EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles

Wen-Hsuan Tsai,1 Kun-Hua Yu,1 Yi-Cheng Huang,2 Cheng-I Lee1 1Department of Biomedical Sciences, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan, Republic of China; 2Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan, Republic of China Background: Photod...

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Autores principales:	Tsai WH, Yu KH, Huang YC, Lee CI
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	EGFR-targeting cancer photodynamic therapy curcumin chitosan Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5f2e340657ad4478ab4f1ab86becd16d
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spelling	oai:doaj.org-article:5f2e340657ad4478ab4f1ab86becd16d2021-12-02T05:09:47ZEGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles1178-2013https://doaj.org/article/5f2e340657ad4478ab4f1ab86becd16d2018-02-01T00:00:00Zhttps://www.dovepress.com/egfr-targeted-photodynamic-therapy-by-curcumin-encapsulated-chitosantp-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wen-Hsuan Tsai,1 Kun-Hua Yu,1 Yi-Cheng Huang,2 Cheng-I Lee1 1Department of Biomedical Sciences, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan, Republic of China; 2Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan, Republic of China Background: Photodynamic therapy (PDT) is an effective therapy for cancers and is a minimally invasive therapy with low dark toxicity and limited side effects. PDT employs the combination of photosensitizers with a specific light source to produce reactive oxygen species (ROS) to damage tumor cells. Methods: We fabricated nanoparticles encapsulating curcumin through crosslinking chitosan and tripolyphosphate (TPP). Additionally, the chitosan was conjugated to epidermal growth factor in order to target the epidermal growth factor receptor (EGFR), overexpressed on cancer cells. To investigate PDT using fabricated nanoparticles, we measured cell viabilities and ROS production in relation to EGFR-overexpressing gastric cancer cells and non-cancer gastric cells.Results: The targeting nanoparticles displayed a superior PDT effect in the cancer cell, with a resultant approximately fourfold decrease in the IC50. The PDT mechanism of curcumin-encapsulated nanoparticles is further identified as the generation of 1O2, the major pathway in PDT.Conclusion: These curcumin-encapsulated chitosan/TPP nanoparticles are a promising targeted-PDT against EGFR-overexpressing cancers. Keywords: EGFR-targeting, cancer, photodynamic therapy, curcumin, chitosan Tsai WHYu KHHuang YCLee CIDove Medical PressarticleEGFR-targetingcancerphotodynamic therapycurcuminchitosanMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 903-916 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	EGFR-targeting cancer photodynamic therapy curcumin chitosan Medicine (General) R5-920
spellingShingle	EGFR-targeting cancer photodynamic therapy curcumin chitosan Medicine (General) R5-920 Tsai WH Yu KH Huang YC Lee CI EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles
description	Wen-Hsuan Tsai,1 Kun-Hua Yu,1 Yi-Cheng Huang,2 Cheng-I Lee1 1Department of Biomedical Sciences, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan, Republic of China; 2Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan, Republic of China Background: Photodynamic therapy (PDT) is an effective therapy for cancers and is a minimally invasive therapy with low dark toxicity and limited side effects. PDT employs the combination of photosensitizers with a specific light source to produce reactive oxygen species (ROS) to damage tumor cells. Methods: We fabricated nanoparticles encapsulating curcumin through crosslinking chitosan and tripolyphosphate (TPP). Additionally, the chitosan was conjugated to epidermal growth factor in order to target the epidermal growth factor receptor (EGFR), overexpressed on cancer cells. To investigate PDT using fabricated nanoparticles, we measured cell viabilities and ROS production in relation to EGFR-overexpressing gastric cancer cells and non-cancer gastric cells.Results: The targeting nanoparticles displayed a superior PDT effect in the cancer cell, with a resultant approximately fourfold decrease in the IC50. The PDT mechanism of curcumin-encapsulated nanoparticles is further identified as the generation of 1O2, the major pathway in PDT.Conclusion: These curcumin-encapsulated chitosan/TPP nanoparticles are a promising targeted-PDT against EGFR-overexpressing cancers. Keywords: EGFR-targeting, cancer, photodynamic therapy, curcumin, chitosan
format	article
author	Tsai WH Yu KH Huang YC Lee CI
author_facet	Tsai WH Yu KH Huang YC Lee CI
author_sort	Tsai WH
title	EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles
title_short	EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles
title_full	EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles
title_fullStr	EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles
title_full_unstemmed	EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles
title_sort	egfr-targeted photodynamic therapy by curcumin-encapsulated chitosan/tpp nanoparticles
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/5f2e340657ad4478ab4f1ab86becd16d
work_keys_str_mv	AT tsaiwh egfrtargetedphotodynamictherapybycurcuminencapsulatedchitosantppnanoparticles AT yukh egfrtargetedphotodynamictherapybycurcuminencapsulatedchitosantppnanoparticles AT huangyc egfrtargetedphotodynamictherapybycurcuminencapsulatedchitosantppnanoparticles AT leeci egfrtargetedphotodynamictherapybycurcuminencapsulatedchitosantppnanoparticles
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EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles

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