Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.

<h4>Background</h4>The objective of this study was to establish the buffalo mammary epithelial cell line (BuMEC) and characterize its mammary specific functions.<h4>Methodology</h4>Buffalo mammary tissue collected from the slaughter house was processed enzymatically to obtain...

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Autores principales:	Vijay Anand, Nilambra Dogra, Surender Singh, Sudarshan N Kumar, Manoj K Jena, Dhruba Malakar, Ajay K Dang, Bishnu P Mishra, Tapas K Mukhopadhyay, Jai K Kaushik, Ashok K Mohanty
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f2e8f01e06e4006bdb371ef6b78b190
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spelling	oai:doaj.org-article:5f2e8f01e06e4006bdb371ef6b78b1902021-11-18T07:13:05ZEstablishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.1932-620310.1371/journal.pone.0040469https://doaj.org/article/5f2e8f01e06e4006bdb371ef6b78b1902012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22792341/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The objective of this study was to establish the buffalo mammary epithelial cell line (BuMEC) and characterize its mammary specific functions.<h4>Methodology</h4>Buffalo mammary tissue collected from the slaughter house was processed enzymatically to obtain a heterogenous population of cells containing both epithelial and fibroblasts cells. Epithelial cells were purified by selective trypsinization and were grown in a plastic substratum. The purified mammary epithelial cells (MECs) after several passages were characterized for mammary specific functions by immunocytochemistry, RT-PCR and western blot.<h4>Principal findings</h4>The established buffalo mammary epithelial cell line (BuMEC) exhibited epithelial cell characteristics by immunostaining positively with cytokeratin 18 and negatively with vimentin. The BuMEC maintained the characteristics of its functional differentiation by expression of β-casein, κ-casein, butyrophilin and lactoferrin. BuMEC had normal growth properties and maintained diploid chromosome number (2n = 50) before and after cryopreservation. A spontaneously immortalized buffalo mammary epithelial cell line was established after 20 passages and was continuously subcultured for more than 60 passages without senescence.<h4>Conclusions</h4>We have established a buffalo mammary epithelial cell line that can be used as a model system for studying mammary gland functions.Vijay AnandNilambra DograSurender SinghSudarshan N KumarManoj K JenaDhruba MalakarAjay K DangBishnu P MishraTapas K MukhopadhyayJai K KaushikAshok K MohantyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40469 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vijay Anand Nilambra Dogra Surender Singh Sudarshan N Kumar Manoj K Jena Dhruba Malakar Ajay K Dang Bishnu P Mishra Tapas K Mukhopadhyay Jai K Kaushik Ashok K Mohanty Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
description	<h4>Background</h4>The objective of this study was to establish the buffalo mammary epithelial cell line (BuMEC) and characterize its mammary specific functions.<h4>Methodology</h4>Buffalo mammary tissue collected from the slaughter house was processed enzymatically to obtain a heterogenous population of cells containing both epithelial and fibroblasts cells. Epithelial cells were purified by selective trypsinization and were grown in a plastic substratum. The purified mammary epithelial cells (MECs) after several passages were characterized for mammary specific functions by immunocytochemistry, RT-PCR and western blot.<h4>Principal findings</h4>The established buffalo mammary epithelial cell line (BuMEC) exhibited epithelial cell characteristics by immunostaining positively with cytokeratin 18 and negatively with vimentin. The BuMEC maintained the characteristics of its functional differentiation by expression of β-casein, κ-casein, butyrophilin and lactoferrin. BuMEC had normal growth properties and maintained diploid chromosome number (2n = 50) before and after cryopreservation. A spontaneously immortalized buffalo mammary epithelial cell line was established after 20 passages and was continuously subcultured for more than 60 passages without senescence.<h4>Conclusions</h4>We have established a buffalo mammary epithelial cell line that can be used as a model system for studying mammary gland functions.
format	article
author	Vijay Anand Nilambra Dogra Surender Singh Sudarshan N Kumar Manoj K Jena Dhruba Malakar Ajay K Dang Bishnu P Mishra Tapas K Mukhopadhyay Jai K Kaushik Ashok K Mohanty
author_facet	Vijay Anand Nilambra Dogra Surender Singh Sudarshan N Kumar Manoj K Jena Dhruba Malakar Ajay K Dang Bishnu P Mishra Tapas K Mukhopadhyay Jai K Kaushik Ashok K Mohanty
author_sort	Vijay Anand
title	Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
title_short	Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
title_full	Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
title_fullStr	Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
title_full_unstemmed	Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.
title_sort	establishment and characterization of a buffalo (bubalus bubalis) mammary epithelial cell line.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5f2e8f01e06e4006bdb371ef6b78b190
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Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line.

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