Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea
Abstract In recent tracheal tissue engineering, limitations in cartilage reconstruction, caused by immature delivery of chondrocyte-laden components, have been reported beyond the complete epithelialization and integration of the tracheal substitutes with the host tissue. In an attempt to overcome s...
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Nature Portfolio
2021
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oai:doaj.org-article:5f1beac3dda149d5ad940179e5a65bc92021-12-02T17:39:20ZImproved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea10.1038/s41598-021-88830-32045-2322https://doaj.org/article/5f1beac3dda149d5ad940179e5a65bc92021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88830-3https://doaj.org/toc/2045-2322Abstract In recent tracheal tissue engineering, limitations in cartilage reconstruction, caused by immature delivery of chondrocyte-laden components, have been reported beyond the complete epithelialization and integration of the tracheal substitutes with the host tissue. In an attempt to overcome such limitations, this article introduces a protective design of tissue-engineered trachea (TraCHIM) composed of a chitosan-based nanofiber membrane (CHIM) and a 3D-printed biotracheal construct. The CHIM was created from chitosan and polycaprolactone (PCL) using an electrospinning process. Upon addition of chitosan to PCL, the diameter of electrospun fibers became thinner, allowing them to be stacked more closely, thereby improving its mechanical properties. Chitosan also enhances the hydrophilicity of the membranes, preventing them from slipping and delaminating over the cell-laden bioink of the biotracheal graft, as well as protecting the construct. Two weeks after implantation in Sprague–Dawley male rats, the group with the TraCHIM exhibited a higher number of chondrocytes, with enhanced chondrogenic performance, than the control group without the membrane. This study successfully demonstrates enhanced chondrogenic performance of TraCHIM in vivo. The protective design of TraCHIM opens a new avenue in engineered tissue research, which requires faster tissue formation from 3D biodegradable materials, to achieve complete replacement of diseased tissue.Hyeonji KimJae Yeon LeeHyeonseok HanWon-Woo ChoHohyeon HanAndrew ChoiHyeonjun HongJae Yun KimJeong Hun ParkSun Hwa ParkSung Won KimDong Sung KimDong-Woo ChoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Hyeonji Kim Jae Yeon Lee Hyeonseok Han Won-Woo Cho Hohyeon Han Andrew Choi Hyeonjun Hong Jae Yun Kim Jeong Hun Park Sun Hwa Park Sung Won Kim Dong Sung Kim Dong-Woo Cho Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea |
| description |
Abstract In recent tracheal tissue engineering, limitations in cartilage reconstruction, caused by immature delivery of chondrocyte-laden components, have been reported beyond the complete epithelialization and integration of the tracheal substitutes with the host tissue. In an attempt to overcome such limitations, this article introduces a protective design of tissue-engineered trachea (TraCHIM) composed of a chitosan-based nanofiber membrane (CHIM) and a 3D-printed biotracheal construct. The CHIM was created from chitosan and polycaprolactone (PCL) using an electrospinning process. Upon addition of chitosan to PCL, the diameter of electrospun fibers became thinner, allowing them to be stacked more closely, thereby improving its mechanical properties. Chitosan also enhances the hydrophilicity of the membranes, preventing them from slipping and delaminating over the cell-laden bioink of the biotracheal graft, as well as protecting the construct. Two weeks after implantation in Sprague–Dawley male rats, the group with the TraCHIM exhibited a higher number of chondrocytes, with enhanced chondrogenic performance, than the control group without the membrane. This study successfully demonstrates enhanced chondrogenic performance of TraCHIM in vivo. The protective design of TraCHIM opens a new avenue in engineered tissue research, which requires faster tissue formation from 3D biodegradable materials, to achieve complete replacement of diseased tissue. |
| format |
article |
| author |
Hyeonji Kim Jae Yeon Lee Hyeonseok Han Won-Woo Cho Hohyeon Han Andrew Choi Hyeonjun Hong Jae Yun Kim Jeong Hun Park Sun Hwa Park Sung Won Kim Dong Sung Kim Dong-Woo Cho |
| author_facet |
Hyeonji Kim Jae Yeon Lee Hyeonseok Han Won-Woo Cho Hohyeon Han Andrew Choi Hyeonjun Hong Jae Yun Kim Jeong Hun Park Sun Hwa Park Sung Won Kim Dong Sung Kim Dong-Woo Cho |
| author_sort |
Hyeonji Kim |
| title |
Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea |
| title_short |
Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea |
| title_full |
Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea |
| title_fullStr |
Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea |
| title_full_unstemmed |
Improved chondrogenic performance with protective tracheal design of Chitosan membrane surrounding 3D-printed trachea |
| title_sort |
improved chondrogenic performance with protective tracheal design of chitosan membrane surrounding 3d-printed trachea |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/5f1beac3dda149d5ad940179e5a65bc9 |
| work_keys_str_mv |
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