Incorporation of sensing modalities into de novo designed fluorescence-activating proteins

Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with increased photostability and smaller size, which bind...

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Autores principales:	Jason C. Klima, Lindsey A. Doyle, Justin Daho Lee, Michael Rappleye, Lauren A. Gagnon, Min Yen Lee, Emilia P. Barros, Anastassia A. Vorobieva, Jiayi Dou, Samantha Bremner, Jacob S. Quon, Cameron M. Chow, Lauren Carter, David L. Mack, Rommie E. Amaro, Joshua C. Vaughan, Andre Berndt, Barry L. Stoddard, David Baker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
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spelling	oai:doaj.org-article:5f1531b0f2b14a3183076ec5a9a3c8592021-12-02T14:26:44ZIncorporation of sensing modalities into de novo designed fluorescence-activating proteins10.1038/s41467-020-18911-w2041-1723https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c8592021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18911-whttps://doaj.org/toc/2041-1723Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with increased photostability and smaller size, which bind a range of DFHBI chromophore variants.Jason C. KlimaLindsey A. DoyleJustin Daho LeeMichael RappleyeLauren A. GagnonMin Yen LeeEmilia P. BarrosAnastassia A. VorobievaJiayi DouSamantha BremnerJacob S. QuonCameron M. ChowLauren CarterDavid L. MackRommie E. AmaroJoshua C. VaughanAndre BerndtBarry L. StoddardDavid BakerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jason C. Klima Lindsey A. Doyle Justin Daho Lee Michael Rappleye Lauren A. Gagnon Min Yen Lee Emilia P. Barros Anastassia A. Vorobieva Jiayi Dou Samantha Bremner Jacob S. Quon Cameron M. Chow Lauren Carter David L. Mack Rommie E. Amaro Joshua C. Vaughan Andre Berndt Barry L. Stoddard David Baker Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
description	Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with increased photostability and smaller size, which bind a range of DFHBI chromophore variants.
format	article
author	Jason C. Klima Lindsey A. Doyle Justin Daho Lee Michael Rappleye Lauren A. Gagnon Min Yen Lee Emilia P. Barros Anastassia A. Vorobieva Jiayi Dou Samantha Bremner Jacob S. Quon Cameron M. Chow Lauren Carter David L. Mack Rommie E. Amaro Joshua C. Vaughan Andre Berndt Barry L. Stoddard David Baker
author_facet	Jason C. Klima Lindsey A. Doyle Justin Daho Lee Michael Rappleye Lauren A. Gagnon Min Yen Lee Emilia P. Barros Anastassia A. Vorobieva Jiayi Dou Samantha Bremner Jacob S. Quon Cameron M. Chow Lauren Carter David L. Mack Rommie E. Amaro Joshua C. Vaughan Andre Berndt Barry L. Stoddard David Baker
author_sort	Jason C. Klima
title	Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
title_short	Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
title_full	Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
title_fullStr	Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
title_full_unstemmed	Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
title_sort	incorporation of sensing modalities into de novo designed fluorescence-activating proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
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Incorporation of sensing modalities into de novo designed fluorescence-activating proteins

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