Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions

Abstract Potato virus Y (PVY) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.To explore the molecular PVY-host interactions, we analysed changes in the miRNA and mRNA profiles of tobacc...

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Autores principales:	Yushuang Guo, Meng-ao Jia, Yumei Yang, Linlin Zhan, Xiaofei Cheng, Jianyu Cai, Jie Zhang, Jie Yang, Tao Liu, Qiang Fu, Jiehong Zhao, Imran Haider Shamsi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f2241aa90f54bed9c3ba5a89176dd95
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spelling	oai:doaj.org-article:5f2241aa90f54bed9c3ba5a89176dd952021-12-02T16:06:15ZIntegrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions10.1038/s41598-017-05155-w2045-2322https://doaj.org/article/5f2241aa90f54bed9c3ba5a89176dd952017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05155-whttps://doaj.org/toc/2045-2322Abstract Potato virus Y (PVY) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.To explore the molecular PVY-host interactions, we analysed changes in the miRNA and mRNA profiles of tobacco in response to PVY infection. A total of 81 differentially expressed miRNAs belonging to 29 families and 8133 mRNAs were identified. The Gene Ontology (GO) enrichment analyses showed that genes encoding the DNA/RNA binding, catalytic activity and signalling molecules were all significantly enriched. Moreover, 88 miRNA-mRNA interaction pairs were identified through a combined analysis of the two datasets. We also found evidence showing that the virus-derived siRNAs (vsiRNAs) from the PVY genome target tobacco translationally controlled tumor protein (NtTCTP) mRNA and mediate plant resistance to PVY. Together, our findings revealed that both miRNA and mRNA expression patterns can be changed in response to PVY infection and novel vsiRNA-plant interactions that may regulate plant resistance to PVY. Both provide fresh insights into the virus-plant interactions.Yushuang GuoMeng-ao JiaYumei YangLinlin ZhanXiaofei ChengJianyu CaiJie ZhangJie YangTao LiuQiang FuJiehong ZhaoImran Haider ShamsiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yushuang Guo Meng-ao Jia Yumei Yang Linlin Zhan Xiaofei Cheng Jianyu Cai Jie Zhang Jie Yang Tao Liu Qiang Fu Jiehong Zhao Imran Haider Shamsi Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions
description	Abstract Potato virus Y (PVY) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.To explore the molecular PVY-host interactions, we analysed changes in the miRNA and mRNA profiles of tobacco in response to PVY infection. A total of 81 differentially expressed miRNAs belonging to 29 families and 8133 mRNAs were identified. The Gene Ontology (GO) enrichment analyses showed that genes encoding the DNA/RNA binding, catalytic activity and signalling molecules were all significantly enriched. Moreover, 88 miRNA-mRNA interaction pairs were identified through a combined analysis of the two datasets. We also found evidence showing that the virus-derived siRNAs (vsiRNAs) from the PVY genome target tobacco translationally controlled tumor protein (NtTCTP) mRNA and mediate plant resistance to PVY. Together, our findings revealed that both miRNA and mRNA expression patterns can be changed in response to PVY infection and novel vsiRNA-plant interactions that may regulate plant resistance to PVY. Both provide fresh insights into the virus-plant interactions.
format	article
author	Yushuang Guo Meng-ao Jia Yumei Yang Linlin Zhan Xiaofei Cheng Jianyu Cai Jie Zhang Jie Yang Tao Liu Qiang Fu Jiehong Zhao Imran Haider Shamsi
author_facet	Yushuang Guo Meng-ao Jia Yumei Yang Linlin Zhan Xiaofei Cheng Jianyu Cai Jie Zhang Jie Yang Tao Liu Qiang Fu Jiehong Zhao Imran Haider Shamsi
author_sort	Yushuang Guo
title	Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions
title_short	Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions
title_full	Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions
title_fullStr	Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions
title_full_unstemmed	Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions
title_sort	integrated analysis of tobacco mirna and mrna expression profiles under pvy infection provids insight into tobacco-pvy interactions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5f2241aa90f54bed9c3ba5a89176dd95
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Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions

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