An assembly funnel makes biomolecular complex assembly efficient.

Like protein folding and crystallization, the self-assembly of complexes is a fundamental form of biomolecular organization. While the number of methods for creating synthetic complexes is growing rapidly, most require empirical tuning of assembly conditions and/or produce low yields. We use coarse-...

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Autores principales:	John Zenk, Rebecca Schulman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f6f9192fae246a79c1006e22ca766ae
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spelling	oai:doaj.org-article:5f6f9192fae246a79c1006e22ca766ae2021-11-25T05:54:59ZAn assembly funnel makes biomolecular complex assembly efficient.1932-620310.1371/journal.pone.0111233https://doaj.org/article/5f6f9192fae246a79c1006e22ca766ae2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0111233https://doaj.org/toc/1932-6203Like protein folding and crystallization, the self-assembly of complexes is a fundamental form of biomolecular organization. While the number of methods for creating synthetic complexes is growing rapidly, most require empirical tuning of assembly conditions and/or produce low yields. We use coarse-grained simulations of the assembly kinetics of complexes to identify generic limitations on yields that arise because of the many simultaneous interactions allowed between the components and intermediates of a complex. Efficient assembly occurs when nucleation is fast and growth pathways are few, i.e. when there is an assembly "funnel". For typical complexes, an assembly funnel occurs in a narrow window of conditions whose location is highly complex specific. However, by redesigning the components this window can be drastically broadened, so that complexes can form quickly across many conditions. The generality of this approach suggests assembly funnel design as a foundational strategy for robust biomolecular complex synthesis.John ZenkRebecca SchulmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e111233 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John Zenk Rebecca Schulman An assembly funnel makes biomolecular complex assembly efficient.
description	Like protein folding and crystallization, the self-assembly of complexes is a fundamental form of biomolecular organization. While the number of methods for creating synthetic complexes is growing rapidly, most require empirical tuning of assembly conditions and/or produce low yields. We use coarse-grained simulations of the assembly kinetics of complexes to identify generic limitations on yields that arise because of the many simultaneous interactions allowed between the components and intermediates of a complex. Efficient assembly occurs when nucleation is fast and growth pathways are few, i.e. when there is an assembly "funnel". For typical complexes, an assembly funnel occurs in a narrow window of conditions whose location is highly complex specific. However, by redesigning the components this window can be drastically broadened, so that complexes can form quickly across many conditions. The generality of this approach suggests assembly funnel design as a foundational strategy for robust biomolecular complex synthesis.
format	article
author	John Zenk Rebecca Schulman
author_facet	John Zenk Rebecca Schulman
author_sort	John Zenk
title	An assembly funnel makes biomolecular complex assembly efficient.
title_short	An assembly funnel makes biomolecular complex assembly efficient.
title_full	An assembly funnel makes biomolecular complex assembly efficient.
title_fullStr	An assembly funnel makes biomolecular complex assembly efficient.
title_full_unstemmed	An assembly funnel makes biomolecular complex assembly efficient.
title_sort	assembly funnel makes biomolecular complex assembly efficient.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5f6f9192fae246a79c1006e22ca766ae
work_keys_str_mv	AT johnzenk anassemblyfunnelmakesbiomolecularcomplexassemblyefficient AT rebeccaschulman anassemblyfunnelmakesbiomolecularcomplexassemblyefficient AT johnzenk assemblyfunnelmakesbiomolecularcomplexassemblyefficient AT rebeccaschulman assemblyfunnelmakesbiomolecularcomplexassemblyefficient
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An assembly funnel makes biomolecular complex assembly efficient.

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