EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD

Objective: To develop and evaluate a new high-performance liquid chromatography (HPLC) based method for Voriconazole (VRC) analysis in blood. Study Design: Cross sectional study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology Armed Forces Institute of Pathology...

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Autores principales:	Ambreen Rehman, Naveed Asif, Ayesha Hafeez, Aamir Ijaz, Muhammad Waseem, Qurat Ul Ain
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	high performance liquid chromatography therapeutic drug monitoring Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5f26cfa7924e47cd92293b3c22ba5cd4
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spelling	oai:doaj.org-article:5f26cfa7924e47cd92293b3c22ba5cd42021-11-12T02:51:36ZEVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD0030-96482411-8842https://doaj.org/article/5f26cfa7924e47cd92293b3c22ba5cd42019-06-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3019/2257https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To develop and evaluate a new high-performance liquid chromatography (HPLC) based method for Voriconazole (VRC) analysis in blood. Study Design: Cross sectional study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology Armed Forces Institute of Pathology (AFIP), Rawalpindi, from May 2017 to Oct 2017. Material and Methods: Trough venous blood samples of twenty-five patients on VRC therapy were collected, after five days of starting VRC. Samples were centrifuged. About 100 μl of Lorazepam as internal standard (5.0 μg/ml), was added to 0.5ml of each sample. Then extraction was done with 4.0 ml of mixture of hexane and ethyl acetate. Centrifugation was again done and supernatant layer was collected and dried under nitrogen. It was reconstituted with 200μl of deionized water and acetonitrile. Samples were analyzed on HPLC. Results: Method was validated in terms of linearity, limit of detection, limit of quantification, precision, accuracy, recovery, analytical specificity and stability. Linearity calibration curve plot for assay obtained was linear over analytical measurement range of 1.0 to 8.0μg/ml and correlation coefficient was 0.99. Mean intra-assay accuracy for 1.0, 5.0 and 8.0 μg/ml was 96%, 98.6%, 99.5% respectively, and percentage Relative Standard Deviation (RSD) was 5.9%, 1.15%, 1.44% respectively. Mean inter-assay accuracy for 1.0, 5.0 and 8.0 μg/ml was 95%, 98.6%, 99.5% respectively, and % RSD was 5.68%, 1.03% and 1.01% respectively. Limit of Detection (LOD) was 0.25 μg/mL and Limit of Quantitation (LOQ) was 1.0 μg/ml. Conclusion: HPLC based method for VRC determination is accurate and specific with good clinical correlation. This method may be used in routine for Therapeutic Drug Monitoring (TDM) of VRC.Ambreen RehmanNaveed AsifAyesha HafeezAamir IjazMuhammad WaseemQurat Ul AinArmy Medical College Rawalpindiarticlehigh performance liquid chromatographytherapeutic drug monitoringMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 3, Pp 516-521 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	high performance liquid chromatography therapeutic drug monitoring Medicine R Medicine (General) R5-920
spellingShingle	high performance liquid chromatography therapeutic drug monitoring Medicine R Medicine (General) R5-920 Ambreen Rehman Naveed Asif Ayesha Hafeez Aamir Ijaz Muhammad Waseem Qurat Ul Ain EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD
description	Objective: To develop and evaluate a new high-performance liquid chromatography (HPLC) based method for Voriconazole (VRC) analysis in blood. Study Design: Cross sectional study. Place and Duration of Study: Department of Chemical Pathology and Endocrinology Armed Forces Institute of Pathology (AFIP), Rawalpindi, from May 2017 to Oct 2017. Material and Methods: Trough venous blood samples of twenty-five patients on VRC therapy were collected, after five days of starting VRC. Samples were centrifuged. About 100 μl of Lorazepam as internal standard (5.0 μg/ml), was added to 0.5ml of each sample. Then extraction was done with 4.0 ml of mixture of hexane and ethyl acetate. Centrifugation was again done and supernatant layer was collected and dried under nitrogen. It was reconstituted with 200μl of deionized water and acetonitrile. Samples were analyzed on HPLC. Results: Method was validated in terms of linearity, limit of detection, limit of quantification, precision, accuracy, recovery, analytical specificity and stability. Linearity calibration curve plot for assay obtained was linear over analytical measurement range of 1.0 to 8.0μg/ml and correlation coefficient was 0.99. Mean intra-assay accuracy for 1.0, 5.0 and 8.0 μg/ml was 96%, 98.6%, 99.5% respectively, and percentage Relative Standard Deviation (RSD) was 5.9%, 1.15%, 1.44% respectively. Mean inter-assay accuracy for 1.0, 5.0 and 8.0 μg/ml was 95%, 98.6%, 99.5% respectively, and % RSD was 5.68%, 1.03% and 1.01% respectively. Limit of Detection (LOD) was 0.25 μg/mL and Limit of Quantitation (LOQ) was 1.0 μg/ml. Conclusion: HPLC based method for VRC determination is accurate and specific with good clinical correlation. This method may be used in routine for Therapeutic Drug Monitoring (TDM) of VRC.
format	article
author	Ambreen Rehman Naveed Asif Ayesha Hafeez Aamir Ijaz Muhammad Waseem Qurat Ul Ain
author_facet	Ambreen Rehman Naveed Asif Ayesha Hafeez Aamir Ijaz Muhammad Waseem Qurat Ul Ain
author_sort	Ambreen Rehman
title	EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD
title_short	EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD
title_full	EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD
title_fullStr	EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD
title_full_unstemmed	EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD
title_sort	evaluation of a new high performance liquid chromatography based method for voriconazole analysis in blood
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/5f26cfa7924e47cd92293b3c22ba5cd4
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_version_	1718431292792504320

EVALUATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHY BASED METHOD FOR VORICONAZOLE ANALYSIS IN BLOOD

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