Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts o...

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Autores principales:	Lukač Bojan, Knežević Aleksandra, Milić Nenad, Krnjaić Dejan, Veljović Ljubiša, Milićević Vesna, Zorić Andrea, Đurić Spomenka, Stanojević Maja, Nišavić Jakov
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	porcine circovirus 2 porcine parvovirus nucleotide sequence republic of srpska bosnia and herzegovina Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/5f5242f9be0a485da18fb5f46aac67a6
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spelling	oai:doaj.org-article:5f5242f9be0a485da18fb5f46aac67a62021-11-17T21:27:50ZMolecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina1820-744810.1515/acve-2016-0004https://doaj.org/article/5f5242f9be0a485da18fb5f46aac67a62016-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0004https://doaj.org/toc/1820-7448The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.Lukač BojanKnežević AleksandraMilić NenadKrnjaić DejanVeljović LjubišaMilićević VesnaZorić AndreaĐurić SpomenkaStanojević MajaNišavić JakovSciendoarticleporcine circovirus 2porcine parvovirusnucleotide sequencerepublic of srpskabosnia and herzegovinaVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 1, Pp 51-60 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	porcine circovirus 2 porcine parvovirus nucleotide sequence republic of srpska bosnia and herzegovina Veterinary medicine SF600-1100
spellingShingle	porcine circovirus 2 porcine parvovirus nucleotide sequence republic of srpska bosnia and herzegovina Veterinary medicine SF600-1100 Lukač Bojan Knežević Aleksandra Milić Nenad Krnjaić Dejan Veljović Ljubiša Milićević Vesna Zorić Andrea Đurić Spomenka Stanojević Maja Nišavić Jakov Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
description	The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.
format	article
author	Lukač Bojan Knežević Aleksandra Milić Nenad Krnjaić Dejan Veljović Ljubiša Milićević Vesna Zorić Andrea Đurić Spomenka Stanojević Maja Nišavić Jakov
author_facet	Lukač Bojan Knežević Aleksandra Milić Nenad Krnjaić Dejan Veljović Ljubiša Milićević Vesna Zorić Andrea Đurić Spomenka Stanojević Maja Nišavić Jakov
author_sort	Lukač Bojan
title	Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
title_short	Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
title_full	Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
title_fullStr	Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
title_full_unstemmed	Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina
title_sort	molecular detection of pcv2 and ppv in pigs in republic of srpska, bosnia and herzegovina
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/5f5242f9be0a485da18fb5f46aac67a6
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Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

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