Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa

Background and Objective: Garlic is one of the oldest plants that has been used for prevention and treatment of different diseases. Also, pseudomonas aeruginosa is the most important form of human pathogens that is resistant to many different antibiotics and makes some problems in treatment. This st...

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Autores principales:	Z Molana, Z Shahandeh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2003
Materias:	garlic garlic extract pseudomonas aeruginosa antibiogram Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5f786dab6fb94506b9b7b36942042eb5
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spelling	oai:doaj.org-article:5f786dab6fb94506b9b7b36942042eb52021-11-10T09:18:15ZEffect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa1561-41072251-7170https://doaj.org/article/5f786dab6fb94506b9b7b36942042eb52003-07-01T00:00:00Zhttp://jbums.org/article-1-2764-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Background and Objective: Garlic is one of the oldest plants that has been used for prevention and treatment of different diseases. Also, pseudomonas aeruginosa is the most important form of human pathogens that is resistant to many different antibiotics and makes some problems in treatment. This study was done to evaluate the effect of garlic and its extract on pseudomonas aeruginosa. Methods: The standard strain of pseudomonas aeruginosa was prepared and after providing cloves of garlic with different weights (0.5, 1, 1.5 and 2 gr) as raw, frozen and cooked and also preparing microbial suspension from samples equal with McFarland solution (0.5), bacteria was cultured on MH agar and cloves of garlic was put on that and then incubated. Garlic extract with different concentrations (30, 40, 50, 60 and 70 mg/ml) were affected by Nathan agar well diffusion method. Disk-diffusion method was done for determining the sensitivity and resistance of bacteria. Findings: Garlic extract with 40 and 35 mg/ml concentration had bactericidal and bacteriostatic effects on the standard strain of pseudomonas aeruginosa. Also, cloves of garlic with different weight cause the growth inhibition of bacteria but cooked cloves had the least effect on bacteria. Conclusion: Garlic extract with 50 mg/ml concentration cause zone of growth inhibition that is equivalent to the disk of gentamicin (10 microgram). Since, cloves of garlic especially frozen one cause to growth inhibition of bacteria, it is recommended to do more researches about the effective concentration of allicin on pathogens and use of soap, cream and ointment for disinfection of wounds.Z MolanaZ ShahandehBabol University of Medical Sciencesarticlegarlicgarlic extractpseudomonas aeruginosaantibiogramMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 5, Iss 3, Pp 57-62 (2003)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	garlic garlic extract pseudomonas aeruginosa antibiogram Medicine R Medicine (General) R5-920
spellingShingle	garlic garlic extract pseudomonas aeruginosa antibiogram Medicine R Medicine (General) R5-920 Z Molana Z Shahandeh Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa
description	Background and Objective: Garlic is one of the oldest plants that has been used for prevention and treatment of different diseases. Also, pseudomonas aeruginosa is the most important form of human pathogens that is resistant to many different antibiotics and makes some problems in treatment. This study was done to evaluate the effect of garlic and its extract on pseudomonas aeruginosa. Methods: The standard strain of pseudomonas aeruginosa was prepared and after providing cloves of garlic with different weights (0.5, 1, 1.5 and 2 gr) as raw, frozen and cooked and also preparing microbial suspension from samples equal with McFarland solution (0.5), bacteria was cultured on MH agar and cloves of garlic was put on that and then incubated. Garlic extract with different concentrations (30, 40, 50, 60 and 70 mg/ml) were affected by Nathan agar well diffusion method. Disk-diffusion method was done for determining the sensitivity and resistance of bacteria. Findings: Garlic extract with 40 and 35 mg/ml concentration had bactericidal and bacteriostatic effects on the standard strain of pseudomonas aeruginosa. Also, cloves of garlic with different weight cause the growth inhibition of bacteria but cooked cloves had the least effect on bacteria. Conclusion: Garlic extract with 50 mg/ml concentration cause zone of growth inhibition that is equivalent to the disk of gentamicin (10 microgram). Since, cloves of garlic especially frozen one cause to growth inhibition of bacteria, it is recommended to do more researches about the effective concentration of allicin on pathogens and use of soap, cream and ointment for disinfection of wounds.
format	article
author	Z Molana Z Shahandeh
author_facet	Z Molana Z Shahandeh
author_sort	Z Molana
title	Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa
title_short	Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa
title_full	Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa
title_fullStr	Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa
title_full_unstemmed	Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa
title_sort	effect of garlic (allium sativum) and garlic extract on growth inhibition of pseudomonas aeruginosa
publisher	Babol University of Medical Sciences
publishDate	2003
url	https://doaj.org/article/5f786dab6fb94506b9b7b36942042eb5
work_keys_str_mv	AT zmolana effectofgarlicalliumsativumandgarlicextractongrowthinhibitionofpseudomonasaeruginosa AT zshahandeh effectofgarlicalliumsativumandgarlicextractongrowthinhibitionofpseudomonasaeruginosa
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Effect of Garlic (Allium Sativum) and Garlic extract on growth inhibition of Pseudomonas Aeruginosa

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