Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.

Granulocyte colony-stimulating factor (G-CSF) induces stem cells to mobilize to the injury site, which have beneficial effect on tissue repair. The aim of this study was to investigate the effect of G-CSF on the thin endometrium in rat models. In the present study, rats with thin endometrium were di...

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Autores principales:	Jing Zhao, Tian Tian, Qiong Zhang, Yonggang Wang, Yanping Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f7b59f03e2c4954ad234d234ffed2ee
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spelling	oai:doaj.org-article:5f7b59f03e2c4954ad234d234ffed2ee2021-11-18T08:40:44ZUse of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.1932-620310.1371/journal.pone.0082375https://doaj.org/article/5f7b59f03e2c4954ad234d234ffed2ee2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376532/?tool=EBIhttps://doaj.org/toc/1932-6203Granulocyte colony-stimulating factor (G-CSF) induces stem cells to mobilize to the injury site, which have beneficial effect on tissue repair. The aim of this study was to investigate the effect of G-CSF on the thin endometrium in rat models. In the present study, rats with thin endometrium were divided into 4 groups (experimental group I: administrated with G-CSF (40 µg/kg/d) 4-6 hours post-modeling; control group I: administrated with saline 4-6 hours post-modeling; experimental group II: administrated with G-CSF (40 µg/kg/d) 12 days post-modeling; control group II: administrated with saline 12 days post-modeling. The agentia was given once daily and last for 5 days. Endometrial morphology was analyzed by Hematoxylin-Eosin staining, and the regeneration of endometrial cells was evaluated by immunohistochemistry and western-blot with cytokeratin and vimentin. We found that endometrial thickness and morphology presented a significant difference between experimental groups and control groups. No matter when we start with G-CSF, there was a significantly thicker endometrium and stronger expression of cytokeratin/vimintin in the experimental groups compared with the control groups (P<0.01). There were significant thicker endometrial lining and stronger expression of cytokeratin/vimintin in experimental group I than that of experimental group II (P<0.05), but there was no difference in the endometrial lining and the expression of cytokeratin/vimintin between the two control groups (P>0.05). In conclusion, G-CSF can promote the regeneration of endometrial cells in animal research, especially when the G-CSF was administrated earlier.Jing ZhaoTian TianQiong ZhangYonggang WangYanping LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82375 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Zhao Tian Tian Qiong Zhang Yonggang Wang Yanping Li Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
description	Granulocyte colony-stimulating factor (G-CSF) induces stem cells to mobilize to the injury site, which have beneficial effect on tissue repair. The aim of this study was to investigate the effect of G-CSF on the thin endometrium in rat models. In the present study, rats with thin endometrium were divided into 4 groups (experimental group I: administrated with G-CSF (40 µg/kg/d) 4-6 hours post-modeling; control group I: administrated with saline 4-6 hours post-modeling; experimental group II: administrated with G-CSF (40 µg/kg/d) 12 days post-modeling; control group II: administrated with saline 12 days post-modeling. The agentia was given once daily and last for 5 days. Endometrial morphology was analyzed by Hematoxylin-Eosin staining, and the regeneration of endometrial cells was evaluated by immunohistochemistry and western-blot with cytokeratin and vimentin. We found that endometrial thickness and morphology presented a significant difference between experimental groups and control groups. No matter when we start with G-CSF, there was a significantly thicker endometrium and stronger expression of cytokeratin/vimintin in the experimental groups compared with the control groups (P<0.01). There were significant thicker endometrial lining and stronger expression of cytokeratin/vimintin in experimental group I than that of experimental group II (P<0.05), but there was no difference in the endometrial lining and the expression of cytokeratin/vimintin between the two control groups (P>0.05). In conclusion, G-CSF can promote the regeneration of endometrial cells in animal research, especially when the G-CSF was administrated earlier.
format	article
author	Jing Zhao Tian Tian Qiong Zhang Yonggang Wang Yanping Li
author_facet	Jing Zhao Tian Tian Qiong Zhang Yonggang Wang Yanping Li
author_sort	Jing Zhao
title	Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
title_short	Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
title_full	Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
title_fullStr	Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
title_full_unstemmed	Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
title_sort	use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5f7b59f03e2c4954ad234d234ffed2ee
work_keys_str_mv	AT jingzhao useofgranulocytecolonystimulatingfactorforthetreatmentofthinendometriuminexperimentalrats AT tiantian useofgranulocytecolonystimulatingfactorforthetreatmentofthinendometriuminexperimentalrats AT qiongzhang useofgranulocytecolonystimulatingfactorforthetreatmentofthinendometriuminexperimentalrats AT yonggangwang useofgranulocytecolonystimulatingfactorforthetreatmentofthinendometriuminexperimentalrats AT yanpingli useofgranulocytecolonystimulatingfactorforthetreatmentofthinendometriuminexperimentalrats
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Use of granulocyte colony-stimulating factor for the treatment of thin endometrium in experimental rats.

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