Global mapping of miRNA-target interactions in cattle (Bos taurus)

Abstract With roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential therapeutic target. Here we used cross-linking immunoprecipitation (CLIP) and ligation of miRNA-target chimeras on the Argonaute (AGO) protein to globally map miRNA in...

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Autores principales:	Troels K. H. Scheel, Michael J. Moore, Joseph M. Luna, Eiko Nishiuchi, John Fak, Robert B. Darnell, Charles M. Rice
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5eec22a589664124912d9a0ed35d92b7
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spelling	oai:doaj.org-article:5eec22a589664124912d9a0ed35d92b72021-12-02T15:05:10ZGlobal mapping of miRNA-target interactions in cattle (Bos taurus)10.1038/s41598-017-07880-82045-2322https://doaj.org/article/5eec22a589664124912d9a0ed35d92b72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07880-8https://doaj.org/toc/2045-2322Abstract With roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential therapeutic target. Here we used cross-linking immunoprecipitation (CLIP) and ligation of miRNA-target chimeras on the Argonaute (AGO) protein to globally map miRNA interactions in the cow. The interactome is the deepest reported to date. miRNA targeting principles are consistent with observations in other species, but with expanded pairing rules. Experimental mapping robustly predicted functional miR-17 regulatory sites. From miRNA-specific targeting for >5000 mRNAs we determined gene ontologies (GO). This confirmed repression of genes important for embryonic development and cell cycle progress by the let-7 family, and repression of those involved in cell cycle arrest by the miR-17 family, but also suggested a number of unappreciated miRNA functions. Our results provide a significant resource for understanding of bovine and species-conserved miRNA regulation, and demonstrate the power of experimental methods for establishing comprehensive interaction maps.Troels K. H. ScheelMichael J. MooreJoseph M. LunaEiko NishiuchiJohn FakRobert B. DarnellCharles M. RiceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Troels K. H. Scheel Michael J. Moore Joseph M. Luna Eiko Nishiuchi John Fak Robert B. Darnell Charles M. Rice Global mapping of miRNA-target interactions in cattle (Bos taurus)
description	Abstract With roles in development, cell proliferation and disease, micro-RNA (miRNA) biology is of great importance and a potential therapeutic target. Here we used cross-linking immunoprecipitation (CLIP) and ligation of miRNA-target chimeras on the Argonaute (AGO) protein to globally map miRNA interactions in the cow. The interactome is the deepest reported to date. miRNA targeting principles are consistent with observations in other species, but with expanded pairing rules. Experimental mapping robustly predicted functional miR-17 regulatory sites. From miRNA-specific targeting for >5000 mRNAs we determined gene ontologies (GO). This confirmed repression of genes important for embryonic development and cell cycle progress by the let-7 family, and repression of those involved in cell cycle arrest by the miR-17 family, but also suggested a number of unappreciated miRNA functions. Our results provide a significant resource for understanding of bovine and species-conserved miRNA regulation, and demonstrate the power of experimental methods for establishing comprehensive interaction maps.
format	article
author	Troels K. H. Scheel Michael J. Moore Joseph M. Luna Eiko Nishiuchi John Fak Robert B. Darnell Charles M. Rice
author_facet	Troels K. H. Scheel Michael J. Moore Joseph M. Luna Eiko Nishiuchi John Fak Robert B. Darnell Charles M. Rice
author_sort	Troels K. H. Scheel
title	Global mapping of miRNA-target interactions in cattle (Bos taurus)
title_short	Global mapping of miRNA-target interactions in cattle (Bos taurus)
title_full	Global mapping of miRNA-target interactions in cattle (Bos taurus)
title_fullStr	Global mapping of miRNA-target interactions in cattle (Bos taurus)
title_full_unstemmed	Global mapping of miRNA-target interactions in cattle (Bos taurus)
title_sort	global mapping of mirna-target interactions in cattle (bos taurus)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5eec22a589664124912d9a0ed35d92b7
work_keys_str_mv	AT troelskhscheel globalmappingofmirnatargetinteractionsincattlebostaurus AT michaeljmoore globalmappingofmirnatargetinteractionsincattlebostaurus AT josephmluna globalmappingofmirnatargetinteractionsincattlebostaurus AT eikonishiuchi globalmappingofmirnatargetinteractionsincattlebostaurus AT johnfak globalmappingofmirnatargetinteractionsincattlebostaurus AT robertbdarnell globalmappingofmirnatargetinteractionsincattlebostaurus AT charlesmrice globalmappingofmirnatargetinteractionsincattlebostaurus
_version_	1718388892197978112

Global mapping of miRNA-target interactions in cattle (Bos taurus)

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