Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells

Abstract In our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we inves...

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Autores principales:	Myoung Hwa Kim, Jeong-Rim Lee, Ki-Joon Kim, Ji Hae Jun, Hye Jeong Hwang, Wootaek Lee, Seung Hyun Nam, Ju Eun Oh, Young Chul Yoo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5ef8925d4f0049b1933526862b0fc7d1
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spelling	oai:doaj.org-article:5ef8925d4f0049b1933526862b0fc7d12021-11-14T12:17:53ZIdentification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells10.1038/s41598-021-01701-92045-2322https://doaj.org/article/5ef8925d4f0049b1933526862b0fc7d12021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01701-9https://doaj.org/toc/2045-2322Abstract In our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the anti-tumor effect of tramadol in vivo. Female nude mice orthotopically inoculated with luciferase-expressing MCF-7 cells, were randomly divided into the control (saline), tramadol group 1 (1.5 mg kg−1 day−1), tramadol group 2 (3 mg kg−1 day−1), and morphine (0.5 mg kg−1 day−1) (n = 5/group). Bioluminescence signals after D-luciferin injection, tumor size, and tumor weight were compared among groups after 4 weeks. Estrogen receptor (ER), progesterone receptor (PR), and transient receptor potential vanilloid (TRPV)-1 expression, natural killer (NK) cell activity, and serum interleukin (IL)-1β, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-6 were then examined. Tumour growth was attenuated in tramadol-treated groups (P < 0.05). NK cell activity was significantly decreased only in the morphine treated group not in sham, control, and tramadol groups. The expression levels of ERα, PRα and β, and TRPV1 were decreased in tramadol group 2 compared with those in the morphine group, but not compared to the control group. Serum levels of IL-6 and TNFα were reduced in both tramadol-treated group 1 and 2 compared to the control group. Overall, clinical dose of tramadol has anti-tumour effects on MCF-7 cell-derived breast cancer in a xenograft mouse model.Myoung Hwa KimJeong-Rim LeeKi-Joon KimJi Hae JunHye Jeong HwangWootaek LeeSeung Hyun NamJu Eun OhYoung Chul YooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Myoung Hwa Kim Jeong-Rim Lee Ki-Joon Kim Ji Hae Jun Hye Jeong Hwang Wootaek Lee Seung Hyun Nam Ju Eun Oh Young Chul Yoo Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
description	Abstract In our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the anti-tumor effect of tramadol in vivo. Female nude mice orthotopically inoculated with luciferase-expressing MCF-7 cells, were randomly divided into the control (saline), tramadol group 1 (1.5 mg kg−1 day−1), tramadol group 2 (3 mg kg−1 day−1), and morphine (0.5 mg kg−1 day−1) (n = 5/group). Bioluminescence signals after D-luciferin injection, tumor size, and tumor weight were compared among groups after 4 weeks. Estrogen receptor (ER), progesterone receptor (PR), and transient receptor potential vanilloid (TRPV)-1 expression, natural killer (NK) cell activity, and serum interleukin (IL)-1β, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-6 were then examined. Tumour growth was attenuated in tramadol-treated groups (P < 0.05). NK cell activity was significantly decreased only in the morphine treated group not in sham, control, and tramadol groups. The expression levels of ERα, PRα and β, and TRPV1 were decreased in tramadol group 2 compared with those in the morphine group, but not compared to the control group. Serum levels of IL-6 and TNFα were reduced in both tramadol-treated group 1 and 2 compared to the control group. Overall, clinical dose of tramadol has anti-tumour effects on MCF-7 cell-derived breast cancer in a xenograft mouse model.
format	article
author	Myoung Hwa Kim Jeong-Rim Lee Ki-Joon Kim Ji Hae Jun Hye Jeong Hwang Wootaek Lee Seung Hyun Nam Ju Eun Oh Young Chul Yoo
author_facet	Myoung Hwa Kim Jeong-Rim Lee Ki-Joon Kim Ji Hae Jun Hye Jeong Hwang Wootaek Lee Seung Hyun Nam Ju Eun Oh Young Chul Yoo
author_sort	Myoung Hwa Kim
title	Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
title_short	Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
title_full	Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
title_fullStr	Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
title_full_unstemmed	Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
title_sort	identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5ef8925d4f0049b1933526862b0fc7d1
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Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells

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