Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species

Abstract A broad-spectrum reactive oxygen species (ROS)-scavenging hybrid material (CASCADE) was developed by sequential adsorption of heparin (HEP) and poly(L-lysine) (PLL) polyelectrolytes together with superoxide dismutase (SOD) and horseradish peroxidase (HRP) antioxidant enzymes on layered doub...

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Autores principales:	Marko Pavlovic, Szabolcs Muráth, Xénia Katona, Nizar B. Alsharif, Paul Rouster, József Maléth, Istvan Szilagyi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f56db5096d54a3a834ffc02c668dbb6
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spelling	oai:doaj.org-article:5f56db5096d54a3a834ffc02c668dbb62021-12-02T14:28:14ZNanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species10.1038/s41598-021-83819-42045-2322https://doaj.org/article/5f56db5096d54a3a834ffc02c668dbb62021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83819-4https://doaj.org/toc/2045-2322Abstract A broad-spectrum reactive oxygen species (ROS)-scavenging hybrid material (CASCADE) was developed by sequential adsorption of heparin (HEP) and poly(L-lysine) (PLL) polyelectrolytes together with superoxide dismutase (SOD) and horseradish peroxidase (HRP) antioxidant enzymes on layered double hydroxide (LDH) nanoclay support. The synthetic conditions were optimized so that CASCADE possessed remarkable structural (no enzyme leakage) and colloidal (excellent resistance against salt-induced aggregation) stability. The obtained composite was active in decomposition of both superoxide radical anions and hydrogen peroxide in biochemical assays revealing that the strong electrostatic interaction with the functionalized support led to high enzyme loadings, nevertheless, it did not interfere with the native enzyme conformation. In vitro tests demonstrated that ROS generated in human cervical adenocarcinoma cells were successfully consumed by the hybrid material. The cellular uptake was not accompanied with any toxicity effects, which makes the developed CASCADE a promising candidate for treatment of oxidative stress-related diseases.Marko PavlovicSzabolcs MuráthXénia KatonaNizar B. AlsharifPaul RousterJózsef MaléthIstvan SzilagyiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marko Pavlovic Szabolcs Muráth Xénia Katona Nizar B. Alsharif Paul Rouster József Maléth Istvan Szilagyi Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
description	Abstract A broad-spectrum reactive oxygen species (ROS)-scavenging hybrid material (CASCADE) was developed by sequential adsorption of heparin (HEP) and poly(L-lysine) (PLL) polyelectrolytes together with superoxide dismutase (SOD) and horseradish peroxidase (HRP) antioxidant enzymes on layered double hydroxide (LDH) nanoclay support. The synthetic conditions were optimized so that CASCADE possessed remarkable structural (no enzyme leakage) and colloidal (excellent resistance against salt-induced aggregation) stability. The obtained composite was active in decomposition of both superoxide radical anions and hydrogen peroxide in biochemical assays revealing that the strong electrostatic interaction with the functionalized support led to high enzyme loadings, nevertheless, it did not interfere with the native enzyme conformation. In vitro tests demonstrated that ROS generated in human cervical adenocarcinoma cells were successfully consumed by the hybrid material. The cellular uptake was not accompanied with any toxicity effects, which makes the developed CASCADE a promising candidate for treatment of oxidative stress-related diseases.
format	article
author	Marko Pavlovic Szabolcs Muráth Xénia Katona Nizar B. Alsharif Paul Rouster József Maléth Istvan Szilagyi
author_facet	Marko Pavlovic Szabolcs Muráth Xénia Katona Nizar B. Alsharif Paul Rouster József Maléth Istvan Szilagyi
author_sort	Marko Pavlovic
title	Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
title_short	Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
title_full	Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
title_fullStr	Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
title_full_unstemmed	Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
title_sort	nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5f56db5096d54a3a834ffc02c668dbb6
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Nanocomposite-based dual enzyme system for broad-spectrum scavenging of reactive oxygen species

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