A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.

A model betalainic dye was semisynthesized from betanin, the magenta pigment of the red beet, and was effective for live-cell imaging of Plasmodium-infected red blood cells. This water-soluble fluorescent probe is photostable, excitable in the visible region and cell membrane-permeable, and its phot...

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Autores principales:	Letícia Christina Pires Gonçalves, Renata Rosito Tonelli, Piero Bagnaresi, Renato Arruda Mortara, Antonio Gilberto Ferreira, Erick Leite Bastos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f19b1d4ee1f4f248f6158c21c914bc0
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spelling	oai:doaj.org-article:5f19b1d4ee1f4f248f6158c21c914bc02021-11-18T08:01:14ZA nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.1932-620310.1371/journal.pone.0053874https://doaj.org/article/5f19b1d4ee1f4f248f6158c21c914bc02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23342028/?tool=EBIhttps://doaj.org/toc/1932-6203A model betalainic dye was semisynthesized from betanin, the magenta pigment of the red beet, and was effective for live-cell imaging of Plasmodium-infected red blood cells. This water-soluble fluorescent probe is photostable, excitable in the visible region and cell membrane-permeable, and its photophysical properties are not notably pH-sensitive. Fluorescence imaging microscopy of erythrocytes infected with Plasmodium falciparum, a causative agent of malaria in humans, showed that only the parasite was stained. Z-stacking analysis suggested that the probe accumulates proximal to the nucleus of the parasite. Indicaxanthin, one of the natural fluorescent betalains found in the petals of certain flowers, did not stain the parasite or the red blood cell.Letícia Christina Pires GonçalvesRenata Rosito TonelliPiero BagnaresiRenato Arruda MortaraAntonio Gilberto FerreiraErick Leite BastosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53874 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Letícia Christina Pires Gonçalves Renata Rosito Tonelli Piero Bagnaresi Renato Arruda Mortara Antonio Gilberto Ferreira Erick Leite Bastos A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.
description	A model betalainic dye was semisynthesized from betanin, the magenta pigment of the red beet, and was effective for live-cell imaging of Plasmodium-infected red blood cells. This water-soluble fluorescent probe is photostable, excitable in the visible region and cell membrane-permeable, and its photophysical properties are not notably pH-sensitive. Fluorescence imaging microscopy of erythrocytes infected with Plasmodium falciparum, a causative agent of malaria in humans, showed that only the parasite was stained. Z-stacking analysis suggested that the probe accumulates proximal to the nucleus of the parasite. Indicaxanthin, one of the natural fluorescent betalains found in the petals of certain flowers, did not stain the parasite or the red blood cell.
format	article
author	Letícia Christina Pires Gonçalves Renata Rosito Tonelli Piero Bagnaresi Renato Arruda Mortara Antonio Gilberto Ferreira Erick Leite Bastos
author_facet	Letícia Christina Pires Gonçalves Renata Rosito Tonelli Piero Bagnaresi Renato Arruda Mortara Antonio Gilberto Ferreira Erick Leite Bastos
author_sort	Letícia Christina Pires Gonçalves
title	A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.
title_short	A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.
title_full	A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.
title_fullStr	A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.
title_full_unstemmed	A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.
title_sort	nature-inspired betalainic probe for live-cell imaging of plasmodium-infected erythrocytes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5f19b1d4ee1f4f248f6158c21c914bc0
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A nature-inspired betalainic probe for live-cell imaging of Plasmodium-infected erythrocytes.

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