Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Plant genome editing typically relies upon transgenic intermediates, which is a concern given the current regulatory requirements concerning GMOs. Here, Zhang et al. describe a method to edit wheat genomes by transiently expressing CRISPR/Cas9 DNA or RNA, and are able to generate mutant plants with...

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Autores principales:	Yi Zhang, Zhen Liang, Yuan Zong, Yanpeng Wang, Jinxing Liu, Kunling Chen, Jin-Long Qiu, Caixia Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5eeff47d0d90427daddfe48074ff7432
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spelling	oai:doaj.org-article:5eeff47d0d90427daddfe48074ff74322021-12-02T16:49:26ZEfficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA10.1038/ncomms126172041-1723https://doaj.org/article/5eeff47d0d90427daddfe48074ff74322016-08-01T00:00:00Zhttps://doi.org/10.1038/ncomms12617https://doaj.org/toc/2041-1723Plant genome editing typically relies upon transgenic intermediates, which is a concern given the current regulatory requirements concerning GMOs. Here, Zhang et al. describe a method to edit wheat genomes by transiently expressing CRISPR/Cas9 DNA or RNA, and are able to generate mutant plants with no detectable transgenes.Yi ZhangZhen LiangYuan ZongYanpeng WangJinxing LiuKunling ChenJin-Long QiuCaixia GaoNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yi Zhang Zhen Liang Yuan Zong Yanpeng Wang Jinxing Liu Kunling Chen Jin-Long Qiu Caixia Gao Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
description	Plant genome editing typically relies upon transgenic intermediates, which is a concern given the current regulatory requirements concerning GMOs. Here, Zhang et al. describe a method to edit wheat genomes by transiently expressing CRISPR/Cas9 DNA or RNA, and are able to generate mutant plants with no detectable transgenes.
format	article
author	Yi Zhang Zhen Liang Yuan Zong Yanpeng Wang Jinxing Liu Kunling Chen Jin-Long Qiu Caixia Gao
author_facet	Yi Zhang Zhen Liang Yuan Zong Yanpeng Wang Jinxing Liu Kunling Chen Jin-Long Qiu Caixia Gao
author_sort	Yi Zhang
title	Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
title_short	Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
title_full	Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
title_fullStr	Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
title_full_unstemmed	Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
title_sort	efficient and transgene-free genome editing in wheat through transient expression of crispr/cas9 dna or rna
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/5eeff47d0d90427daddfe48074ff7432
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Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

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