Color-tuning of natural variants of heliorhodopsin

Abstract Microbial rhodopsins are distributed through many microorganisms. Heliorhodopsins are newly discovered but have an unclear function. They have seven transmembrane helices similar to type-I and type-II rhodopsins, but they are different in that the N-terminal region of heliorhodopsin is cyto...

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Autores principales:	Se-Hwan Kim, Kimleng Chuon, Shin-Gyu Cho, Ahreum Choi, Seanghun Meas, Hyun-Suk Cho, Kwang-Hwan Jung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f25e88d7a17476ebd70c9f8ee73108c
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spelling	oai:doaj.org-article:5f25e88d7a17476ebd70c9f8ee73108c2021-12-02T14:01:33ZColor-tuning of natural variants of heliorhodopsin10.1038/s41598-020-72125-02045-2322https://doaj.org/article/5f25e88d7a17476ebd70c9f8ee73108c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72125-0https://doaj.org/toc/2045-2322Abstract Microbial rhodopsins are distributed through many microorganisms. Heliorhodopsins are newly discovered but have an unclear function. They have seven transmembrane helices similar to type-I and type-II rhodopsins, but they are different in that the N-terminal region of heliorhodopsin is cytoplasmic. We chose 13 representative heliorhodopsins from various microorganisms, expressed and purified with an N-terminal His tag, and measured the absorption spectra. The 13 natural variants had an absorption maximum (λmax) in the range 530–556 nm similar to proteorhodopsin (λmax = 490–525 nm). We selected several candidate residues that influence rhodopsin color-tuning based on sequence alignment and constructed mutants via site-directed mutagenesis to confirm the spectral changes. We found two important residues located near retinal chromophore that influence λmax. We also predict the 3D structure via homology-modeling of Thermoplasmatales heliorhodopsin. The results indicate that the color-tuning mechanism of type-I rhodopsin can be applied to understand the color-tuning of heliorhodopsin.Se-Hwan KimKimleng ChuonShin-Gyu ChoAhreum ChoiSeanghun MeasHyun-Suk ChoKwang-Hwan JungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Se-Hwan Kim Kimleng Chuon Shin-Gyu Cho Ahreum Choi Seanghun Meas Hyun-Suk Cho Kwang-Hwan Jung Color-tuning of natural variants of heliorhodopsin
description	Abstract Microbial rhodopsins are distributed through many microorganisms. Heliorhodopsins are newly discovered but have an unclear function. They have seven transmembrane helices similar to type-I and type-II rhodopsins, but they are different in that the N-terminal region of heliorhodopsin is cytoplasmic. We chose 13 representative heliorhodopsins from various microorganisms, expressed and purified with an N-terminal His tag, and measured the absorption spectra. The 13 natural variants had an absorption maximum (λmax) in the range 530–556 nm similar to proteorhodopsin (λmax = 490–525 nm). We selected several candidate residues that influence rhodopsin color-tuning based on sequence alignment and constructed mutants via site-directed mutagenesis to confirm the spectral changes. We found two important residues located near retinal chromophore that influence λmax. We also predict the 3D structure via homology-modeling of Thermoplasmatales heliorhodopsin. The results indicate that the color-tuning mechanism of type-I rhodopsin can be applied to understand the color-tuning of heliorhodopsin.
format	article
author	Se-Hwan Kim Kimleng Chuon Shin-Gyu Cho Ahreum Choi Seanghun Meas Hyun-Suk Cho Kwang-Hwan Jung
author_facet	Se-Hwan Kim Kimleng Chuon Shin-Gyu Cho Ahreum Choi Seanghun Meas Hyun-Suk Cho Kwang-Hwan Jung
author_sort	Se-Hwan Kim
title	Color-tuning of natural variants of heliorhodopsin
title_short	Color-tuning of natural variants of heliorhodopsin
title_full	Color-tuning of natural variants of heliorhodopsin
title_fullStr	Color-tuning of natural variants of heliorhodopsin
title_full_unstemmed	Color-tuning of natural variants of heliorhodopsin
title_sort	color-tuning of natural variants of heliorhodopsin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5f25e88d7a17476ebd70c9f8ee73108c
work_keys_str_mv	AT sehwankim colortuningofnaturalvariantsofheliorhodopsin AT kimlengchuon colortuningofnaturalvariantsofheliorhodopsin AT shingyucho colortuningofnaturalvariantsofheliorhodopsin AT ahreumchoi colortuningofnaturalvariantsofheliorhodopsin AT seanghunmeas colortuningofnaturalvariantsofheliorhodopsin AT hyunsukcho colortuningofnaturalvariantsofheliorhodopsin AT kwanghwanjung colortuningofnaturalvariantsofheliorhodopsin
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Color-tuning of natural variants of heliorhodopsin

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