Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.

<h4>Background</h4>To discover novel markers for improving the efficacy of pancreatic cancer (PC) diagnosis, the secretome of two PC cell lines (BxPC-3 and MIA PaCa-2) was profiled. UL16 binding protein 2 (ULBP2), one of the proteins identified in the PC cell secretome, was selected for...

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Autores principales:	Ya-Ting Chang, Chih-Ching Wu, Yi-Ming Shyr, Tse-Ching Chen, Tsann-Long Hwang, Ta-Sen Yeh, Kai-Ping Chang, Hao-Ping Liu, Yu-Ling Liu, Ming-Hung Tsai, Yu-Sun Chang, Jau-Song Yu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5f7f7fc9ee8142caaec05b9700b0dd8b
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spelling	oai:doaj.org-article:5f7f7fc9ee8142caaec05b9700b0dd8b2021-11-18T06:53:37ZSecretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.1932-620310.1371/journal.pone.0020029https://doaj.org/article/5f7f7fc9ee8142caaec05b9700b0dd8b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625447/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>To discover novel markers for improving the efficacy of pancreatic cancer (PC) diagnosis, the secretome of two PC cell lines (BxPC-3 and MIA PaCa-2) was profiled. UL16 binding protein 2 (ULBP2), one of the proteins identified in the PC cell secretome, was selected for evaluation as a biomarker for PC detection because its mRNA level was also found to be significantly elevated in PC tissues.<h4>Methods</h4>ULBP2 expression in PC tissues from 67 patients was studied by immunohistochemistry. ULBP2 serum levels in 154 PC patients and 142 healthy controls were measured by bead-based immunoassay, and the efficacy of serum ULBP2 for PC detection was compared with the widely used serological PC marker carbohydrate antigen 19-9 (CA 19-9).<h4>Results</h4>Immunohistochemical analyses revealed an elevated expression of ULPB2 in PC tissues compared with adjacent non-cancerous tissues. Meanwhile, the serum levels of ULBP2 among all PC patients (n = 154) and in early-stage cancer patients were significantly higher than those in healthy controls (p<0.0001). The combination of ULBP2 and CA 19-9 outperformed each marker alone in distinguishing PC patients from healthy individuals. Importantly, an analysis of the area under receiver operating characteristic curves showed that ULBP2 was superior to CA 19-9 in discriminating patients with early-stage PC from healthy controls.<h4>Conclusions</h4>Collectively, our results indicate that ULBP2 may represent a novel and useful serum biomarker for pancreatic cancer primary screening.Ya-Ting ChangChih-Ching WuYi-Ming ShyrTse-Ching ChenTsann-Long HwangTa-Sen YehKai-Ping ChangHao-Ping LiuYu-Ling LiuMing-Hung TsaiYu-Sun ChangJau-Song YuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20029 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ya-Ting Chang Chih-Ching Wu Yi-Ming Shyr Tse-Ching Chen Tsann-Long Hwang Ta-Sen Yeh Kai-Ping Chang Hao-Ping Liu Yu-Ling Liu Ming-Hung Tsai Yu-Sun Chang Jau-Song Yu Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
description	<h4>Background</h4>To discover novel markers for improving the efficacy of pancreatic cancer (PC) diagnosis, the secretome of two PC cell lines (BxPC-3 and MIA PaCa-2) was profiled. UL16 binding protein 2 (ULBP2), one of the proteins identified in the PC cell secretome, was selected for evaluation as a biomarker for PC detection because its mRNA level was also found to be significantly elevated in PC tissues.<h4>Methods</h4>ULBP2 expression in PC tissues from 67 patients was studied by immunohistochemistry. ULBP2 serum levels in 154 PC patients and 142 healthy controls were measured by bead-based immunoassay, and the efficacy of serum ULBP2 for PC detection was compared with the widely used serological PC marker carbohydrate antigen 19-9 (CA 19-9).<h4>Results</h4>Immunohistochemical analyses revealed an elevated expression of ULPB2 in PC tissues compared with adjacent non-cancerous tissues. Meanwhile, the serum levels of ULBP2 among all PC patients (n = 154) and in early-stage cancer patients were significantly higher than those in healthy controls (p<0.0001). The combination of ULBP2 and CA 19-9 outperformed each marker alone in distinguishing PC patients from healthy individuals. Importantly, an analysis of the area under receiver operating characteristic curves showed that ULBP2 was superior to CA 19-9 in discriminating patients with early-stage PC from healthy controls.<h4>Conclusions</h4>Collectively, our results indicate that ULBP2 may represent a novel and useful serum biomarker for pancreatic cancer primary screening.
format	article
author	Ya-Ting Chang Chih-Ching Wu Yi-Ming Shyr Tse-Ching Chen Tsann-Long Hwang Ta-Sen Yeh Kai-Ping Chang Hao-Ping Liu Yu-Ling Liu Ming-Hung Tsai Yu-Sun Chang Jau-Song Yu
author_facet	Ya-Ting Chang Chih-Ching Wu Yi-Ming Shyr Tse-Ching Chen Tsann-Long Hwang Ta-Sen Yeh Kai-Ping Chang Hao-Ping Liu Yu-Ling Liu Ming-Hung Tsai Yu-Sun Chang Jau-Song Yu
author_sort	Ya-Ting Chang
title	Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
title_short	Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
title_full	Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
title_fullStr	Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
title_full_unstemmed	Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.
title_sort	secretome-based identification of ulbp2 as a novel serum marker for pancreatic cancer detection.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/5f7f7fc9ee8142caaec05b9700b0dd8b
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Secretome-based identification of ULBP2 as a novel serum marker for pancreatic cancer detection.

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