Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata

Ocean warming and ocean acidification (OA) are direct consequences of climate change and affect coral reefs worldwide. While the effect of ocean warming manifests itself in increased frequency and severity of coral bleaching, the effects of ocean acidification on corals are less clear. In particular...

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Autores principales:	Marcelle Muniz Barreto, Maren Ziegler, Alexander Venn, Eric Tambutté, Didier Zoccola, Sylvie Tambutté, Denis Allemand, Chakkiath Paul Antony, Christian R. Voolstra, Manuel Aranda
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	climate change coral microbiome 16S rDNA 16S rRNA coral holobiont Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5f5daea03e054ba9874bd4fb61ce97cd
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spelling	oai:doaj.org-article:5f5daea03e054ba9874bd4fb61ce97cd2021-12-01T01:25:41ZEffects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata1664-302X10.3389/fmicb.2021.707674https://doaj.org/article/5f5daea03e054ba9874bd4fb61ce97cd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.707674/fullhttps://doaj.org/toc/1664-302XOcean warming and ocean acidification (OA) are direct consequences of climate change and affect coral reefs worldwide. While the effect of ocean warming manifests itself in increased frequency and severity of coral bleaching, the effects of ocean acidification on corals are less clear. In particular, long-term effects of OA on the bacterial communities associated with corals are largely unknown. In this study, we investigated the effects of ocean acidification on the resident and active microbiome of long-term aquaria-maintained Stylophora pistillata colonies by assessing 16S rRNA gene diversity on the DNA (resident community) and RNA level (active community). Coral colony fragments of S. pistillata were kept in aquaria for 2 years at four different pCO2 levels ranging from current pH conditions to increased acidification scenarios (i.e., pH 7.2, 7.4, 7.8, and 8). We identified 154 bacterial families encompassing 2,047 taxa (OTUs) in the resident and 89 bacterial families including 1,659 OTUs in the active communities. Resident communities were dominated by members of Alteromonadaceae, Flavobacteriaceae, and Colwelliaceae, while active communities were dominated by families Cyclobacteriacea and Amoebophilaceae. Besides the overall differences between resident and active community composition, significant differences were seen between the control (pH 8) and the two lower pH treatments (7.2 and 7.4) in the active community, but only between pH 8 and 7.2 in the resident community. Our analyses revealed profound differences between the resident and active microbial communities, and we found that OA exerted stronger effects on the active community. Further, our results suggest that rDNA- and rRNA-based sequencing should be considered complementary tools to investigate the effects of environmental change on microbial assemblage structure and activity.Marcelle Muniz BarretoMaren ZieglerAlexander VennEric TambuttéDidier ZoccolaSylvie TambuttéDenis AllemandChakkiath Paul AntonyChristian R. VoolstraChristian R. VoolstraManuel ArandaFrontiers Media S.A.articleclimate changecoral microbiome16S rDNA16S rRNAcoral holobiontMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	climate change coral microbiome 16S rDNA 16S rRNA coral holobiont Microbiology QR1-502
spellingShingle	climate change coral microbiome 16S rDNA 16S rRNA coral holobiont Microbiology QR1-502 Marcelle Muniz Barreto Maren Ziegler Alexander Venn Eric Tambutté Didier Zoccola Sylvie Tambutté Denis Allemand Chakkiath Paul Antony Christian R. Voolstra Christian R. Voolstra Manuel Aranda Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata
description	Ocean warming and ocean acidification (OA) are direct consequences of climate change and affect coral reefs worldwide. While the effect of ocean warming manifests itself in increased frequency and severity of coral bleaching, the effects of ocean acidification on corals are less clear. In particular, long-term effects of OA on the bacterial communities associated with corals are largely unknown. In this study, we investigated the effects of ocean acidification on the resident and active microbiome of long-term aquaria-maintained Stylophora pistillata colonies by assessing 16S rRNA gene diversity on the DNA (resident community) and RNA level (active community). Coral colony fragments of S. pistillata were kept in aquaria for 2 years at four different pCO2 levels ranging from current pH conditions to increased acidification scenarios (i.e., pH 7.2, 7.4, 7.8, and 8). We identified 154 bacterial families encompassing 2,047 taxa (OTUs) in the resident and 89 bacterial families including 1,659 OTUs in the active communities. Resident communities were dominated by members of Alteromonadaceae, Flavobacteriaceae, and Colwelliaceae, while active communities were dominated by families Cyclobacteriacea and Amoebophilaceae. Besides the overall differences between resident and active community composition, significant differences were seen between the control (pH 8) and the two lower pH treatments (7.2 and 7.4) in the active community, but only between pH 8 and 7.2 in the resident community. Our analyses revealed profound differences between the resident and active microbial communities, and we found that OA exerted stronger effects on the active community. Further, our results suggest that rDNA- and rRNA-based sequencing should be considered complementary tools to investigate the effects of environmental change on microbial assemblage structure and activity.
format	article
author	Marcelle Muniz Barreto Maren Ziegler Alexander Venn Eric Tambutté Didier Zoccola Sylvie Tambutté Denis Allemand Chakkiath Paul Antony Christian R. Voolstra Christian R. Voolstra Manuel Aranda
author_facet	Marcelle Muniz Barreto Maren Ziegler Alexander Venn Eric Tambutté Didier Zoccola Sylvie Tambutté Denis Allemand Chakkiath Paul Antony Christian R. Voolstra Christian R. Voolstra Manuel Aranda
author_sort	Marcelle Muniz Barreto
title	Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata
title_short	Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata
title_full	Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata
title_fullStr	Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata
title_full_unstemmed	Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata
title_sort	effects of ocean acidification on resident and active microbial communities of stylophora pistillata
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5f5daea03e054ba9874bd4fb61ce97cd
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Effects of Ocean Acidification on Resident and Active Microbial Communities of Stylophora pistillata

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