A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries

A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries

Ying-Chao Chou,1,2 Yi-Shiun Cheng,1 Yung-Heng Hsu,1,2 Yi-Hsun Yu,1,2 Shih-Jung Liu1,2 1Biomaterials Lab, Department of Mechanical Engineering, Chang Gung University, 2Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan Abstract: Biodegradable poly([D,L]-lactide-co-glyco...

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Autores principales: Chou YC, Cheng YS, Hsu YH, Yu YH, Liu SJ
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
artificial periosteum
biodegradable poly([d
l]-lactide-co-glycolide) drug-eluting nanofibers
polycaprolactone stents
segmental long bone open fractures
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/5f530840492f46df97ef73858c2d0918
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spelling oai:doaj.org-article:5f530840492f46df97ef73858c2d09182021-12-02T01:13:34ZA bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries1178-2013https://doaj.org/article/5f530840492f46df97ef73858c2d09182016-03-01T00:00:00Zhttps://www.dovepress.com/a-bio-artificial-polydl-lactide-co-glycolide-drug-eluting-nanofibrous--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ying-Chao Chou,1,2 Yi-Shiun Cheng,1 Yung-Heng Hsu,1,2 Yi-Hsun Yu,1,2 Shih-Jung Liu1,2 1Biomaterials Lab, Department of Mechanical Engineering, Chang Gung University, 2Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan Abstract: Biodegradable poly([D,L]-lactide-co-glycolide) (PLGA) nanofibrous membrane embedded with two drug-to-polymer weight ratios, namely 1:3 and 1:6, which comprised PLGA 180 mg, lidocaine 20 mg, vancomycin 20 mg, and ceftazidime 20 mg, and PLGA 360 mg, lidocaine 20 mg, vancomycin 20 mg, and ceftazidime 20 mg, respectively, was produced as an artificial periosteum in the treatment of segmental femoral fractures. The nanofibrous membrane’s drug release behavior was assessed in vitro using high-performance liquid chromatography and the disk-diffusion method. A femoral segmental fracture model with intramedullary Kirschner-wire fixation was established for the in vivo rabbit activity study. Twenty-four rabbits were divided into two groups. Twelve rabbits in group A underwent femoral fracture fixation only, and 12 rabbits in group B underwent femoral fracture fixation and were administered the drug-loaded nanofibers. Radiographs obtained at 2, 6, and 12 weeks postoperatively were used to assess the bone unions. The total activity counts in animal behavior cages were also examined to evaluate the clinical performance of the rabbits. After the animals were euthanized, both femoral shafts were harvested and assessed for their torque strengths and toughness. The daily in vitro release curve for lidocaine showed that the nanofibers eluted effective levels of lidocaine for longer than 3 weeks. The bioactivity studies of vancomycin and ceftazidime showed that both antibiotics had effective and sustained bactericidal capacities for over 30 days. The findings from the in vivo animal activity study suggested that the rabbits with the artificial drug-eluting periosteum exhibited statistically increased levels of activity and better clinical performance outcomes compared with the rabbits without the artificial periosteum. In conclusion, this artificial drug-eluting periosteum may eventually be used for the treatment of open fractures. Keywords: artificial periosteum, biodegradable poly([D,L]-lactide-co-glycolide) drug-eluting nanofibers, polycaprolactone stents, segmental long bone open fracturesChou YCCheng YSHsu YHYu YHLiu SJDove Medical Pressarticleartificial periosteumbiodegradable poly([dl]-lactide-co-glycolide) drug-eluting nanofiberspolycaprolactone stentssegmental long bone open fracturesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 941-953 (2016)
institution DOAJ
collection DOAJ
language EN
topic artificial periosteum
biodegradable poly([d
l]-lactide-co-glycolide) drug-eluting nanofibers
polycaprolactone stents
segmental long bone open fractures
Medicine (General)
R5-920
spellingShingle artificial periosteum
biodegradable poly([d
l]-lactide-co-glycolide) drug-eluting nanofibers
polycaprolactone stents
segmental long bone open fractures
Medicine (General)
R5-920
Chou YC
Cheng YS
Hsu YH
Yu YH
Liu SJ
A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
description Ying-Chao Chou,1,2 Yi-Shiun Cheng,1 Yung-Heng Hsu,1,2 Yi-Hsun Yu,1,2 Shih-Jung Liu1,2 1Biomaterials Lab, Department of Mechanical Engineering, Chang Gung University, 2Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan Abstract: Biodegradable poly([D,L]-lactide-co-glycolide) (PLGA) nanofibrous membrane embedded with two drug-to-polymer weight ratios, namely 1:3 and 1:6, which comprised PLGA 180 mg, lidocaine 20 mg, vancomycin 20 mg, and ceftazidime 20 mg, and PLGA 360 mg, lidocaine 20 mg, vancomycin 20 mg, and ceftazidime 20 mg, respectively, was produced as an artificial periosteum in the treatment of segmental femoral fractures. The nanofibrous membrane’s drug release behavior was assessed in vitro using high-performance liquid chromatography and the disk-diffusion method. A femoral segmental fracture model with intramedullary Kirschner-wire fixation was established for the in vivo rabbit activity study. Twenty-four rabbits were divided into two groups. Twelve rabbits in group A underwent femoral fracture fixation only, and 12 rabbits in group B underwent femoral fracture fixation and were administered the drug-loaded nanofibers. Radiographs obtained at 2, 6, and 12 weeks postoperatively were used to assess the bone unions. The total activity counts in animal behavior cages were also examined to evaluate the clinical performance of the rabbits. After the animals were euthanized, both femoral shafts were harvested and assessed for their torque strengths and toughness. The daily in vitro release curve for lidocaine showed that the nanofibers eluted effective levels of lidocaine for longer than 3 weeks. The bioactivity studies of vancomycin and ceftazidime showed that both antibiotics had effective and sustained bactericidal capacities for over 30 days. The findings from the in vivo animal activity study suggested that the rabbits with the artificial drug-eluting periosteum exhibited statistically increased levels of activity and better clinical performance outcomes compared with the rabbits without the artificial periosteum. In conclusion, this artificial drug-eluting periosteum may eventually be used for the treatment of open fractures. Keywords: artificial periosteum, biodegradable poly([D,L]-lactide-co-glycolide) drug-eluting nanofibers, polycaprolactone stents, segmental long bone open fractures
format article
author Chou YC
Cheng YS
Hsu YH
Yu YH
Liu SJ
author_facet Chou YC
Cheng YS
Hsu YH
Yu YH
Liu SJ
author_sort Chou YC
title A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
title_short A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
title_full A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
title_fullStr A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
title_full_unstemmed A bio-artificial poly([D,L]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
title_sort bio-artificial poly([d,l]-lactide-co-glycolide) drug-eluting nanofibrous periosteum for segmental long bone open fractures with significant periosteal stripping injuries
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/5f530840492f46df97ef73858c2d0918
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