Association of Circulating MicroRNA-142-3p with Graves Disease

This study aims to investigate the possible role of circulating microRNA-142-3p (miR-142-3p) in the development of graves disease (GD) and its association with the antibody directed against thyroid stimulating hormone receptor (TSHR-Ab) production in patients with GD. Forty patients with positive T...

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Autores principales:	Rashad Ayad Al-Heety, Hayfaa S Al-Hadithi
Formato:	article
Lenguaje:	AR EN
Publicado:	College of Science for Women, University of Baghdad 2021
Materias:	Circulating microRNAs, MicroRNA-142-3p, Graves disease, Thyroid stimulating hormone receptor antibody, Reverse transcription polymerase chain reaction Science Q
Acceso en línea:	https://doaj.org/article/5f3a8fca45cf4f4e88f1afac3c44a2ad
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spelling	oai:doaj.org-article:5f3a8fca45cf4f4e88f1afac3c44a2ad2021-12-04T16:13:00ZAssociation of Circulating MicroRNA-142-3p with Graves Disease10.21123/bsj.2021.18.4.11332078-86652411-7986https://doaj.org/article/5f3a8fca45cf4f4e88f1afac3c44a2ad2021-12-01T00:00:00Zhttps://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4580https://doaj.org/toc/2078-8665https://doaj.org/toc/2411-7986 This study aims to investigate the possible role of circulating microRNA-142-3p (miR-142-3p) in the development of graves disease (GD) and its association with the antibody directed against thyroid stimulating hormone receptor (TSHR-Ab) production in patients with GD. Forty patients with positive TSHR-Ab enrolled in this study were divided ,based on treatment, into (22 untreated (newly diagnosed) and 18 treated patients) and based on family history (30 with positive family history and 10 with negative family history). In addition to forty healthy subjects with sex and age matching as a control group. The expression level of circulating miR-142-3p was determined by two steps reverse transcription polymerase chain reaction (RT-PCR) technique. Results show that there is a significant elevation (p < 0.01) in the expression of miR- 142-3p in the serum of both treated and untreated patients compared with controls and in patients with positive family history compared with negative family history. While its expression is non-significantly lower (P > 0.05) in the serum of treated patients compared with untreated ones. It has been found that miR- 142-3p expression was positively correlated with levels of TSHR-Ab, FT3, and FT4. In addition, the miR- 142-3p expression has a good diagnostic accuracy with sensitivity (82%) and specificity (80%). In conclusion, the differential expression of miR-142-3p between patients and healthy controls appears as a potential biomarker for diagnosis of GD and the positive correlation of miR-142-3p with TSHR-Ab suggesting the contribution of this miRNA in the development of GD. Rashad Ayad Al-HeetyHayfaa S Al-HadithiCollege of Science for Women, University of BaghdadarticleCirculating microRNAs, MicroRNA-142-3p, Graves disease, Thyroid stimulating hormone receptor antibody, Reverse transcription polymerase chain reactionScienceQARENBaghdad Science Journal, Vol 18, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	AR EN
topic	Circulating microRNAs, MicroRNA-142-3p, Graves disease, Thyroid stimulating hormone receptor antibody, Reverse transcription polymerase chain reaction Science Q
spellingShingle	Circulating microRNAs, MicroRNA-142-3p, Graves disease, Thyroid stimulating hormone receptor antibody, Reverse transcription polymerase chain reaction Science Q Rashad Ayad Al-Heety Hayfaa S Al-Hadithi Association of Circulating MicroRNA-142-3p with Graves Disease
description	This study aims to investigate the possible role of circulating microRNA-142-3p (miR-142-3p) in the development of graves disease (GD) and its association with the antibody directed against thyroid stimulating hormone receptor (TSHR-Ab) production in patients with GD. Forty patients with positive TSHR-Ab enrolled in this study were divided ,based on treatment, into (22 untreated (newly diagnosed) and 18 treated patients) and based on family history (30 with positive family history and 10 with negative family history). In addition to forty healthy subjects with sex and age matching as a control group. The expression level of circulating miR-142-3p was determined by two steps reverse transcription polymerase chain reaction (RT-PCR) technique. Results show that there is a significant elevation (p < 0.01) in the expression of miR- 142-3p in the serum of both treated and untreated patients compared with controls and in patients with positive family history compared with negative family history. While its expression is non-significantly lower (P > 0.05) in the serum of treated patients compared with untreated ones. It has been found that miR- 142-3p expression was positively correlated with levels of TSHR-Ab, FT3, and FT4. In addition, the miR- 142-3p expression has a good diagnostic accuracy with sensitivity (82%) and specificity (80%). In conclusion, the differential expression of miR-142-3p between patients and healthy controls appears as a potential biomarker for diagnosis of GD and the positive correlation of miR-142-3p with TSHR-Ab suggesting the contribution of this miRNA in the development of GD.
format	article
author	Rashad Ayad Al-Heety Hayfaa S Al-Hadithi
author_facet	Rashad Ayad Al-Heety Hayfaa S Al-Hadithi
author_sort	Rashad Ayad Al-Heety
title	Association of Circulating MicroRNA-142-3p with Graves Disease
title_short	Association of Circulating MicroRNA-142-3p with Graves Disease
title_full	Association of Circulating MicroRNA-142-3p with Graves Disease
title_fullStr	Association of Circulating MicroRNA-142-3p with Graves Disease
title_full_unstemmed	Association of Circulating MicroRNA-142-3p with Graves Disease
title_sort	association of circulating microrna-142-3p with graves disease
publisher	College of Science for Women, University of Baghdad
publishDate	2021
url	https://doaj.org/article/5f3a8fca45cf4f4e88f1afac3c44a2ad
work_keys_str_mv	AT rashadayadalheety associationofcirculatingmicrorna1423pwithgravesdisease AT hayfaasalhadithi associationofcirculatingmicrorna1423pwithgravesdisease
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Association of Circulating MicroRNA-142-3p with Graves Disease

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