Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing

Summary: Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized ti...

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Autores principales:	Pierfrancesco Pagella, Bernd Stadlinger, Thimios A. Mitsiadis
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell isolation Single Cell Health Sciences Genomics Stem Cells Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/5f01691802804519a6e10b2223ef16c5
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spelling	oai:doaj.org-article:5f01691802804519a6e10b2223ef16c52021-11-18T04:51:40ZIsolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing2666-166710.1016/j.xpro.2021.100953https://doaj.org/article/5f01691802804519a6e10b2223ef16c52021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006596https://doaj.org/toc/2666-1667Summary: Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized tissues (dentin, cementum, and alveolar bone). Here we describe a protocol to isolate all cells composing human dental pulp and periodontium for single-cell RNA sequencing analysis.For complete details on the use and execution of this protocol, please refer to Pagella et al. (2021).Pierfrancesco PagellaBernd StadlingerThimios A. MitsiadisElsevierarticleCell BiologyCell isolationSingle CellHealth SciencesGenomicsStem CellsScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100953- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell isolation Single Cell Health Sciences Genomics Stem Cells Science (General) Q1-390
spellingShingle	Cell Biology Cell isolation Single Cell Health Sciences Genomics Stem Cells Science (General) Q1-390 Pierfrancesco Pagella Bernd Stadlinger Thimios A. Mitsiadis Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
description	Summary: Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized tissues (dentin, cementum, and alveolar bone). Here we describe a protocol to isolate all cells composing human dental pulp and periodontium for single-cell RNA sequencing analysis.For complete details on the use and execution of this protocol, please refer to Pagella et al. (2021).
format	article
author	Pierfrancesco Pagella Bernd Stadlinger Thimios A. Mitsiadis
author_facet	Pierfrancesco Pagella Bernd Stadlinger Thimios A. Mitsiadis
author_sort	Pierfrancesco Pagella
title	Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_short	Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_full	Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_fullStr	Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_full_unstemmed	Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_sort	isolation of dental pulp and periodontal cells from human teeth for single-cell rna sequencing
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5f01691802804519a6e10b2223ef16c5
work_keys_str_mv	AT pierfrancescopagella isolationofdentalpulpandperiodontalcellsfromhumanteethforsinglecellrnasequencing AT berndstadlinger isolationofdentalpulpandperiodontalcellsfromhumanteethforsinglecellrnasequencing AT thimiosamitsiadis isolationofdentalpulpandperiodontalcellsfromhumanteethforsinglecellrnasequencing
_version_	1718424976379346944

Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing

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