Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?
The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning proce...
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2014
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oai:doaj.org-article:5f7532b108db4020b02a923b8eabad532021-11-25T06:03:02ZIs the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?1932-620310.1371/journal.pone.0105295https://doaj.org/article/5f7532b108db4020b02a923b8eabad532014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25162451/?tool=EBIhttps://doaj.org/toc/1932-6203The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.Marta PokrywczynskaArkadiusz JundzillJan AdamowiczTomasz KowalczykKarolina WardaMarta RasmusLukasz BuchholzSandra KrzyzanowskaPawel NakielskiPawel NakielskiTomasz ChmielewskiMagdalena BodnarAndrzej MarszalekRobert DebskiMalgorzata Frontczak-BaniewiczGrzegorz MikułowskiMaciej NowackiTomasz A KowalewskiTomasz DrewaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105295 (2014) |
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Medicine R Science Q Marta Pokrywczynska Arkadiusz Jundzill Jan Adamowicz Tomasz Kowalczyk Karolina Warda Marta Rasmus Lukasz Buchholz Sandra Krzyzanowska Pawel Nakielski Pawel Nakielski Tomasz Chmielewski Magdalena Bodnar Andrzej Marszalek Robert Debski Malgorzata Frontczak-Baniewicz Grzegorz Mikułowski Maciej Nowacki Tomasz A Kowalewski Tomasz Drewa Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| description |
The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold. |
| format |
article |
| author |
Marta Pokrywczynska Arkadiusz Jundzill Jan Adamowicz Tomasz Kowalczyk Karolina Warda Marta Rasmus Lukasz Buchholz Sandra Krzyzanowska Pawel Nakielski Pawel Nakielski Tomasz Chmielewski Magdalena Bodnar Andrzej Marszalek Robert Debski Malgorzata Frontczak-Baniewicz Grzegorz Mikułowski Maciej Nowacki Tomasz A Kowalewski Tomasz Drewa |
| author_facet |
Marta Pokrywczynska Arkadiusz Jundzill Jan Adamowicz Tomasz Kowalczyk Karolina Warda Marta Rasmus Lukasz Buchholz Sandra Krzyzanowska Pawel Nakielski Pawel Nakielski Tomasz Chmielewski Magdalena Bodnar Andrzej Marszalek Robert Debski Malgorzata Frontczak-Baniewicz Grzegorz Mikułowski Maciej Nowacki Tomasz A Kowalewski Tomasz Drewa |
| author_sort |
Marta Pokrywczynska |
| title |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| title_short |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| title_full |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| title_fullStr |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| title_full_unstemmed |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| title_sort |
is the poly (l- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/5f7532b108db4020b02a923b8eabad53 |
| work_keys_str_mv |
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