Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.

As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpres...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yong Zhou, Li Liu, Weifeng Huang, Meng Yuan, Fei Zhou, Xianghua Li, Yongjun Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5eec3f7256a3487b9f60a1b21c0b952f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5eec3f7256a3487b9f60a1b21c0b952f
record_format	dspace
spelling	oai:doaj.org-article:5eec3f7256a3487b9f60a1b21c0b952f2021-11-18T08:24:43ZOverexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.1932-620310.1371/journal.pone.0094210https://doaj.org/article/5eec3f7256a3487b9f60a1b21c0b952f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24709840/?tool=EBIhttps://doaj.org/toc/1932-6203As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpressing plants displayed the phenotypes of growth retardation and precocious senescence at seedling stage. GC-MS analysis showed that the sugar levels were largely altered in the leaves of the OsSWEET5-overexpressing plants. Molecular analysis revealed that these phenotypes might be due to the transcriptional changes of the genes involved in sugar metabolism and transport. In addition, the transgenic plants showed a lower level of auxin with altered transcription of genes involved in auxin signaling and translocation pathways. However, no obvious phenotype was observed between the amiRNA-OsSWEET5 transgenic lines and WT plants, which could be a result of the functional redundancy of the galactose transporters in rice. Taken together, our findings suggest that OsSWEET5 plays a crucial role in regulating the crosstalk between sugar and auxin in rice.Yong ZhouLi LiuWeifeng HuangMeng YuanFei ZhouXianghua LiYongjun LinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94210 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yong Zhou Li Liu Weifeng Huang Meng Yuan Fei Zhou Xianghua Li Yongjun Lin Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
description	As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpressing plants displayed the phenotypes of growth retardation and precocious senescence at seedling stage. GC-MS analysis showed that the sugar levels were largely altered in the leaves of the OsSWEET5-overexpressing plants. Molecular analysis revealed that these phenotypes might be due to the transcriptional changes of the genes involved in sugar metabolism and transport. In addition, the transgenic plants showed a lower level of auxin with altered transcription of genes involved in auxin signaling and translocation pathways. However, no obvious phenotype was observed between the amiRNA-OsSWEET5 transgenic lines and WT plants, which could be a result of the functional redundancy of the galactose transporters in rice. Taken together, our findings suggest that OsSWEET5 plays a crucial role in regulating the crosstalk between sugar and auxin in rice.
format	article
author	Yong Zhou Li Liu Weifeng Huang Meng Yuan Fei Zhou Xianghua Li Yongjun Lin
author_facet	Yong Zhou Li Liu Weifeng Huang Meng Yuan Fei Zhou Xianghua Li Yongjun Lin
author_sort	Yong Zhou
title	Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
title_short	Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
title_full	Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
title_fullStr	Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
title_full_unstemmed	Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.
title_sort	overexpression of ossweet5 in rice causes growth retardation and precocious senescence.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5eec3f7256a3487b9f60a1b21c0b952f
work_keys_str_mv	AT yongzhou overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT liliu overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT weifenghuang overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT mengyuan overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT feizhou overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT xianghuali overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence AT yongjunlin overexpressionofossweet5inricecausesgrowthretardationandprecocioussenescence
_version_	1718421803797315584

Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence.

Ejemplares similares