Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization

The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accel...

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Autores principales:	Ming Zhang, Xudong Shi, Hai Sun, Donghua Xu, Yang Gao, Xi Wu, Jianqi Zhang, Jichang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	glycogen synthase kinase-3β inhibitor (GSKi) polydopamine 316L stainless steel endothelialization human coronary artery endothelial cells Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5f242408e0114735afa8216c6d99819b
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spelling	oai:doaj.org-article:5f242408e0114735afa8216c6d99819b2021-11-22T09:11:56ZImmobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization2296-418510.3389/fbioe.2021.806151https://doaj.org/article/5f242408e0114735afa8216c6d99819b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.806151/fullhttps://doaj.org/toc/2296-4185The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accelerate vascular endothelialization. In this work, GSKi was covalently conjugated on 316L stainless steel through polydopamine to develop a stable bioactive surface. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact angle results revealed the successful introduction of GSKi onto 316L stainless steel. The GSKi coating did not obviously affect the hemocompatibility of plates. The adhesion and proliferation of human coronary artery endothelial cells (HCAECs) on stainless steel was significantly promoted by the addition of GSKi. In summary, this work provides a universal and stable strategy of immobilizing GSKi on the stent surface. This method has the potential for widespread application in the modification of vascular stents.Ming ZhangXudong ShiXudong ShiHai SunHai SunDonghua XuYang GaoXi WuJianqi ZhangJichang ZhangFrontiers Media S.A.articleglycogen synthase kinase-3β inhibitor (GSKi)polydopamine316L stainless steelendothelializationhuman coronary artery endothelial cellsBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glycogen synthase kinase-3β inhibitor (GSKi) polydopamine 316L stainless steel endothelialization human coronary artery endothelial cells Biotechnology TP248.13-248.65
spellingShingle	glycogen synthase kinase-3β inhibitor (GSKi) polydopamine 316L stainless steel endothelialization human coronary artery endothelial cells Biotechnology TP248.13-248.65 Ming Zhang Xudong Shi Xudong Shi Hai Sun Hai Sun Donghua Xu Yang Gao Xi Wu Jianqi Zhang Jichang Zhang Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
description	The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accelerate vascular endothelialization. In this work, GSKi was covalently conjugated on 316L stainless steel through polydopamine to develop a stable bioactive surface. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact angle results revealed the successful introduction of GSKi onto 316L stainless steel. The GSKi coating did not obviously affect the hemocompatibility of plates. The adhesion and proliferation of human coronary artery endothelial cells (HCAECs) on stainless steel was significantly promoted by the addition of GSKi. In summary, this work provides a universal and stable strategy of immobilizing GSKi on the stent surface. This method has the potential for widespread application in the modification of vascular stents.
format	article
author	Ming Zhang Xudong Shi Xudong Shi Hai Sun Hai Sun Donghua Xu Yang Gao Xi Wu Jianqi Zhang Jichang Zhang
author_facet	Ming Zhang Xudong Shi Xudong Shi Hai Sun Hai Sun Donghua Xu Yang Gao Xi Wu Jianqi Zhang Jichang Zhang
author_sort	Ming Zhang
title	Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_short	Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_full	Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_fullStr	Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_full_unstemmed	Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization
title_sort	immobilization of glycogen synthase kinase-3β inhibitor on 316l stainless steel via polydopamine to accelerate endothelialization
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5f242408e0114735afa8216c6d99819b
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Immobilization of Glycogen Synthase Kinase-3β Inhibitor on 316L Stainless Steel via Polydopamine to Accelerate Endothelialization

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