rAAV2/9-CBA-Cre-GFP virus infected a much higher proportion of DRG neurons than the intraplantar injection, with up to 51.6% of infected lumbar DRG neurons. Noteworthy, both routes of injection predominantly transduced DRG neurons over spinal and brain neurons.Khaled AbdallahFrancis NadeauFrancis BergeronSylvie BlouinVéronique BlaisKelly M. BradburyChristine L. LavoieJean-Luc ParentLouis GendronNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khaled Abdallah Francis Nadeau Francis Bergeron Sylvie Blouin Véronique Blais Kelly M. Bradbury Christine L. Lavoie Jean-Luc Parent Louis Gendron Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
description	Abstract Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role of specific peptides and proteins in a given population of cells. In the present study, we describe an efficient approach to selectively deliver a Cre-GFP to dorsal root ganglia (DRG) neurons. First, mice of different ages were injected in both hindpaws with a recombinant adeno-associated virus (rAAV2/9-CBA-Cre-GFP). Using this route of injection in mice at 5 days of age, we report that approximately 20% of all DRG neurons express GFP, 6 to 8 weeks after the infection. The level of infection was reduced by 50% when the virus was administered at 2 weeks of age. Additionally, the virus-mediated delivery of the Cre-GFP was also investigated via the intrathecal route. When injected intrathecally, the rAAV2/9-CBA-Cre-GFP virus infected a much higher proportion of DRG neurons than the intraplantar injection, with up to 51.6% of infected lumbar DRG neurons. Noteworthy, both routes of injection predominantly transduced DRG neurons over spinal and brain neurons.
format	article
author	Khaled Abdallah Francis Nadeau Francis Bergeron Sylvie Blouin Véronique Blais Kelly M. Bradbury Christine L. Lavoie Jean-Luc Parent Louis Gendron
author_facet	Khaled Abdallah Francis Nadeau Francis Bergeron Sylvie Blouin Véronique Blais Kelly M. Bradbury Christine L. Lavoie Jean-Luc Parent Louis Gendron
author_sort	Khaled Abdallah
title	Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_short	Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_full	Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_fullStr	Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_full_unstemmed	Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_sort	adeno-associated virus 2/9 delivery of cre recombinase in mouse primary afferents
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5eeb6fec159e4ba2ad1fe5c9dd194b56
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Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents

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