Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production

Abstract Seed germination is a critical and complex process in the plant life cycle. Although previous studies have found that melatonin can promote seed germination under salt stress, the involvement of melatonin in the regulation of proteomic changes remains poorly understood. In this study, a tot...

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Autores principales:	Na Zhang, Hai-Jun Zhang, Qian-Qian Sun, Yun-Yun Cao, Xingsheng Li, Bing Zhao, Ping Wu, Yang-Dong Guo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f48faab794c44b4b41e308ca160c55c
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spelling	oai:doaj.org-article:5f48faab794c44b4b41e308ca160c55c2021-12-02T16:06:20ZProteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production10.1038/s41598-017-00566-12045-2322https://doaj.org/article/5f48faab794c44b4b41e308ca160c55c2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00566-1https://doaj.org/toc/2045-2322Abstract Seed germination is a critical and complex process in the plant life cycle. Although previous studies have found that melatonin can promote seed germination under salt stress, the involvement of melatonin in the regulation of proteomic changes remains poorly understood. In this study, a total of 157 proteins were significantly influenced (ratio ≥ 2 or ≤ −2) by melatonin during seed germination under salt stress using a label-free quantitative technique. Our GO analysis revealed that several pathways were obviously regulated by melatonin, including ribosome biosynthesis, lipid metabolism, carbohydrate metabolism, and storage protein degradation. Not only stress-tolerant proteins but also proteins that produce ATP as part of glycolysis, the citric acid cycle, and the glyoxylate cycle were upregulated by melatonin. Overall, this study provides new evidence that melatonin alleviates the inhibitory effects of NaCl stress on seed germination by promoting energy production. This study is the first to provide insights at the proteomic level into the molecular mechanism of melatonin in response to salt stress in cucumber seeds. This may be helpful to further understand the role of melatonin in cucumber seed germination under stress conditions.Na ZhangHai-Jun ZhangQian-Qian SunYun-Yun CaoXingsheng LiBing ZhaoPing WuYang-Dong GuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Na Zhang Hai-Jun Zhang Qian-Qian Sun Yun-Yun Cao Xingsheng Li Bing Zhao Ping Wu Yang-Dong Guo Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
description	Abstract Seed germination is a critical and complex process in the plant life cycle. Although previous studies have found that melatonin can promote seed germination under salt stress, the involvement of melatonin in the regulation of proteomic changes remains poorly understood. In this study, a total of 157 proteins were significantly influenced (ratio ≥ 2 or ≤ −2) by melatonin during seed germination under salt stress using a label-free quantitative technique. Our GO analysis revealed that several pathways were obviously regulated by melatonin, including ribosome biosynthesis, lipid metabolism, carbohydrate metabolism, and storage protein degradation. Not only stress-tolerant proteins but also proteins that produce ATP as part of glycolysis, the citric acid cycle, and the glyoxylate cycle were upregulated by melatonin. Overall, this study provides new evidence that melatonin alleviates the inhibitory effects of NaCl stress on seed germination by promoting energy production. This study is the first to provide insights at the proteomic level into the molecular mechanism of melatonin in response to salt stress in cucumber seeds. This may be helpful to further understand the role of melatonin in cucumber seed germination under stress conditions.
format	article
author	Na Zhang Hai-Jun Zhang Qian-Qian Sun Yun-Yun Cao Xingsheng Li Bing Zhao Ping Wu Yang-Dong Guo
author_facet	Na Zhang Hai-Jun Zhang Qian-Qian Sun Yun-Yun Cao Xingsheng Li Bing Zhao Ping Wu Yang-Dong Guo
author_sort	Na Zhang
title	Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
title_short	Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
title_full	Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
title_fullStr	Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
title_full_unstemmed	Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
title_sort	proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5f48faab794c44b4b41e308ca160c55c
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_version_	1718385026463170560

Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production

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