MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer.
To elucidate the function of MAS-related GPCR, member D (MRGD) in cancers, we investigated the in vitro and in vivo oncogenic function of MRGD using murine fibroblast cell line NIH3T3 in which MRGD is stably expressed. The expression pattern of MRGD in clinical samples was also analyzed. We found th...
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2012
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oai:doaj.org-article:5ef89b6b48ae48b18795906bda91ef6e2021-11-18T07:15:56ZMRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer.1932-620310.1371/journal.pone.0038618https://doaj.org/article/5ef89b6b48ae48b18795906bda91ef6e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22715397/?tool=EBIhttps://doaj.org/toc/1932-6203To elucidate the function of MAS-related GPCR, member D (MRGD) in cancers, we investigated the in vitro and in vivo oncogenic function of MRGD using murine fibroblast cell line NIH3T3 in which MRGD is stably expressed. The expression pattern of MRGD in clinical samples was also analyzed. We found that overexpression of MRGD in NIH3T3 induced focus formation and multi-cellular spheroid formation, and promoted tumors in nude mice. In other words, overexpression of MRGD in NIH3T3 induced the loss of contact inhibition, anchorage-independent growth and in vivo tumorigenesis. Furthermore, it was found that the ligand of MRGD, beta-alanine, enhanced spheroid formation in MRGD-expressing NIH3T3 cells. From investigation of clinical cancer tissues, we found high expression of MRGD in several lung cancers by immunohistochemistry as well as real time PCR. Based on these results, MRGD could be involved in tumorigenesis and could also be a novel anticancer drug target.Satoko NishimuraMakiko UnoYasuyuki KanetaKeisuke FukuchiHaruyuki NishigohriJun HasegawaHironobu KomoriShigeki TakedaKatsuhiko EnomotoFutoshi NaraToshinori AgatsumaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38618 (2012) |
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Medicine R Science Q |
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Medicine R Science Q Satoko Nishimura Makiko Uno Yasuyuki Kaneta Keisuke Fukuchi Haruyuki Nishigohri Jun Hasegawa Hironobu Komori Shigeki Takeda Katsuhiko Enomoto Futoshi Nara Toshinori Agatsuma MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| description |
To elucidate the function of MAS-related GPCR, member D (MRGD) in cancers, we investigated the in vitro and in vivo oncogenic function of MRGD using murine fibroblast cell line NIH3T3 in which MRGD is stably expressed. The expression pattern of MRGD in clinical samples was also analyzed. We found that overexpression of MRGD in NIH3T3 induced focus formation and multi-cellular spheroid formation, and promoted tumors in nude mice. In other words, overexpression of MRGD in NIH3T3 induced the loss of contact inhibition, anchorage-independent growth and in vivo tumorigenesis. Furthermore, it was found that the ligand of MRGD, beta-alanine, enhanced spheroid formation in MRGD-expressing NIH3T3 cells. From investigation of clinical cancer tissues, we found high expression of MRGD in several lung cancers by immunohistochemistry as well as real time PCR. Based on these results, MRGD could be involved in tumorigenesis and could also be a novel anticancer drug target. |
| format |
article |
| author |
Satoko Nishimura Makiko Uno Yasuyuki Kaneta Keisuke Fukuchi Haruyuki Nishigohri Jun Hasegawa Hironobu Komori Shigeki Takeda Katsuhiko Enomoto Futoshi Nara Toshinori Agatsuma |
| author_facet |
Satoko Nishimura Makiko Uno Yasuyuki Kaneta Keisuke Fukuchi Haruyuki Nishigohri Jun Hasegawa Hironobu Komori Shigeki Takeda Katsuhiko Enomoto Futoshi Nara Toshinori Agatsuma |
| author_sort |
Satoko Nishimura |
| title |
MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| title_short |
MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| title_full |
MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| title_fullStr |
MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| title_full_unstemmed |
MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| title_sort |
mrgd, a mas-related g-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/5ef89b6b48ae48b18795906bda91ef6e |
| work_keys_str_mv |
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