Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.

Cerebral malaria (CM) and severe anemia (SA) are the most severe complications of Plasmodium falciparum infections. Although increased release of endothelial microparticles (MP) correlates with malaria severity, the full extent of vascular cell vesiculation remains unknown. Here, we characterize the...

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Autores principales:	Joël Bertrand Pankoui Mfonkeu, Inocent Gouado, Honoré Fotso Kuaté, Odile Zambou, Paul Henri Amvam Zollo, Georges Emile Raymond Grau, Valéry Combes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f1b796122de4dd6b21abe1fe99b73a2
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spelling	oai:doaj.org-article:5f1b796122de4dd6b21abe1fe99b73a22021-11-18T07:03:18ZElevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.1932-620310.1371/journal.pone.0013415https://doaj.org/article/5f1b796122de4dd6b21abe1fe99b73a22010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976232/?tool=EBIhttps://doaj.org/toc/1932-6203Cerebral malaria (CM) and severe anemia (SA) are the most severe complications of Plasmodium falciparum infections. Although increased release of endothelial microparticles (MP) correlates with malaria severity, the full extent of vascular cell vesiculation remains unknown. Here, we characterize the pattern of cell-specific MP in patients with severe malaria. We tested the hypothesis that systemic vascular activation contributes to CM by examining origins and levels of plasma MP in relation to clinical syndromes, disease severity and outcome. Patients recruited in Douala, Cameroon, were assigned to clinical groups following WHO criteria. MP quantitation and phenotyping were carried out using cell-specific markers by flow cytometry using antibodies recognizing cell-specific surface markers. Platelet, erythrocytic, endothelial and leukocytic MP levels were elevated in patients with cerebral dysfunctions and returned to normal by discharge. In CM patients, platelet MP were the most abundant and their levels significantly correlated with coma depth and thrombocytopenia. This study shows for the first time a widespread enhancement of vesiculation in the vascular compartment appears to be a feature of CM but not of SA. Our data underpin the role of MP as a biomarker of neurological involvement in severe malaria. Therefore, intervention to block MP production in severe malaria may provide a new therapeutic pathway.Joël Bertrand Pankoui MfonkeuInocent GouadoHonoré Fotso KuatéOdile ZambouPaul Henri Amvam ZolloGeorges Emile Raymond GrauValéry CombesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13415 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joël Bertrand Pankoui Mfonkeu Inocent Gouado Honoré Fotso Kuaté Odile Zambou Paul Henri Amvam Zollo Georges Emile Raymond Grau Valéry Combes Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
description	Cerebral malaria (CM) and severe anemia (SA) are the most severe complications of Plasmodium falciparum infections. Although increased release of endothelial microparticles (MP) correlates with malaria severity, the full extent of vascular cell vesiculation remains unknown. Here, we characterize the pattern of cell-specific MP in patients with severe malaria. We tested the hypothesis that systemic vascular activation contributes to CM by examining origins and levels of plasma MP in relation to clinical syndromes, disease severity and outcome. Patients recruited in Douala, Cameroon, were assigned to clinical groups following WHO criteria. MP quantitation and phenotyping were carried out using cell-specific markers by flow cytometry using antibodies recognizing cell-specific surface markers. Platelet, erythrocytic, endothelial and leukocytic MP levels were elevated in patients with cerebral dysfunctions and returned to normal by discharge. In CM patients, platelet MP were the most abundant and their levels significantly correlated with coma depth and thrombocytopenia. This study shows for the first time a widespread enhancement of vesiculation in the vascular compartment appears to be a feature of CM but not of SA. Our data underpin the role of MP as a biomarker of neurological involvement in severe malaria. Therefore, intervention to block MP production in severe malaria may provide a new therapeutic pathway.
format	article
author	Joël Bertrand Pankoui Mfonkeu Inocent Gouado Honoré Fotso Kuaté Odile Zambou Paul Henri Amvam Zollo Georges Emile Raymond Grau Valéry Combes
author_facet	Joël Bertrand Pankoui Mfonkeu Inocent Gouado Honoré Fotso Kuaté Odile Zambou Paul Henri Amvam Zollo Georges Emile Raymond Grau Valéry Combes
author_sort	Joël Bertrand Pankoui Mfonkeu
title	Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
title_short	Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
title_full	Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
title_fullStr	Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
title_full_unstemmed	Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
title_sort	elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/5f1b796122de4dd6b21abe1fe99b73a2
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Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria.

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