In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.

The cells of the mononuclear phagocyte system are essential for the correct healing of adult skin wounds, but their specific functions remain ill-defined. The absence of granulation tissue immediately after skin injury makes it challenging to study the role of mononuclear phagocytes at the initiatio...

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Autores principales:	Mathieu P Rodero, Fabrice Licata, Lucie Poupel, Pauline Hamon, Kiarash Khosrotehrani, Christophe Combadiere, Alexandre Boissonnas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ef6ee61fc364368a13f40c9ca9a3bad
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spelling	oai:doaj.org-article:5ef6ee61fc364368a13f40c9ca9a3bad2021-11-25T05:58:18ZIn vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.1932-620310.1371/journal.pone.0108212https://doaj.org/article/5ef6ee61fc364368a13f40c9ca9a3bad2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108212https://doaj.org/toc/1932-6203The cells of the mononuclear phagocyte system are essential for the correct healing of adult skin wounds, but their specific functions remain ill-defined. The absence of granulation tissue immediately after skin injury makes it challenging to study the role of mononuclear phagocytes at the initiation of this inflammatory stage. To study their recruitment and migratory behavior within the wound bed, we developed a new model for real-time in vivo imaging of the wound, using transgenic mice that express green and cyan fluorescent proteins and specifically target monocytes. Within hours after the scalp injury, monocytes invaded the wound bed. The complete abrogation of this infiltration in monocyte-deficient CCR2(-/-) mice argues for the involvement of classical monocytes in this process. Monocyte infiltration unexpectedly occurred as early as neutrophil recruitment did and resulted from active release from the bloodstream toward the matrix through microhemorrhages rather than transendothelial migration. Monocytes randomly scouted around the wound bed, progressively slowed down, and stopped. Our approach identified and characterized a rapid and earlier than expected wave of monocyte infiltration and provides a novel framework for investigating the role of these cells during early stages of wound healing.Mathieu P RoderoFabrice LicataLucie PoupelPauline HamonKiarash KhosrotehraniChristophe CombadiereAlexandre BoissonnasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e108212 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathieu P Rodero Fabrice Licata Lucie Poupel Pauline Hamon Kiarash Khosrotehrani Christophe Combadiere Alexandre Boissonnas In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
description	The cells of the mononuclear phagocyte system are essential for the correct healing of adult skin wounds, but their specific functions remain ill-defined. The absence of granulation tissue immediately after skin injury makes it challenging to study the role of mononuclear phagocytes at the initiation of this inflammatory stage. To study their recruitment and migratory behavior within the wound bed, we developed a new model for real-time in vivo imaging of the wound, using transgenic mice that express green and cyan fluorescent proteins and specifically target monocytes. Within hours after the scalp injury, monocytes invaded the wound bed. The complete abrogation of this infiltration in monocyte-deficient CCR2(-/-) mice argues for the involvement of classical monocytes in this process. Monocyte infiltration unexpectedly occurred as early as neutrophil recruitment did and resulted from active release from the bloodstream toward the matrix through microhemorrhages rather than transendothelial migration. Monocytes randomly scouted around the wound bed, progressively slowed down, and stopped. Our approach identified and characterized a rapid and earlier than expected wave of monocyte infiltration and provides a novel framework for investigating the role of these cells during early stages of wound healing.
format	article
author	Mathieu P Rodero Fabrice Licata Lucie Poupel Pauline Hamon Kiarash Khosrotehrani Christophe Combadiere Alexandre Boissonnas
author_facet	Mathieu P Rodero Fabrice Licata Lucie Poupel Pauline Hamon Kiarash Khosrotehrani Christophe Combadiere Alexandre Boissonnas
author_sort	Mathieu P Rodero
title	In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
title_short	In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
title_full	In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
title_fullStr	In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
title_full_unstemmed	In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
title_sort	in vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5ef6ee61fc364368a13f40c9ca9a3bad
work_keys_str_mv	AT mathieuprodero invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds AT fabricelicata invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds AT luciepoupel invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds AT paulinehamon invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds AT kiarashkhosrotehrani invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds AT christophecombadiere invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds AT alexandreboissonnas invivoimagingrevealsapioneerwaveofmonocyterecruitmentintomouseskinwounds
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In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds.

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