Current Status of Breast Organoid Models

Breast cancer (BC) is the most frequently diagnosed malignancy among women globally. Although mouse models have been critical in advancing the knowledge of BC tumorigenesis and progression, human breast models comprising the breast tissue microenvironment are needed to help elucidate the underlying...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Srivarshini Cherukupalli Mohan, Tian-Yu Lee, Armando E. Giuliano, Xiaojiang Cui
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	breast organoids 3D culture cancer matrix mammary organoids Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5f47b17769754222a1e82e54d208dedb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5f47b17769754222a1e82e54d208dedb
record_format	dspace
spelling	oai:doaj.org-article:5f47b17769754222a1e82e54d208dedb2021-11-05T06:32:48ZCurrent Status of Breast Organoid Models2296-418510.3389/fbioe.2021.745943https://doaj.org/article/5f47b17769754222a1e82e54d208dedb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.745943/fullhttps://doaj.org/toc/2296-4185Breast cancer (BC) is the most frequently diagnosed malignancy among women globally. Although mouse models have been critical in advancing the knowledge of BC tumorigenesis and progression, human breast models comprising the breast tissue microenvironment are needed to help elucidate the underlying mechanisms of BC risk factors. As such, it is essential to identify an ex vivo human breast tissue mimetic model that can accurately pinpoint the effects of these factors in BC development. While two-dimensional models have been invaluable, they are not suitable for studying patient-specific tumor biology and drug response. Recent developments in three-dimensional (3D) models have led to the prominence of organized structures grown in a 3D environment called “organoids.” Breast organoids can accurately recapitulate the in vivo breast microenvironment and have been used to examine factors that affect signaling transduction, gene expression, and tissue remodeling. In this review, the applications, components, and protocols for development of breast organoids are discussed. We summarize studies that describe the utility of breast organoids, including in the study of normal mammary gland development and tumorigenesis. Finally, we provide an overview of protocols for development of breast organoids, and the advantages and disadvantages of different techniques in studies are described. The included studies have shown that breast organoids will continue to serve as a crucial platform for understanding of progression of BC tumors and the testing of novel therapeutics.Srivarshini Cherukupalli MohanTian-Yu LeeArmando E. GiulianoXiaojiang CuiFrontiers Media S.A.articlebreast organoids3D culturecancermatrixmammary organoidsBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast organoids 3D culture cancer matrix mammary organoids Biotechnology TP248.13-248.65
spellingShingle	breast organoids 3D culture cancer matrix mammary organoids Biotechnology TP248.13-248.65 Srivarshini Cherukupalli Mohan Tian-Yu Lee Armando E. Giuliano Xiaojiang Cui Current Status of Breast Organoid Models
description	Breast cancer (BC) is the most frequently diagnosed malignancy among women globally. Although mouse models have been critical in advancing the knowledge of BC tumorigenesis and progression, human breast models comprising the breast tissue microenvironment are needed to help elucidate the underlying mechanisms of BC risk factors. As such, it is essential to identify an ex vivo human breast tissue mimetic model that can accurately pinpoint the effects of these factors in BC development. While two-dimensional models have been invaluable, they are not suitable for studying patient-specific tumor biology and drug response. Recent developments in three-dimensional (3D) models have led to the prominence of organized structures grown in a 3D environment called “organoids.” Breast organoids can accurately recapitulate the in vivo breast microenvironment and have been used to examine factors that affect signaling transduction, gene expression, and tissue remodeling. In this review, the applications, components, and protocols for development of breast organoids are discussed. We summarize studies that describe the utility of breast organoids, including in the study of normal mammary gland development and tumorigenesis. Finally, we provide an overview of protocols for development of breast organoids, and the advantages and disadvantages of different techniques in studies are described. The included studies have shown that breast organoids will continue to serve as a crucial platform for understanding of progression of BC tumors and the testing of novel therapeutics.
format	article
author	Srivarshini Cherukupalli Mohan Tian-Yu Lee Armando E. Giuliano Xiaojiang Cui
author_facet	Srivarshini Cherukupalli Mohan Tian-Yu Lee Armando E. Giuliano Xiaojiang Cui
author_sort	Srivarshini Cherukupalli Mohan
title	Current Status of Breast Organoid Models
title_short	Current Status of Breast Organoid Models
title_full	Current Status of Breast Organoid Models
title_fullStr	Current Status of Breast Organoid Models
title_full_unstemmed	Current Status of Breast Organoid Models
title_sort	current status of breast organoid models
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5f47b17769754222a1e82e54d208dedb
work_keys_str_mv	AT srivarshinicherukupallimohan currentstatusofbreastorganoidmodels AT tianyulee currentstatusofbreastorganoidmodels AT armandoegiuliano currentstatusofbreastorganoidmodels AT xiaojiangcui currentstatusofbreastorganoidmodels
_version_	1718444470694838272

Current Status of Breast Organoid Models

Ejemplares similares