Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.

<h4>Introduction</h4>Pancreatic cancer (PCA) is an aggressive tumor that associates with high mortality rates. Majority of PCA patients are diagnosed usually at late tumor stages when the therapeutic options are limited. MicroRNAs (miRNA) are involved in tumor development and are commonl...

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Autores principales:	Alexander Link, Verena Becker, Ajay Goel, Thomas Wex, Peter Malfertheiner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f1b5d5613ec42fd927b81931aed673d
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spelling	oai:doaj.org-article:5f1b5d5613ec42fd927b81931aed673d2021-11-18T07:09:16ZFeasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.1932-620310.1371/journal.pone.0042933https://doaj.org/article/5f1b5d5613ec42fd927b81931aed673d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905187/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>Pancreatic cancer (PCA) is an aggressive tumor that associates with high mortality rates. Majority of PCA patients are diagnosed usually at late tumor stages when the therapeutic options are limited. MicroRNAs (miRNA) are involved in tumor development and are commonly dysregulated in PCA. As a proof-of-principle study, we aimed to evaluate the potential of fecal miRNAs as biomarkers for pancreatic cancer.<h4>Materials and methods</h4>Total RNA was extracted from feces using Qiagen's miRNA Mini Kit. For miRNA expression analyses we selected a subset of 7 miRNAs that are frequently dysregulated in PCA (miR-21, -143, -155, -196a, -210, -216a, -375). Subsequently, expression levels of these miRNAs were determined in fecal samples from controls (n = 15), chronic pancreatitis (n = 15) and PCA patients (n = 15) using quantitative TaqMan-PCR assays.<h4>Results</h4>All selected miRNAs were detectable in fecal samples with high reproducibility. Four of seven miRNAs (miR-216a, -196a, -143 und -155) were detected at lower concentrations in feces of PCA patients when compared to controls (p<0.05). Analysis of fecal miRNA expression in controls and patients with chronic pancreatitis and PCA revealed that the expression of miR-216a, -196a, -143 und -155 were highest in controls and lowest in PCA. The expression of the remaining three miRNAs (miR-21, -210 and -375) remained unchanged among controls and the patients with either chronic pancreatitis or PCA.<h4>Conclusion</h4>Our data provide novel evidence for the differential expression of miRNAs in feces of patients with PCA. If successfully validated in large-scale prospective studies, the fecal miRNA biomarkers may offer novel tools for PCA screening research.Alexander LinkVerena BeckerAjay GoelThomas WexPeter MalfertheinerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42933 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander Link Verena Becker Ajay Goel Thomas Wex Peter Malfertheiner Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.
description	<h4>Introduction</h4>Pancreatic cancer (PCA) is an aggressive tumor that associates with high mortality rates. Majority of PCA patients are diagnosed usually at late tumor stages when the therapeutic options are limited. MicroRNAs (miRNA) are involved in tumor development and are commonly dysregulated in PCA. As a proof-of-principle study, we aimed to evaluate the potential of fecal miRNAs as biomarkers for pancreatic cancer.<h4>Materials and methods</h4>Total RNA was extracted from feces using Qiagen's miRNA Mini Kit. For miRNA expression analyses we selected a subset of 7 miRNAs that are frequently dysregulated in PCA (miR-21, -143, -155, -196a, -210, -216a, -375). Subsequently, expression levels of these miRNAs were determined in fecal samples from controls (n = 15), chronic pancreatitis (n = 15) and PCA patients (n = 15) using quantitative TaqMan-PCR assays.<h4>Results</h4>All selected miRNAs were detectable in fecal samples with high reproducibility. Four of seven miRNAs (miR-216a, -196a, -143 und -155) were detected at lower concentrations in feces of PCA patients when compared to controls (p<0.05). Analysis of fecal miRNA expression in controls and patients with chronic pancreatitis and PCA revealed that the expression of miR-216a, -196a, -143 und -155 were highest in controls and lowest in PCA. The expression of the remaining three miRNAs (miR-21, -210 and -375) remained unchanged among controls and the patients with either chronic pancreatitis or PCA.<h4>Conclusion</h4>Our data provide novel evidence for the differential expression of miRNAs in feces of patients with PCA. If successfully validated in large-scale prospective studies, the fecal miRNA biomarkers may offer novel tools for PCA screening research.
format	article
author	Alexander Link Verena Becker Ajay Goel Thomas Wex Peter Malfertheiner
author_facet	Alexander Link Verena Becker Ajay Goel Thomas Wex Peter Malfertheiner
author_sort	Alexander Link
title	Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.
title_short	Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.
title_full	Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.
title_fullStr	Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.
title_full_unstemmed	Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.
title_sort	feasibility of fecal micrornas as novel biomarkers for pancreatic cancer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5f1b5d5613ec42fd927b81931aed673d
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Feasibility of fecal microRNAs as novel biomarkers for pancreatic cancer.

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