Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).

Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM) and to eva...

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Autores principales:	Germana Freire Rocha Caldas, Alisson Rodrigo da Silva Oliveira, Alice Valença Araújo, Dafne Carolina Alves Quixabeira, Jacinto da Costa Silva-Neto, João Henrique Costa-Silva, Irwin Rose Alencar de Menezes, Fabiano Ferreira, Ana Cristina Lima Leite, José Galberto Martins da Costa, Almir Gonçalves Wanderley
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f4045b611b743f387d819941b1bbc4a
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spelling	oai:doaj.org-article:5f4045b611b743f387d819941b1bbc4a2021-11-18T08:37:46ZGastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).1932-620310.1371/journal.pone.0084400https://doaj.org/article/5f4045b611b743f387d819941b1bbc4a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454726/?tool=EBIhttps://doaj.org/toc/1932-6203Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM) and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control), pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model) or EOHM. The antisecretory activity (basal or stimulated) was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (-SH groups), as well as the quantification of adherent mucus and the levels of malondialdehyde and -SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA). EOHM (400 mg/kg) reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%), reduced levels of MDA (72.5%) and prevented the depletion of -SH groups (73.8%) in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.Germana Freire Rocha CaldasAlisson Rodrigo da Silva OliveiraAlice Valença AraújoDafne Carolina Alves QuixabeiraJacinto da Costa Silva-NetoJoão Henrique Costa-SilvaIrwin Rose Alencar de MenezesFabiano FerreiraAna Cristina Lima LeiteJosé Galberto Martins da CostaAlmir Gonçalves WanderleyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84400 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Germana Freire Rocha Caldas Alisson Rodrigo da Silva Oliveira Alice Valença Araújo Dafne Carolina Alves Quixabeira Jacinto da Costa Silva-Neto João Henrique Costa-Silva Irwin Rose Alencar de Menezes Fabiano Ferreira Ana Cristina Lima Leite José Galberto Martins da Costa Almir Gonçalves Wanderley Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).
description	Hyptis martiusii Benth. is an aromatic plant found in abundance in northeastern Brazil that is used in ethnomedicine to treat gastric disorders. The aim of this study was to elucidate the mechanisms of action involved in the gastroprotection of the essential oil of Hyptis martiusii (EOHM) and to evaluate its healing capacity. Wistar rats were exposed to different protocols and subsequently were treated with 1% Tween-80 aqueous solution (negative control), pantoprazole, carbenoxolone, N-acetylcysteine (depending on the specificity of each model) or EOHM. The antisecretory activity (basal or stimulated) was determined using the pyloric ligature method. The gastroprotective action of nitric oxide and sulphydryl groups (-SH groups), as well as the quantification of adherent mucus and the levels of malondialdehyde and -SH groups in gastric mucosa, were evaluated using ethanol-induced gastric ulcer model. The healing ability was evaluated using the acetic acid-induced gastric ulcer model and histological and immunohistochemical analysis (HE, PAS and PCNA). EOHM (400 mg/kg) reduced the volume and acidity of gastric secretion stimulated by histamine and pentagastrin. The gastroprotective effect of EOHM involves the participation of endogenous sulfhydryl groups. EOHM increased mucus production (54.8%), reduced levels of MDA (72.5%) and prevented the depletion of -SH groups (73.8%) in the gastric mucosa. The treatment with EOHM reduced in 70.3% the gastric lesion area, promoting significant regeneration of the gastric mucosa, as confirmed by histological analysis and analysis of proliferating cell nuclear antigen. The results show that gastroprotective effect of EOHM is mediated by cytoprotective and antioxidant mechanisms and by their antisecretory activity, and suggest that the essential oil of Hyptis martiusii is a promising candidate for the treatment of gastric ulcers.
format	article
author	Germana Freire Rocha Caldas Alisson Rodrigo da Silva Oliveira Alice Valença Araújo Dafne Carolina Alves Quixabeira Jacinto da Costa Silva-Neto João Henrique Costa-Silva Irwin Rose Alencar de Menezes Fabiano Ferreira Ana Cristina Lima Leite José Galberto Martins da Costa Almir Gonçalves Wanderley
author_facet	Germana Freire Rocha Caldas Alisson Rodrigo da Silva Oliveira Alice Valença Araújo Dafne Carolina Alves Quixabeira Jacinto da Costa Silva-Neto João Henrique Costa-Silva Irwin Rose Alencar de Menezes Fabiano Ferreira Ana Cristina Lima Leite José Galberto Martins da Costa Almir Gonçalves Wanderley
author_sort	Germana Freire Rocha Caldas
title	Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).
title_short	Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).
title_full	Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).
title_fullStr	Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).
title_full_unstemmed	Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).
title_sort	gastroprotective and ulcer healing effects of essential oil of hyptis martiusii benth. (lamiaceae).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5f4045b611b743f387d819941b1bbc4a
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Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae).

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