The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle

Giora Z Feuerstein,1 Michael A Mansfield,2 Peter I Lelkes,3 Salvatore Alesci,1 Cezary Marcinkiewicz,1,3 Nathan Butlin,4 Mark Sternberg1 1Debina Diagnostics Inc., Newtown Square, PA, USA; 2MilliporeSigma, Bedford, MA, USA; 3Department of Bioengineering, Temple University, Philadelphia, PA, USA; 4Axxi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Feuerstein GZ, Mansfield MA, Lelkes PI, Alesci S, Marcinkiewicz C, Butlin N, Sternberg M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	ebola virus diagnostic lateral flow test (lfa) opto-electronic reader (oer) anti-ebov antibodies nitrocellulose membranes fluidics technology fluorescent nanodiamond particles near infrared light Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5f1ceb1138874f4fac84544c11da4a42
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5f1ceb1138874f4fac84544c11da4a42
record_format	dspace
spelling	oai:doaj.org-article:5f1ceb1138874f4fac84544c11da4a422021-12-02T15:41:07ZThe Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle1178-2013https://doaj.org/article/5f1ceb1138874f4fac84544c11da4a422020-10-01T00:00:00Zhttps://www.dovepress.com/the-use-of-near-infrared-light-emitting-fluorescent-nanodiamond-partic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Giora Z Feuerstein,1 Michael A Mansfield,2 Peter I Lelkes,3 Salvatore Alesci,1 Cezary Marcinkiewicz,1,3 Nathan Butlin,4 Mark Sternberg1 1Debina Diagnostics Inc., Newtown Square, PA, USA; 2MilliporeSigma, Bedford, MA, USA; 3Department of Bioengineering, Temple University, Philadelphia, PA, USA; 4Axxin Pty Ltd, Fairfield, VIC, AustraliaCorrespondence: Giora Z FeuersteinDebina Diagnostics Inc., Newtown Square, PA, USATel +1 4842221575Email cmarcik@temple.eduBackground: There is a dire need for rapid diagnostic tests of high sensitivity, efficiency, and point-of-test reporting capability to mitigate lethal viral epidemic outbreaks.Purpose: To develop a new operating system within the lateral flow assay (LFA) format for Ebola virus (EBOV), based on fluorescent nanodiamond particles (FNDP) nitrogen vacancy (NV) emitting near-infrared (NIR) light. Specifically, we aimed to detail technical issues and the feasibility of mobilizing FNDP-NV on nitrocellulose membranes (NCM) and capturing them at test and control lines.Methods: FNDP-NV-200nm, 400nm or 800nm were linked to anti-EBOV glycoprotein (GP) monoclonal antibodies (mAb) and tested for LFA performance by monitoring NIR emissions using an in vivo imaging system or optoelectronic device (OED). Anti-EBOV recombinant glycoprotein (GP) humanized mAb c13C6 was linked to FNDP-NV-200nm for the mobile phase; and a second anti-GP mouse mAb, 6D8, was printed on NCM at the test line. Goat anti-human IgG (GAH-IgG) served as a nonspecific antibody for conjugated FNDP-NV-200nm at the control line.Results: FNDP-NV-200nm-c13C6 specifically and dose-dependently bound to recombinant EBOV GP in vitro and was effectively captured in a sandwich configuration at the test line by mAb 6D8. FNDP-NV-200nm-c13C6 was captured on the control line by GAH-IgG. The OED quantitative analysis of NIR (obtained in less than 1 minute) was further validated by an in vivo imaging system.Conclusion: FNDP-NV-200nm performance as a reporter for EBOV GP rapid diagnostic tests suggests an opportunity to replace contemporary visual tests for EBOV GP and other highly lethal viral pathogens. Mobile, battery-operated OED adds portability, quantitative data, rapid data collection, and point-of-test reporting capability. Further development of FNDP-NV-200nm within a LFA format is justified.Keywords: Ebola virus, diagnostic lateral flow test, LFA, opto-electronic reader, OER, anti-EBOV antibodies, nitrocellulose membranes, fluidics technologyFeuerstein GZMansfield MALelkes PIAlesci SMarcinkiewicz CButlin NSternberg MDove Medical Pressarticleebola virusdiagnostic lateral flow test (lfa)opto-electronic reader (oer)anti-ebov antibodiesnitrocellulose membranesfluidics technologyfluorescent nanodiamond particlesnear infrared lightMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7583-7599 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ebola virus diagnostic lateral flow test (lfa) opto-electronic reader (oer) anti-ebov antibodies nitrocellulose membranes fluidics technology fluorescent nanodiamond particles near infrared light Medicine (General) R5-920
spellingShingle	ebola virus diagnostic lateral flow test (lfa) opto-electronic reader (oer) anti-ebov antibodies nitrocellulose membranes fluidics technology fluorescent nanodiamond particles near infrared light Medicine (General) R5-920 Feuerstein GZ Mansfield MA Lelkes PI Alesci S Marcinkiewicz C Butlin N Sternberg M The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle
description	Giora Z Feuerstein,1 Michael A Mansfield,2 Peter I Lelkes,3 Salvatore Alesci,1 Cezary Marcinkiewicz,1,3 Nathan Butlin,4 Mark Sternberg1 1Debina Diagnostics Inc., Newtown Square, PA, USA; 2MilliporeSigma, Bedford, MA, USA; 3Department of Bioengineering, Temple University, Philadelphia, PA, USA; 4Axxin Pty Ltd, Fairfield, VIC, AustraliaCorrespondence: Giora Z FeuersteinDebina Diagnostics Inc., Newtown Square, PA, USATel +1 4842221575Email cmarcik@temple.eduBackground: There is a dire need for rapid diagnostic tests of high sensitivity, efficiency, and point-of-test reporting capability to mitigate lethal viral epidemic outbreaks.Purpose: To develop a new operating system within the lateral flow assay (LFA) format for Ebola virus (EBOV), based on fluorescent nanodiamond particles (FNDP) nitrogen vacancy (NV) emitting near-infrared (NIR) light. Specifically, we aimed to detail technical issues and the feasibility of mobilizing FNDP-NV on nitrocellulose membranes (NCM) and capturing them at test and control lines.Methods: FNDP-NV-200nm, 400nm or 800nm were linked to anti-EBOV glycoprotein (GP) monoclonal antibodies (mAb) and tested for LFA performance by monitoring NIR emissions using an in vivo imaging system or optoelectronic device (OED). Anti-EBOV recombinant glycoprotein (GP) humanized mAb c13C6 was linked to FNDP-NV-200nm for the mobile phase; and a second anti-GP mouse mAb, 6D8, was printed on NCM at the test line. Goat anti-human IgG (GAH-IgG) served as a nonspecific antibody for conjugated FNDP-NV-200nm at the control line.Results: FNDP-NV-200nm-c13C6 specifically and dose-dependently bound to recombinant EBOV GP in vitro and was effectively captured in a sandwich configuration at the test line by mAb 6D8. FNDP-NV-200nm-c13C6 was captured on the control line by GAH-IgG. The OED quantitative analysis of NIR (obtained in less than 1 minute) was further validated by an in vivo imaging system.Conclusion: FNDP-NV-200nm performance as a reporter for EBOV GP rapid diagnostic tests suggests an opportunity to replace contemporary visual tests for EBOV GP and other highly lethal viral pathogens. Mobile, battery-operated OED adds portability, quantitative data, rapid data collection, and point-of-test reporting capability. Further development of FNDP-NV-200nm within a LFA format is justified.Keywords: Ebola virus, diagnostic lateral flow test, LFA, opto-electronic reader, OER, anti-EBOV antibodies, nitrocellulose membranes, fluidics technology
format	article
author	Feuerstein GZ Mansfield MA Lelkes PI Alesci S Marcinkiewicz C Butlin N Sternberg M
author_facet	Feuerstein GZ Mansfield MA Lelkes PI Alesci S Marcinkiewicz C Butlin N Sternberg M
author_sort	Feuerstein GZ
title	The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle
title_short	The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle
title_full	The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle
title_fullStr	The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle
title_full_unstemmed	The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle
title_sort	use of near-infrared light-emitting fluorescent nanodiamond particles to detect ebola virus glycoprotein: technology development and proof of principle
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/5f1ceb1138874f4fac84544c11da4a42
work_keys_str_mv	AT feuersteingz theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT mansfieldma theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT lelkespi theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT alescis theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT marcinkiewiczc theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT butlinn theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT sternbergm theuseofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT feuersteingz useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT mansfieldma useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT lelkespi useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT alescis useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT marcinkiewiczc useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT butlinn useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple AT sternbergm useofnearinfraredlightemittingfluorescentnanodiamondparticlestodetectebolavirusglycoproteintechnologydevelopmentandproofofprinciple
_version_	1718385875220430848

The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle

Ejemplares similares