Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents

Danielle AMT Ragioto, Letícia DM Carrasco, Ana M Carmona-Ribeiro Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil Abstract: Dioctadecyldimethylammonium bromide (DODAB) is an antimicrobial lipid t...

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Autores principales:	Ragioto DA, Carrasco LD, Carmona-Ribeiro AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5f3320f5e0f44d7b89ee19d5dff5deb2
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spelling	oai:doaj.org-article:5f3320f5e0f44d7b89ee19d5dff5deb22021-12-02T01:32:10ZNovel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents1178-2013https://doaj.org/article/5f3320f5e0f44d7b89ee19d5dff5deb22014-06-01T00:00:00Zhttp://www.dovepress.com/novel-gramicidin-formulations-in-cationic-lipid-as-broad-spectrum-micr-a17417https://doaj.org/toc/1178-2013 Danielle AMT Ragioto, Letícia DM Carrasco, Ana M Carmona-Ribeiro Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil Abstract: Dioctadecyldimethylammonium bromide (DODAB) is an antimicrobial lipid that can be dispersed as large closed bilayers (LV) or bilayer disks (BF). Gramicidin (Gr) is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. In mammalian cells, DODAB and Gr have the drawbacks of Gram-positive resistance and high toxicity, respectively. In this study, DODAB bilayers incorporating Gr showed good antimicrobial activity and low toxicity. Techniques employed were spectroscopy, photon correlation spectroscopy for sizing and evaluation of the surface potential at the shear plane, turbidimetric detection of dissipation of osmotic gradients in LV/Gr, determination of bacterial cell lysis, and counting of colony-forming units. There was quantitative incorporation of Gr and development of functional channels in LV. Gr increased the bilayer charge density in LV but did not affect the BF charge density, with localization of Gr at the BF borders. DODAB/Gr formulations substantially reduce Gr toxicity against eukaryotic cells and advantageously broaden the antimicrobial activity spectrum, effectively killing Escherichia coli and Staphylococcus aureus bacteria with occurrence of cell lysis. Keywords: antimicrobial peptides, cationic bilayers, bilayer fragments, dioctadecyldimethylammonium bromide, gramicidin A, self-assemblyRagioto DACarrasco LDCarmona-Ribeiro AMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3183-3192 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ragioto DA Carrasco LD Carmona-Ribeiro AM Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
description	Danielle AMT Ragioto, Letícia DM Carrasco, Ana M Carmona-Ribeiro Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil Abstract: Dioctadecyldimethylammonium bromide (DODAB) is an antimicrobial lipid that can be dispersed as large closed bilayers (LV) or bilayer disks (BF). Gramicidin (Gr) is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. In mammalian cells, DODAB and Gr have the drawbacks of Gram-positive resistance and high toxicity, respectively. In this study, DODAB bilayers incorporating Gr showed good antimicrobial activity and low toxicity. Techniques employed were spectroscopy, photon correlation spectroscopy for sizing and evaluation of the surface potential at the shear plane, turbidimetric detection of dissipation of osmotic gradients in LV/Gr, determination of bacterial cell lysis, and counting of colony-forming units. There was quantitative incorporation of Gr and development of functional channels in LV. Gr increased the bilayer charge density in LV but did not affect the BF charge density, with localization of Gr at the BF borders. DODAB/Gr formulations substantially reduce Gr toxicity against eukaryotic cells and advantageously broaden the antimicrobial activity spectrum, effectively killing Escherichia coli and Staphylococcus aureus bacteria with occurrence of cell lysis. Keywords: antimicrobial peptides, cationic bilayers, bilayer fragments, dioctadecyldimethylammonium bromide, gramicidin A, self-assembly
format	article
author	Ragioto DA Carrasco LD Carmona-Ribeiro AM
author_facet	Ragioto DA Carrasco LD Carmona-Ribeiro AM
author_sort	Ragioto DA
title	Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_short	Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_full	Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_fullStr	Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_full_unstemmed	Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
title_sort	novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/5f3320f5e0f44d7b89ee19d5dff5deb2
work_keys_str_mv	AT ragiotoda novelgramicidinformulationsincationiclipidasbroadspectrummicrobicidalagents AT carrascold novelgramicidinformulationsincationiclipidasbroadspectrummicrobicidalagents AT carmonaribeiroam novelgramicidinformulationsincationiclipidasbroadspectrummicrobicidalagents
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Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents

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