Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension

Arterial stiffness due to the vessel remodeling is closely linked to raised blood pressure, and its physiopathologic mechanism is still not fully understood. We here aimed to explore whether extracellular vesicle (EV) mediated intercellular communication between endothelium and smooth muscle cell co...

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Autores principales:	Chen Wang, Changyang Xing, Zhelong Li, Yunnan Liu, Qiaoying Li, Yixiao Wang, Jiao Hu, Lijun Yuan, Guodong Yang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Hypertension Extracellular vesicles Endothelial cell Smooth muscle cell Phenotype switch microRNA Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5eff8fcd88404295b32a5578e2751198
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spelling	oai:doaj.org-article:5eff8fcd88404295b32a5578e27511982021-11-04T04:34:59ZBioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension2452-199X10.1016/j.bioactmat.2021.06.005https://doaj.org/article/5eff8fcd88404295b32a5578e27511982022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21002887https://doaj.org/toc/2452-199XArterial stiffness due to the vessel remodeling is closely linked to raised blood pressure, and its physiopathologic mechanism is still not fully understood. We here aimed to explore whether extracellular vesicle (EV) mediated intercellular communication between endothelium and smooth muscle cell contribute to the blood vessel remodeling under hypertension. We here revealed that the arterial endothelial cells robustly secreted EV, which in turn could be circulated and/or directly taken up by the subendothelial smooth muscle cells (SMC). Under hypertension, the EV secretion increased and the miRNA profile changed significantly mainly due to the raised mechanical force and subsequent enhanced reactive oxygen species generation. Among the miRNA cargos in the EV, miR-320d/423-5p were found increased most significantly. In vivo delivery of miR-320d/423-5p mimics via engineered EV increased their expression in arterial vessels, recapitulating the phenotype in hypertension. In contrast, therapeutic delivery of miR-320d/423-5p inhibitors via engineered EV alleviated the phenotype in spontaneous hypertension rat model. Together, we have found that the injured endothelium due to the raised mechanical force in hypertension contributes to the arterial wall remodeling via the secreted EV. Our study has not only provided novel insights on the mechanism of hypertension associated blood vessel wall remodeling, but also shed light on therapeutic intervention of hypertension associated vascular diseases.Chen WangChangyang XingZhelong LiYunnan LiuQiaoying LiYixiao WangJiao HuLijun YuanGuodong YangKeAi Communications Co., Ltd.articleHypertensionExtracellular vesiclesEndothelial cellSmooth muscle cellPhenotype switchmicroRNAMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 494-504 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hypertension Extracellular vesicles Endothelial cell Smooth muscle cell Phenotype switch microRNA Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Hypertension Extracellular vesicles Endothelial cell Smooth muscle cell Phenotype switch microRNA Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Chen Wang Changyang Xing Zhelong Li Yunnan Liu Qiaoying Li Yixiao Wang Jiao Hu Lijun Yuan Guodong Yang Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
description	Arterial stiffness due to the vessel remodeling is closely linked to raised blood pressure, and its physiopathologic mechanism is still not fully understood. We here aimed to explore whether extracellular vesicle (EV) mediated intercellular communication between endothelium and smooth muscle cell contribute to the blood vessel remodeling under hypertension. We here revealed that the arterial endothelial cells robustly secreted EV, which in turn could be circulated and/or directly taken up by the subendothelial smooth muscle cells (SMC). Under hypertension, the EV secretion increased and the miRNA profile changed significantly mainly due to the raised mechanical force and subsequent enhanced reactive oxygen species generation. Among the miRNA cargos in the EV, miR-320d/423-5p were found increased most significantly. In vivo delivery of miR-320d/423-5p mimics via engineered EV increased their expression in arterial vessels, recapitulating the phenotype in hypertension. In contrast, therapeutic delivery of miR-320d/423-5p inhibitors via engineered EV alleviated the phenotype in spontaneous hypertension rat model. Together, we have found that the injured endothelium due to the raised mechanical force in hypertension contributes to the arterial wall remodeling via the secreted EV. Our study has not only provided novel insights on the mechanism of hypertension associated blood vessel wall remodeling, but also shed light on therapeutic intervention of hypertension associated vascular diseases.
format	article
author	Chen Wang Changyang Xing Zhelong Li Yunnan Liu Qiaoying Li Yixiao Wang Jiao Hu Lijun Yuan Guodong Yang
author_facet	Chen Wang Changyang Xing Zhelong Li Yunnan Liu Qiaoying Li Yixiao Wang Jiao Hu Lijun Yuan Guodong Yang
author_sort	Chen Wang
title	Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
title_short	Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
title_full	Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
title_fullStr	Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
title_full_unstemmed	Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
title_sort	bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/5eff8fcd88404295b32a5578e2751198
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_version_	1718445274870841344

Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension

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